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Food Lipids: Chemistry, Nutrition, and Biotechnology

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ming as compared with chemical neutralization <strong>and</strong> soapstock splitting are due to<br />

lower oil loss, less consumption of water, <strong>and</strong> very little effluent wastes of environmental<br />

concern.<br />

V. PERSPECTIVES<br />

Lipid biotechnology is a very young discipline. Although breathtaking progress has<br />

been made in genetic engineering for alteration of composition of oilseeds <strong>and</strong> oils<br />

(see Chapter 30), the commercial applications of biotechnology for the production<br />

<strong>and</strong> modification of fats <strong>and</strong> oils using whole microbial cells <strong>and</strong> isolated enzymes<br />

are restricted so far to a few areas, such as enzymatic production of cocoa butter<br />

substitutes, human milk fat replacers, <strong>and</strong> bio-ester as well as enzymatic degumming<br />

of fats <strong>and</strong> oils. Factors limiting the application of biotechnology in the area of lipids<br />

include the productivity <strong>and</strong> the cost of downstream processing in microbial processes<br />

<strong>and</strong> the price as well as the reuse properties of enzymes, such as lipases. In<br />

this regard, optimism is raised in the current literature, which contains numerous<br />

research activities aimed at development of novel lipid-based specialty products as<br />

well as mass fat products, such as feedstocks for margarines using biotechnology.<br />

With increasing production <strong>and</strong> use of enzymes in detergents one might foresee the<br />

commercial bulk scale availability of such biocatalysts at competitive prices that<br />

should facilitate their applications in wide areas of lipid biotechnology.<br />

REFERENCES<br />

1. D. J. Murphy. Engineering oil production in rapeseed <strong>and</strong> other oil crops. Trends Biotechnol.<br />

14:206 (1996).<br />

2. C. Ratledge. <strong>Biotechnology</strong> of oils <strong>and</strong> fats, Microbial <strong>Lipids</strong>, Vol. 2 (C. Ratledge <strong>and</strong><br />

S. G. Wilkinson, eds.), Academic Press, London, 1989, p. 567.<br />

3. M. A. Cobelas <strong>and</strong> J. Z. Lechado. <strong>Lipids</strong> in microalage. A review. I. Biochemistry.<br />

Grasas Aceites 40:118 (1989).<br />

4. C. A. Boulton. Extracellular microbial lipids, Microbial <strong>Lipids</strong>, Vol. 2 (C. Ratledge <strong>and</strong><br />

S. G. Wilkinson, eds.), Academic Press, London, 1989, p. 669.<br />

5. C. Ratledge. Microbial oils <strong>and</strong> fats: perspectives <strong>and</strong> prospects. Fats for the Future<br />

(R. C. Cambie, ed.). Ellis Horwood, Chichester, 1989, p. 153.<br />

6. D. Lösel. Fungal lipids. Microbial <strong>Lipids</strong>, Vol. 1 (C. Ratledge <strong>and</strong> S. G. Wilkinson,<br />

eds.). Academic Press, London, 1989, p. 699.<br />

7. M. Kayama, S. Araki, <strong>and</strong> S. Sato. <strong>Lipids</strong> of marine plants. Marine Biogenic <strong>Lipids</strong>,<br />

Fats, <strong>and</strong> Oils, Vol. 2 (R. A. Ackman, ed.). CRC Press, Boca Raton, FL, 1989, p. 3.<br />

8. J. Leman. Oleaginous microorganisms: An assessment of the potential. Adv. Appl. Microbiol.<br />

43:195 (1997).<br />

9. J. Brennan. Mycobacterium <strong>and</strong> other actinomycetes. Microbial <strong>Lipids</strong>, Vol. 1 (C.<br />

Ratledge <strong>and</strong> S. G. Wilkinson, eds.). Academic Press, London, 1989, p. 203.<br />

10. M. Wayman, A. D. Jenkins, <strong>and</strong> A. G. Kormendy. Bacterial production of oils <strong>and</strong> fats.<br />

<strong>Biotechnology</strong> for the Oils <strong>and</strong> Fats Industry (C. Ratledge, P. Dawson <strong>and</strong> J. Rattray,<br />

eds.). American Oil Chemists’ Society, Champaign, IL, 1984, p. 129.<br />

11. B. J. B. Wood. <strong>Lipids</strong> in algae <strong>and</strong> protozoa. Microbial <strong>Lipids</strong>, Vol. 1 (C. Ratledge <strong>and</strong><br />

S. G. Wilkinson, eds.). Academic Press, London, 1989, p. 807.<br />

12. C. H. Gerwick <strong>and</strong> M. W. Bernart. Eicosanoids <strong>and</strong> related compounds from marine<br />

algae. Marine <strong>Biotechnology</strong>, Vol. 1, Pharmaceutical <strong>and</strong> Bioactive Natural Products<br />

(D. H. Attaway <strong>and</strong> O. R. Zaborsky, eds.). Plenum Press, New York, 1993, p. 101.<br />

Copyright 2002 by Marcel Dekker, Inc. All Rights Reserved.

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