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April - June 2007 - Kasetsart University

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286<br />

was found on cellulose. These results were similar<br />

to those reported by Jaurena and Pichard (2001).<br />

Beta-carotene and mimosine content in<br />

leucaena silage<br />

β-carotene of LLS increased significantly<br />

(Table 5). Even though no clear explanation can<br />

be given, the result was in agreement with that<br />

reported by Peterson et al. (1935; cited by Watson<br />

and Nash, 1960) who found the increment of<br />

carotene in alfalfa ensiling with acid. However,<br />

Hellbery (1945; cited by Watson and Nash, 1960)<br />

reported that 11-75% of carotene was loss by<br />

oxidation during fermentation. The extent of loss<br />

depended on oxygen content and temperature in<br />

the silo.<br />

Fermentation decreased 91-93% of<br />

mimosine. The length of ensiling had no effect<br />

on mimosine loss. The result was in agreement<br />

with that reported by Hongo et al. (1986) and<br />

Sunagawa et al. (1989) who found mimosine<br />

reduction over 90% in LLS either with or without<br />

additives. The reduction of mimosine by ensiling<br />

being higher than by sun drying (14.5-51.1% of<br />

the original samples) was reported by Panja<br />

(1983), Parkwiwat (1984) and Wee and Wang<br />

(1987). These results indicated that LLS is an<br />

interesting alternative for feed preservation.<br />

CONCLUSION<br />

The ensiling of LL by mixing with 20%<br />

<strong>Kasetsart</strong> J. (Nat. Sci.) 41(2)<br />

Table 5 β-carotene and mimosine content in leucaena mixed with rice bran and ensiled at different<br />

durations.<br />

Ensiling period β-carotene Mimosine<br />

(days) mg/kg DM % increment % DM % lost<br />

0 88.50a 0.00 1.79b 0.00<br />

21 99.92ab 12.90 0.13a 92.74<br />

51 116.29bc 31.40 0.14a 92.18<br />

81 120.28c 35.91 0.16a 91.06<br />

111 105.21abc 18.88 0.12a 93.30<br />

Value in a column with different superscripts differ significantly (p

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