April - June 2007 - Kasetsart University

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Figure 2B demonstrates the effect of
glucose concentration. Cell mass depended on
glucose concentrations and was maximal with 7%
(cell mass 28.3 g/l). However, the highest DHA
production was obtained in 5% glucose (732
mg/l) making up 50.6% of TFA. When glucose
concentration was increased to 7%, the proportion
of DHA (641.1 mg/l) was 44.6% of TFA.
2. Nitrogen source
Further experiments used 5% glucose
and 0.5% yeast extract to determine the effect of
the nitrogen source in Figure 3A. Results revealed
CDW (g/l); DHA (% of TFA))
CDW (g/l); DHA (% of TFA))
60
50
40
30
20
10
0
60
50
40
30
20
10
0
Fructose Glucose Glucose
Syrup
Kasetsart J. (Nat. Sci.) 41(2)
Carbon source
that among complex nitrogen sources (1%
peptone) was the best in supporting growth for
both CDW (20.9 g/l) and DHA (828.2 mg/l).
Soybean meal and skimmed milk although
relatively good nitrogen source for CDW but DHA
production was considerably lower at 441.8 and
545.9 mg /l, respectively. In the medium
containing 0.2% MSG, BR2.1.2 grew at 20.3 g/l
and produced DHA 768.5 mg/l, almost the same
levels as supported by 1% peptone. This result
agrees with those using with Thraustochytriun
aureum ATCC 34304 that grew well in medium
containing glucose, peptone, yeast extract and
Molasses Sucrose Sugar Cane
Juice
CDW (g/l) DHA (% of TFA) DHA Production (mg/l)
2 3 4 5 6 7
Glucose concentration (%)
CDW (g/l) DHA (% of TFA) DHA Production (mg/l)
Figure 2 Effect of (A) carbon sources and (B) glucose concentration on growth and DHA production
by S. limacinum BR2.1.2 cultivated at room temperature in shaker 140 rpm.
(A)
(B)
450
400
350
300
250
200
150
100
50
0
800
700
600
500
400
300
200
100
0
DHA Production (mg/l))
DHA Production (mg/l))