Food Lipids: Chemistry, Nutrition, and Biotechnology

Table 3 Solvent Systems that Could Be Used for Separation of Lipids by TLC
Lipid component TLC adsorbent Solvent system Ref.
Complex lipids Silica gel G Chloroform–methanol–water, 25:10:1, v/v/v 120
(animal tissues) Silica gel H Chloroform–methanol–acetic acid–water, 25:15:4:2, v/v/v/v 120
Silica gel H First developing system, pyridine–hexane, 3:1, v/v and second developing
system, chloroform–methanol–pyridine–2 M ammonia, 35:12:65:1, v/v/v/v
120
Complex lipids Silica gel G Acetone–acetic acid–water, 100:2:1, v/v/v 121
(plant tissues) Diisobutyl ketone–acetic acid, 40:25:3.7, v/v/v 4
Simple lipids Silica gel G Hexane–diethyl ether–formic acid, 80:20:2, v/v/v 4, 122
Silica gel G Benzene–diethyl ether–ethyl acetate–acetic acid, 80:10:10:0.2, v/v/v/v 4, 122
Partial acylglycerol Silica gel G
containing 5%
(w/v) boric acid
Chloroform–acetone, 96:4, v/v 4
Neutral plasmalogens Silica gel G Hexane–diethyl ether, 95:5, v/v, in first direction, hexane–diethyl ether, 80:20,
v/v, in second direction
4
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