April - June 2007 - Kasetsart University

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344 2 fusion proteins. In the receptor binding assay, the rmIL-2 bound to its receptor showing green fluorescence on the cell surface. This experiment confirmed the rmIL-2 biological activity in binding to its receptor and leading to cell proliferation by XTT assay. The future plan of our project will be the use rmIL-2 as an antigen to raise anti-IL 2 polyclonal antibody and develop the ELISA method for the measurement of mouse IL-2 for further investigation. ACKNOWLEDGEMENTS The authors would like to acknowledge the Centre for Agricultural Biotechnology, Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom for the facility and financial support throughout this study. LITERATURE CITED Adachi, M., M. Ishino, T. Torigoe, Y. Minami, T. Matozaki, T. Miyazaki, T. Taniguchi, Y. Hinoda and K. Imai. 1997. Interleukin-2 induces tyrosine phosphorylation of SHP-2 through IL-2 receptor β chain. Oncogene 14: 1629–1633. Blackman, M.A., M.A. Tigges, M.E. Minie and M.E. Koshland. 1986. A model system for peptide hormone action in differentiation: interleukin-2 induces a B lymphoma to transcribe the J. chain. gene. Cell 47: 609-617. Bradford, M. 1976. A rapid and sensitivity method of measuring microgram quantities of proteins utilizing the principle of protein-dye coupling. Anal. Biochem. 72: 248-264. Brennan, P., J.W. Babbage, B.M. Burgering, B. Groner, K. Reif and D.A. Cantrell. 1997. Phosphatidylinositol 3-kinase couples the interleukin-2 receptor to the cell cycle regulator E2F. Immunity 7: 679–689. Carson, W.E., T.A. Fehniger, S. Haldar, K. Eckhert, M.J. Lindemann and C.F. Lai. 1997. A Kasetsart J. (Nat. Sci.) 41(2) potential role for interleukin-15 in the regulation of human natural killer cell survival. J. Clin. Invest. 99: 937-943. Cook, W.S. and R.H. Unger. 2002. Protein tyrosine phosphatase 1B: a potential leptin resistance factor of obesity. Dev. Cell. 2(4): 385-387. Deng, Y.M., E.T. Bernard and D.R. Willium. 2000. Transmission of porcine endogenous retroviruses in severe combined immunodefficient mice xenotransplanted with fetal porcine pancreatic cells. Transplantation 70: 1010-1016. Eckenberg, R., J.L. Moreau, O. Melnyk and J. Theze. 2000. IL-2R beta agonist P1-30 acts in synergy with IL-2, IL-4, IL-9, and IL-15: biological and molecular effects. J. Immunol. 165: 4312-4318. Gaffen, S.L., S.L. Wang and M.E. Koshland. 1996. Expression of the immunoglobulin J chain in a murine B lymphoma is driven by autocrine production of interleukin-2. Cytokine 8: 513- 524. Gesbert, F., M. Delespine-Carmagnat and J. Bertoglio. 1998. Recent advances in the understanding of interleukin-2 signal transduction. J. Clin. Immunol. 18: 307-320. Giri, J.G., M. Ahdieh, J. Eisenmann, K. Shanebeck, K. Grabstein and S. Kumaki. 1994. Utilization of the b and g chains of the IL-2 receptor by the novel cytokine IL-15. EMBO. J. 13: 2822-2830. Giri, J.G., S. Kumaki, M. Ahdieh, D.J. Friend, A. Loomis and K. Shanebeck. 1995. Identification and cloning of a novel IL-15 binding protein that is structurally related to the a chain of the IL-2 receptor. EMBO. J. 14: 3654-3663. Justement, L.B. 2001. The role of the protein tyrosine phosphatase CD45 in regulation of B lymphocyte activation. Int. Rev. Immunol. 20(6): 713-738. Khatri, V.P., T.A. Fehniger, R.A. Baiocchi, F. Yu, M.H. Shah and D.S. Schiller. 1998. Ultra low
dose interleukin-2 therapy promotes a type 1 cytokine profile in vivo in patients with AIDS and AIDS-associated malignancies. J. Clin. Invest. 101: 1373-1378. Koshland, M.E. 1985. The coming of age of the immunoglobulin J chain. Annu. Rev. Immunol. 3: 425-453. Laemmli, U.K. 1970. Cleavage of structure proteins during the assembly of the head bacteriophage T4. Nature 277: 680-685. Lenardo, M., F.K.-M. Chan, F. Hornung, H. McFarland, R. Siegel and J. Wang. 1999. Mature T lymphocyte apoptosisdimmune regulation in a dynamic and unpredictable environment. Annu. Rev. Immunol. 17: 221- 253. Ma, A. 2000. Pleiotropic functions of IL-15 in innate and adaptive immunity. Mod. Aspects Immunobiol. 1: 102-104. Matesanz, F. and A. Alcina. 1996. Glutamine and tetrapeptide repeat variations affect the biological activity of different mouse interleukin-2 alleles. Eur. J. Immunol. 26(8): 1675-1682. Michelle, M.F., R. Forest and L.M. Kathleen. 2003. IL-2 activation of a PI3K-dependent Kasetsart J. (Nat. Sci.) 41(2) 345 STAT3 serine phosphorylation pathway in primary human T cells. Cell. Signal. 15: 625- 636. Morgan, D.A., F.W. Ruscetti and R. Gallo. 1976. Selective in vitro growth of T lymphocytes from normal human bone marrows. Science. 193: 1007-1008. Robb, R.J., A. Munck and K.A. Smith. 1981. T cell growth factor receptors. Quantitation, specificity, and biological relevance. J. Exp. Med. 154: 1455-1474. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: a Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. Scudiero, D.A., R.H. Shoemaker, K.D. Paull, A. Monks, S. Tierney, T.H. Nofziger, M.J. Currens, D. Seniff and M.R. Boyd. 1988. Evaluation of a soluble tetrazolium/ formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Res. 48: 4827-4833. Smith, K.A. 1989. The interleukin-2 receptor. Annu. Rev. Cell. Biol. 5: 397-425. Stoker, A.W. 2005. Protein tyrosine phosphatases and signalling. J. Endocrinol. 185(1): 19-33.
