April - June 2007 - Kasetsart University

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258 Detection of xa5 resistant gene for bacterial blight resistance by PCR -based marker Since the anther donor plants (BC 1F 1) were confirmed to be heterozygous for the xa5 resistant gene (data not shown), AC-derived plants from BC 1F 1 were also tested for the xa5 gene using PCR-based marker RG556. The PCR product gave monomorphic amplification products of 1,600 bp (Figure 3A). However, after digesting with Hpy CH4 IV, polymorphism of DNA bands between resistant and susceptible plants was detected. Two bands of 1,000 bp and 300 bp (doublet) were found in IRBB5 (bacterial blight resistant variety) while non-digested DNA band (1,600 bp) was shown in <strong>Kasetsart</strong> J. (Nat. Sci.) 41(2) susceptible variety of KDML105 (Figure 3B). Genotyping by PCR-based method revealed none of these six AC-derived plants contained the xa5 gene for bacterial blight resistance (Figure 3B). This result was confirmed by pathogen inoculation test on 45 days old plants grown from healthy seeds of two homozygous lines (obtained by spontaneous chromosome doubling as described in AFLP analysis section) which showed bacterial blight susceptability (data not shown). Sanchez et al. (2000) reported that the distance between RG556 marker and the xa5 gene was 0.8 cM, then the loss of the xa5 gene in six AC-derived plants in the present study possibly Figure 3 PCR analysis of the bacterial blight susceptible variety (KDML105), resistant variety (IRBB5) and AC-derived plants (the samples number 1, 2, 3, 4, 5, 6): (A) Monomorphic bands amplified with primer RG556, (B) PCR products digested with Hpy CH4 IV. M= 1 kb plus DNA marker.
caused by either the recombination of RG556 marker and the xa5 resistant gene or the segregation of gene during gametogenesis. CONCLUSION Two homozygous lines were rapidly achieved by anther culture of BC 1F 1 hybrid (KDML105//IRBB5/KDML105) and subsequent spontaneous chromosome doubling. In the present study maltose has proven to be a preferred carbon <strong>Kasetsart</strong> J. (Nat. Sci.) 41(2) 259 source compared to sucrose as seen from the significant effect on callus formation. Furthermore, one-step plantlet formation was promoted when callus induction medium supplemented with combination of maltose and NAA was used. Although the percentages of callusing anther and callus proliferation were increased by adding 2,4-D to NAA containing induction medium, the percentages of organs formation as well as complete plantlet formation were very low, moreover one-step plantlet formation did not Figure 4 AFLP fingerprint of the bacterial blight susceptible variety (KDML105), resistant variety (IRBB5) and AC-derived plants (the samples number 1, 2, 3, 4, 5, 6) generated by different primer combinations: (A) E-AGG / M-CTC primer (B) E-AAG / M-CAA primer (C) E- AAG / M-CAG primer, M = 25 bp DNA size marker (Life Technologies); arrows indicate polymorphic bands specific for KDML105.
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April - June 2007 Volume 41 Number
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KASETSART JOURNAL NATURAL SCIENCE T
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Kasetsart J. (Nat. Sci.) 41 : 205 -
Page 7 and 8: harvesting time which started from
Page 9 and 10: y Chen and Paull (2000). Figure 1 a
Page 11 and 12: pineapple fruit allowed the accumul
Page 13 and 14: Kasetsart J. (Nat. Sci.) 41 : 213 -
Page 15 and 16: Kasetsart J. (Nat. Sci.) 41(2) 215
Page 17 and 18: Cluster analysis Cluster analysis u
Page 21 and 22: Table 3 (Continued) 1 2 3 4 5 6 7 8
Page 25 and 26: CONCLUSION AFLP markers classified
Page 29 and 30: difference of soil texture used in
Page 31 and 32: under wet soil condition planting.
