April - June 2007 - Kasetsart University

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260 occur. By two-steps culturing, the highest percentage of green plant regeneration was obtained (1.29%) from calli induced on N 6 medium without 2,4-D and subsequently cultured on regeneration medium containing MS supplemented with 2 mg/l BAP, 0.2 mg/l NAA, 300 mg/l casein hydrolysate, 15% coconut water, and 30g/l sucrose. The contribution of recurrent parental genome in AC-derived plants was revealed by AFLP analysis. Even though these ACderived plants did not contain a bacterial blight resistant gene (xa5) when screened by PCR-based RG556 marker, other desirable traits such as dwarf plant type, photoperiod insensitive response and aroma, characteristic of the parents could be obtained from them. ACKNOWLEDGEMENTS This research was financially supported by Kasetsart University Research and Development Institute (KURDI) and Thesis and Dissertation Support Fund, Graduate School, Kasetsart University. The authors also would like to thank Dr. Kanchana Klakhaeng, Patumtani Rice Research Center for supplying rice seeds, and Dr. Nongrat Nilpanit, Division of Plant Pathology and Microbiology, Department of Agriculture, for providing pathogen inoculation test. LITERATURE CITED Agrawal, R.K., R.N. Pandey and V.P. Agrawal. 1992. Isolation of DNA from Choerospondias asillaris leaves. Biotech. Biodiv. Lett. 2: 19-24. Ball, S.T., H.P. Zhou and C.F. Konzak. 1993. Influence of 2,4-D, IAA and duration of callus induction in anther culture of spring wheat. Plant Sci. 90: 195-200. Bhojwani, S., H. Pande and A. Raina. 2001. Factors affecting androgenesis in indica rice. Available Source : http://www.bibd.un.- Kasetsart J. (Nat. Sci.) 41(2) giessen.de/gdoe. September 21, 2001. Bishnoi, U.S., R.K. Jain, K.R. Gupta, V.K. Chowdhury and J.B. Chowdhury. 2000. High frequency androgenesis in indica x basmati rice hybrids using liquid culture media. Plant Cell Tiss. Org. Cult. 61: 153-159. Boonintara, J. 2004. Anther culture of F 1 hybrid and F 2 progenies of aromatic rice (Oryza sativa L.) for the production of double haploid with photoperiod insensitive character. MS. Thesis, Kasetsart University, Bangkok. Chen, C.C. 1978. Effect of sucrose concentration on plant production in anther culture of rice.Crop Sci.18: 905-906. Chu, C.C. 1978. The N 6 medium and its application to anther culture of cereal crops, pp. 43-50. In Proceeding of Symposium on Plant Tissue Culture. Science Press, Beijing. Datta, S.K., K. Datta and I. Potrykus. 1990. Embryogenesis and plant formation from microspores of both ‘indica’ and ‘japonica’ rice (Oryza sativa). Plant Sci. 67: 83-88. Guiderdoni, E., E. Galinato, J. Luistro and G. Vergara. 1991. Anther culture of tropical japonica x indica hybrids of rice (Oryza sativa L.). Euphytica 62: 219-224. Guzman, M. and F.J. Zapata-Arias. 2000. Increasing anther culture efficiency in rice (Oryza sativa L.) using anthers from ratooned plants. Plant Sci. 151: 107-114. Huang, N., E.R. Angles, J. Domingo, G. Magpantay, S. Singh, G. Zhang, K. Kumaravadiel, J. Bennett and G.S. Khush. 1997. Pyramiding of bacterial blight resistance gene in rice:marker-assisted selection using RFLP and PCR. Theor. Appl. Genet. 95: 313- 320. Khanna, H.K. and K. Raina. 1998. Genotype x culture medium interaction effects on regeneration response of three indica rice cultivars. Plant Cell Tiss. Org. Cult. 52: 145- 153.
Kuhlmann, V. and B. Foroughi-Wehr. 1989. Production of doubled haploid lines in frequencies sufficient for barley breeding programs. Plant Cell Rep. 8: 78-81. Lentini, Z., P. Reyes, C.P. Martinez and W.M. Roca. 1995. Androgenesis in highly recalcitrant rice genotype with maltose and silver nitrate. Plant Sci. 110: 127-138. Lertvichai, R. 1995. Anther culture of hybrid rice (Lemont/Khao Dawk Mali 105). MS. Thesis, Kasetsart University, Bangkok. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant 15: 473- 492. Pakdeechanuan, P. 1997. Anther culture of F(1) hybrid rice (KDML 105/RD23) for studying for brown planthopper (Nilaparvata lugens) resistance. MS. Thesis, Kasetsart University, Bangkok. Roy,B. and A.B. Mandal. 2005. Anther culture response in indica rice and variations in major agronomic charaters among the androclones of a scented cultivar , Karnal local. African J. Biotech. 4(3): 235-240. Kasetsart J. (Nat. Sci.) 41(2) 261 Sanchez, A.C., D.S. Brar, N. Huang, Z. Li and G.S. Khush. 2000. Sequence tagged site markerassisted selection for three bacterial blight resistance gene in rice. Crop Sci. 40: 792-797. Sopory, S.K. and M. Munshi. 1996. Anther culture, pp. 145-176. In S.M Jain, S.K. Sopory and R.E. Veilleux (eds.). In Vitro Haploid Production in Higher Plants, vol.1. Kluwer Academic Publishers, Netherlands. Vos, P., R. Hogers, M. Bleeker, M. Reijans, T. Van de Lee, M. Hornes, A. Frijters, J. Pot, J. Peleman, M. Kuiper and M. Zabeau. 1995. AFLP: a new technique for DNA fingerprinting. Nucl. Acids Res. 23: 4407- 4414. Xie, J., M. Gao, Q. Cai, X. Cheng , Y. Shen and Z. Liang. 1995. Improved isolated microspore culture efficiency in medium with maltose and optimized growth regulator combination in japonica rice (Oryza sativa L.). Plant Cell Tiss. Org. Cult. 42: 245-250. Zhao, J., C. Zhou and H.Y. Yang. 1999. In vitro development of early proembryos and plant regeneration via microculture in Oryza sativa. Plant Cell Tiss. Org. Cult. 55: 167-174.
