columbia river basin fish and wildlife program annual ...

PROJECTNUMBER TITLE PROJFCT STATUSSCHEDULE AND MILESTONES89-32 5. Determine levels and dosage(cont.) for oral and injectable formsof erythromycin.6. Determine tissue residues.7. Complete registration packagefor FDA.89-40 Analytical Methods for Malachite Date Initiated: December 1988Green - USFWSProiect Officer: J. BauerObiectivel: Accurately determineall three forms of malachitegreen (vernal-leukocarbinolanalogs). Definedetection limits within 10 ppbor less.89-54 Research to Identify EffectiveAnti-Fungal Agents - USFWSProject Officer: R. AustinQbiectives:1. Identify and test alternateanti-fungal agents that cansafely replace malachite greenfor the control and treatmentof fungal infections on eggs,juveniles, and adult springchinook.2. Evaluate test results andrank order tested agents, basedon safety and effectiveness onspring chinook eggs and adults.3. Recommend alternative antifungalagent for U.S. Food andDrug Administration registrationfor use on food fish and eggs.Results/Conclusions: Literature searchon methods for analysis has been done.The selectivity of High PressureLiquid chromatography is being investigated.Date Initiated: September 1989Results/Conclusions:1. List of ten alternative anti-fungalagents selected for testing on salmonideggs. Selection criteria based uponfungicidal activity of agents from invitrotest results.2. Testing initiated on salmonid eggs.1 FY 1990: Test existing analytical methods formeasuring malachite green analogs in water.Determine if developed method will accurately detectmalachite green levels at 10 ppb or less.2. FY 1991: Prepare final report.1. FY 1990: Identify and test anti-fungalagents on eggs, juveniles, and adult springchinook.2. FY 1991: Continue testing anti-fungal agentson adult spring chinook; evaluate safety ofalternative agents tested.3. FY 1991: Evaluate test results and recommendalternative anti-fungal agent having best potentialfor U.S. Food and Drug Administration registration.