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Universidad de Córdoba BIODISPONIBILIDAD MINERAL DE ...

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In vitro estimation of the bioavailability of minerals and trace elements<br />

from foods involves the simulation of gastrointestinal digestion and<br />

measurement of the mineral soluble fraction or the mineral fraction that dialyses<br />

across a semipermeable membrane of a certain pore size (Wienk, Marx, &<br />

Beynen, 1999). In fact, these methods measure only the amount of mineral<br />

available in the gastrointestinal tract for absorption, i.e., its so-called<br />

bioaccessibility (Salovaara, Sandberg, & Andlid 2002)). However, these<br />

methods are wi<strong>de</strong>ly used because of their good correlation with in vivo studies<br />

(Roig, Alegría, Barberá, Farré, & Lagarda, 1999; Wienk et al, 1999).<br />

Bioaccessibility values must be taken as relative in<strong>de</strong>xes for bioavailability,<br />

which means that the method used provi<strong>de</strong>s a good basis for establishing<br />

ten<strong>de</strong>ncies, comparisons and the <strong>de</strong>termination of effects caused by different<br />

factors (Azenha & Vascon<strong>de</strong>celos, 2000).<br />

In recent years, the mentioned in vitro methods have been used to<br />

estimate the BA of minerals from different foods (Roig et al, 1999; Jovaní,<br />

Alegría, Barberá, Farré, Lagarda, & Clemente, 2000; Sebastiá, Barberá, Farré,<br />

& Lagarda, 2001; Bosscher, Van Cauwenbergh, Van <strong>de</strong>r Auwera, Robberecht,<br />

& Deelstra, 2002; Sahuquillo, Barberá, & Farré, 2003;) and also from dishes<br />

and composite diets (Van Dyck et al. 1996; Pushpanjali & Khokhar, 1996;<br />

Luccarini, Canali, Cappelloni, Di Lullo & Lombardi-Boccia, 1999; Kennefick &<br />

Cashman, 2000).<br />

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