Page 33 and 34: tropics. Following the expansion, w
Page 35 and 36: sealed in a plastic box with 1 cm w
Page 37 and 38: moisture content increment after so
Page 39 and 40: Frequency Frequency 30 20 10 0 20.0
Page 41 and 42: School, Kasetsart University. The a
Page 43 and 44: for combining ability of lines (Lam
Page 45 and 46: selective mass sibbing within indiv
Page 47 and 48: Although most of S 2-interfamily hy
Page 49 and 50: composite sets as used in this stud
Page 53 and 54: days. The numbers of calli producin
Page 55 and 56: the report of Ching (1982) showing
Page 57: clearly amplified bands generated b
Page 61 and 62: Kuhlmann, V. and B. Foroughi-Wehr.
Page 63 and 64: Xanthomonas fuscans subsp. aurantif
Page 67 and 68: was collected and washed with 70% e
Page 69 and 70: expected size was amplified with 35
Page 71 and 72: eaction technique has been used for
Page 73 and 74: Schaad, N.W., D. Opgenorth and P. G
Page 75 and 76: MATERIALS AND METHODS Model descrip
Page 79 and 80: calculated TF value calculated TF v
Page 81 and 82: sincere gratitude and deep apprecia
Page 83 and 84: 1989). In monogastrics, the inclusi
Page 85 and 86: of all LLS samples in this study we
Page 87 and 88: Melbourne, Parkville, Victoria. Goe
Page 89 and 90: considered responsible for low prod
Page 93 and 94: mineral supplementation suggested b
Page 95 and 96: Body weight chane (kg/head) 32 30 2
Page 97 and 98: CONCLUSION AND RECOMMENDATION With
Page 99 and 100: SAS. (Statistical Analysis System).
Page 101 and 102: genes covering a variety of desirab
Page 103 and 104: mg/l NAA and 0.5 mg/l zeatin) incub
Page 105 and 106: 5. Purification by various sucrose
Page 107 and 108: as the culture period increased. So
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culture, pp. 1-20. In L.C. Fowke an
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GC. Analytical methods Total carboh
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ash. The crude hot water polysaccha
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spirulan (H-SP), and a desulfated c
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cells often displayed an increased
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LITERATURE CITED Bell, T.A. and D.V
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for alternative sources of supply.
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BR2.1.2 formed the same clade with
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supplement with glutamate (Iida et
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optimal for S. limacinum BR2.1.2 fo
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fluorescent lamp at 1.5 kLux develo
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Wisconsin, USA). Total extracted RN
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RESULTS Cloning and sequencing of m
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Expression of rmIL-2 and purificati
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other strains of mice have differen
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dose interleukin-2 therapy promotes
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The chitosano-oligosaccharides are
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enzyme and cells were maximized by
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of crude chitosanases. Fortunately,
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Relative activity (%) 100 80 60 40
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Uchida, K. Tateishi, O. Shida and K
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MATERIALS AND METHODS 1. Materials
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without coating and coated with pec
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in Table 5-7. It was found that the
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the cultures at 10,200 × g for 15
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incubation and reached the final pH
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y lysine- and tyrosine-decarboxylas
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during the stages of ripening as su
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As the 25 companies were divided in
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All the data comes up with informat
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as piece “A” or straight as pie
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et al. (1998); Zeng (2000); and Tal
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p 1, i * Kasetsart J. (Nat. Sci.) 4
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c ≤ c2 jq2 j n q p k p * 2, j , ,
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algorithm would consider RM 5, prio
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Kasetsart J. (Nat. Sci.) 41(2) 389
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the maximum deviation of about 10%.
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Stock Quantities Using Heuristic Op
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which dynamically and automatically
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2. Framework architecture and compo
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Risk (VaR) calculation which was im
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Throughput (job/sec) 7 6 5 4 3 2 1
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Speed up 40 35 30 25 20 15 10 5 0 F
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and discovery, resource selection a
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April - June 2007 - Kasetsart University

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