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Food Lipids: Chemistry, Nutrition, and Biotechnology

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Figure 9 Microbial production of keto acids.<br />

4. Dicarboxylic Acids<br />

Species of yeast, C<strong>and</strong>ida tropicalis, catalyze the conversion of oleic acid to � 9 -<br />

cis-1,18-octadecenedioic acid (85) (Fig. 11) <strong>and</strong> elaidic acid to � 9 -trans-1,18-octadecenedioic<br />

acid (86). An industrial strain of C<strong>and</strong>ida tropicalis has been engineered<br />

to convert methyl myristate, methyl palmitate, methyl stearate, oleic acid <strong>and</strong> erucic<br />

acid to the corresponding �,�-alkanedicarboxylic acids as outlined in Fig. 11 (71).<br />

A mutant of C<strong>and</strong>ida tropicalis efficiently converts linoleic acid into cis-6,cis-<br />

9-octadecadienedioic acid, 3-hydroxy-cis-9,cis-12-octadecadienedioic acid, <strong>and</strong> 3-hydroxy-cis-5,cis-8-octadecadienedioic<br />

acid (87). C<strong>and</strong>ida cloacae cells oxidize long<br />

chain fatty acids to the corresponding dicarboxylic acids (88).<br />

Figure 10 Microbial production of polyunsaturated fatty acids.<br />

Copyright 2002 by Marcel Dekker, Inc. All Rights Reserved.

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