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April - June 2007 Volume 41 Number
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KASETSART JOURNAL NATURAL SCIENCE T
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Kasetsart J. (Nat. Sci.) 41 : 205 -
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harvesting time which started from
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y Chen and Paull (2000). Figure 1 a
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pineapple fruit allowed the accumul
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Kasetsart J. (Nat. Sci.) 41(2) 215
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Cluster analysis Cluster analysis u
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Table 3 (Continued) 1 2 3 4 5 6 7 8
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CONCLUSION AFLP markers classified
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difference of soil texture used in
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under wet soil condition planting.
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tropics. Following the expansion, w
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sealed in a plastic box with 1 cm w
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moisture content increment after so
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Frequency Frequency 30 20 10 0 20.0
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School, Kasetsart University. The a
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for combining ability of lines (Lam
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selective mass sibbing within indiv
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Although most of S 2-interfamily hy
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composite sets as used in this stud
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Page 53 and 54:
days. The numbers of calli producin
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the report of Ching (1982) showing
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clearly amplified bands generated b
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caused by either the recombination
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Kuhlmann, V. and B. Foroughi-Wehr.
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Xanthomonas fuscans subsp. aurantif
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was collected and washed with 70% e
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expected size was amplified with 35
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eaction technique has been used for
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Schaad, N.W., D. Opgenorth and P. G
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MATERIALS AND METHODS Model descrip
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calculated TF value calculated TF v
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sincere gratitude and deep apprecia
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1989). In monogastrics, the inclusi
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of all LLS samples in this study we
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Melbourne, Parkville, Victoria. Goe
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considered responsible for low prod
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Page 93 and 94: mineral supplementation suggested b
Page 95 and 96: Body weight chane (kg/head) 32 30 2
Page 97 and 98: CONCLUSION AND RECOMMENDATION With
Page 99 and 100: SAS. (Statistical Analysis System).
Page 101 and 102: genes covering a variety of desirab
Page 103 and 104: mg/l NAA and 0.5 mg/l zeatin) incub
Page 105 and 106: 5. Purification by various sucrose
Page 107 and 108: as the culture period increased. So
Page 109 and 110: culture, pp. 1-20. In L.C. Fowke an
Page 111 and 112: Kasetsart J. (Nat. Sci.) 41 : 311 -
Page 113 and 114: GC. Analytical methods Total carboh
Page 115 and 116: ash. The crude hot water polysaccha
Page 117 and 118: spirulan (H-SP), and a desulfated c
Page 121 and 122: cells often displayed an increased
Page 123 and 124: LITERATURE CITED Bell, T.A. and D.V
Page 125 and 126: for alternative sources of supply.
Page 127 and 128: BR2.1.2 formed the same clade with
Page 129 and 130: supplement with glutamate (Iida et
Page 131 and 132: optimal for S. limacinum BR2.1.2 fo
Page 133 and 134: fluorescent lamp at 1.5 kLux develo
Page 137 and 138: Wisconsin, USA). Total extracted RN
Page 139 and 140: RESULTS Cloning and sequencing of m
Page 141 and 142: Expression of rmIL-2 and purificati
Page 143: other strains of mice have differen
Page 147 and 148: The chitosano-oligosaccharides are
Page 149 and 150: enzyme and cells were maximized by
Page 151 and 152: of crude chitosanases. Fortunately,
Page 153 and 154: Relative activity (%) 100 80 60 40
Page 155 and 156: Uchida, K. Tateishi, O. Shida and K
Page 157 and 158: MATERIALS AND METHODS 1. Materials
Page 159 and 160: without coating and coated with pec
Page 161 and 162: in Table 5-7. It was found that the
Page 165 and 166: the cultures at 10,200 × g for 15
Page 167 and 168: incubation and reached the final pH
Page 169 and 170: y lysine- and tyrosine-decarboxylas
Page 171 and 172: during the stages of ripening as su
Page 175 and 176: As the 25 companies were divided in
Page 177 and 178: All the data comes up with informat
Page 179 and 180: as piece “A” or straight as pie
Page 181 and 182: et al. (1998); Zeng (2000); and Tal
Page 183 and 184: p 1, i * Kasetsart J. (Nat. Sci.) 4
Page 185 and 186: c ≤ c2 jq2 j n q p k p * 2, j , ,
Page 187 and 188: algorithm would consider RM 5, prio
Page 189 and 190: Kasetsart J. (Nat. Sci.) 41(2) 389
Page 191 and 192: the maximum deviation of about 10%.
Page 193 and 194: Stock Quantities Using Heuristic Op
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which dynamically and automatically
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2. Framework architecture and compo
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Risk (VaR) calculation which was im
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Throughput (job/sec) 7 6 5 4 3 2 1
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Speed up 40 35 30 25 20 15 10 5 0 F
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and discovery, resource selection a
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April - June 2007 - Kasetsart University

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