April - June 2007 - Kasetsart University

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2 fusion proteins. In the receptor binding assay,
the rmIL-2 bound to its receptor showing green
fluorescence on the cell surface. This experiment
confirmed the rmIL-2 biological activity in binding
to its receptor and leading to cell proliferation by
XTT assay. The future plan of our project will be
the use rmIL-2 as an antigen to raise anti-IL 2
polyclonal antibody and develop the ELISA
method for the measurement of mouse IL-2 for
further investigation.
ACKNOWLEDGEMENTS
The authors would like to acknowledge
the Centre for Agricultural Biotechnology,
Kasetsart University. Kamphaeng Saen Campus,
Nakhon Pathom for the facility and financial
support throughout this study.
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