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April - June 2007 Volume 41 Number
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KASETSART JOURNAL NATURAL SCIENCE T
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Kasetsart J. (Nat. Sci.) 41 : 205 -
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harvesting time which started from
Page 9 and 10: y Chen and Paull (2000). Figure 1 a
Page 11 and 12: pineapple fruit allowed the accumul
Page 13 and 14: Kasetsart J. (Nat. Sci.) 41 : 213 -
Page 15 and 16: Kasetsart J. (Nat. Sci.) 41(2) 215
Page 17 and 18: Cluster analysis Cluster analysis u
Page 21 and 22: Table 3 (Continued) 1 2 3 4 5 6 7 8
Page 25 and 26: CONCLUSION AFLP markers classified
Page 29 and 30: difference of soil texture used in
Page 31 and 32: under wet soil condition planting.
Page 33 and 34: tropics. Following the expansion, w
Page 35 and 36: sealed in a plastic box with 1 cm w
Page 37 and 38: moisture content increment after so
Page 39 and 40: Frequency Frequency 30 20 10 0 20.0
Page 41 and 42: School, Kasetsart University. The a
Page 43 and 44: for combining ability of lines (Lam
Page 45 and 46: selective mass sibbing within indiv
Page 47 and 48: Although most of S 2-interfamily hy
Page 49 and 50: composite sets as used in this stud
Page 53 and 54: days. The numbers of calli producin
Page 55 and 56: the report of Ching (1982) showing
Page 57 and 58: clearly amplified bands generated b
Page 59: caused by either the recombination
Page 63 and 64: Xanthomonas fuscans subsp. aurantif
Page 67 and 68: was collected and washed with 70% e
Page 69 and 70: expected size was amplified with 35
Page 71 and 72: eaction technique has been used for
Page 73 and 74: Schaad, N.W., D. Opgenorth and P. G
Page 75 and 76: MATERIALS AND METHODS Model descrip
Page 79 and 80: calculated TF value calculated TF v
Page 81 and 82: sincere gratitude and deep apprecia
Page 83 and 84: 1989). In monogastrics, the inclusi
Page 85 and 86: of all LLS samples in this study we
Page 87 and 88: Melbourne, Parkville, Victoria. Goe
Page 89 and 90: considered responsible for low prod
Page 93 and 94: mineral supplementation suggested b
Page 95 and 96: Body weight chane (kg/head) 32 30 2
Page 97 and 98: CONCLUSION AND RECOMMENDATION With
Page 99 and 100: SAS. (Statistical Analysis System).
Page 101 and 102: genes covering a variety of desirab
Page 103 and 104: mg/l NAA and 0.5 mg/l zeatin) incub
Page 105 and 106: 5. Purification by various sucrose
Page 107 and 108: as the culture period increased. So
Page 109 and 110: culture, pp. 1-20. In L.C. Fowke an
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GC. Analytical methods Total carboh
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ash. The crude hot water polysaccha
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spirulan (H-SP), and a desulfated c
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cells often displayed an increased
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LITERATURE CITED Bell, T.A. and D.V
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for alternative sources of supply.
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BR2.1.2 formed the same clade with
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supplement with glutamate (Iida et
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optimal for S. limacinum BR2.1.2 fo
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fluorescent lamp at 1.5 kLux develo
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Wisconsin, USA). Total extracted RN
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RESULTS Cloning and sequencing of m
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Expression of rmIL-2 and purificati
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other strains of mice have differen
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dose interleukin-2 therapy promotes
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The chitosano-oligosaccharides are
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enzyme and cells were maximized by
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of crude chitosanases. Fortunately,
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Relative activity (%) 100 80 60 40
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Uchida, K. Tateishi, O. Shida and K
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MATERIALS AND METHODS 1. Materials
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without coating and coated with pec
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in Table 5-7. It was found that the
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the cultures at 10,200 × g for 15
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incubation and reached the final pH
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y lysine- and tyrosine-decarboxylas
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during the stages of ripening as su
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As the 25 companies were divided in
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All the data comes up with informat
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as piece “A” or straight as pie
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et al. (1998); Zeng (2000); and Tal
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p 1, i * Kasetsart J. (Nat. Sci.) 4
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c ≤ c2 jq2 j n q p k p * 2, j , ,
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algorithm would consider RM 5, prio
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Kasetsart J. (Nat. Sci.) 41(2) 389
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the maximum deviation of about 10%.
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Stock Quantities Using Heuristic Op
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which dynamically and automatically
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2. Framework architecture and compo
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Risk (VaR) calculation which was im
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Throughput (job/sec) 7 6 5 4 3 2 1
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Speed up 40 35 30 25 20 15 10 5 0 F
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and discovery, resource selection a
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April - June 2007 - Kasetsart University

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