Universidad de Córdoba BIODISPONIBILIDAD MINERAL DE ...

Resultados
Standard Fe solutions were prepared immediately before use by dilution
of a standard solution of 1000mg Fe /L (Titrisol, Merck, Darmstadt, Germany).
All reagents used were of reagent grade and Millipore-Milli Q (Millipore
Ibérica S:A:, Barcelona, Spain) distilled-deionized water (DDW) was used
throughout.
Bathophenantroline (4,7-diphenyl-1,10-phenanthroline disulfonic acid)
(Sigma B-1375) was used. A solution was prepared by dissolving in DDW
12.5mg of bathophenantroline and 8.203 g of sodium acetate, after which the
volume was completed to 50 mL with DDW.
The reducing solution was prepared by dissolving 6.25g of
hydroxylamine hydrochloride (Aldrich, 15,941-7) in 25 mL of DDW. The
precipitation solution was prepared by mixing 10g trichloroacetic acid and 10
mL HCl (sp. gr 1.19) in 100 mL of DDW.
Contamination control: in order to avoid metal contamination glassware
was washed and rinsed with water and soaked in concentrated nitric acid
(sp.gr.1.40) for 15 min.. Then it was rinsed several times with DDW.
2.3. In vitro digestion: Solubility method
A modification of the method developed by Sauquillo (29) was used.
DDW (60mL) was added to 30g of meal, and the pH was adjusted to 2.0 with
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