390COMUNICAZIONI LIBEREtenziale di malignità (STUMP) e tumori maligni.Descriviamo un caso di neoplasia stromale prostatica, a prevalentedifferenziazione muscolare, che morfologicamenteinquadriamo nell’ ambito degli STUMP.Materiali e metodiPaziente di 75 anni che da 7 anni presentava pollachiuria,mitto ipovalido e stranguria. Ecografia: prostata aumentata divolume con calcolo vescicale. PSA: 0,22 ng/ml. All’interventochirurgico viene asportato un nodulo prostatico (diametrocm 5), ben delimitato, liscio, grigiastro, fascicolato eduro-elastico. La neoformazione, pseudocapsulata, è costituitada fasci di cellule fusate, variamente aggregate e con focaleaspetto storiforme. Le cellule hanno citoplasma eosinofiloa limiti indistinti e nucleo ovale o “a sigaro” con cromatinafinemente granulare. Rare e modeste le atipie cellulari. Rarissimele mitosi; necrosi assente. La neoplasia presenta vasidi diverso calibro e pochi linfociti talora aggregati in pseudofollicoli.Le ghiandole prostatiche, rare e normotipiche, sonostipate alla periferia della lesione. L’immunofenotipo tumoraleè il seguente: CD34+,vimentina+, desmina+, actina+,MNF116+ (focale), recettore progesterone+, S100-, Ki-67 70 anni 82 (25,3%) 44 (53,7%)Reperto rettalenegativo 204 (62,8%) 65 (31,9%) < 0,0001positivo 121 (37,2%) 68 (56,2%)PSA totale (ng/ml)< 4 22 (6,8%) 8 (36,4%) 0,114-10 220 (67,7%) 83 (37,7%)> 10 83 (25,5%) 42 (50,6%)Volume prostatico (cc)≤ 40 189 (58,2%) 96 (50,8%) < 0,0001> 40 136 (41,8%) 37 (27,2%)PSA density (ng/ml/cc)≤ 0,15 122 (37,5%) 28 (23%) < 0,0001> 0,15 203 (62,5%) 105 (51,7%)Density/TZ (ng/ml/cc)≤ 0,35 142 (46,1%) 29 (20,4%) < 0,0001> 0,35 166 (53,9%) 92 (54,4%)
PATOLOGIA URO-GENITALE391RisultatiÈ stata fatta diagnosi di adenocarcinoma della prostata in 134casi (41,2%), di ASAP in 19 casi (5,8%) e di PIN di alto gradoin 6 casi (1,8%). Il Gleason score (GS) è risultato pari a 5in un caso (0,7%), a 6 in 65 casi (48,5%), a 7 in 21 casi(15,6%), a 8 in 17 casi (12,6%), a 9 in 9 casi (6,7 %) e a 10in 4 casi (2,9%). In 17 casi (12,6%) il Gleason score non èstato assegnato a causa dell’esiguità dei microfocolai di adenocarcinomaosservati. In 166 casi l’esame istologico è risultatonegativo.La Tabella I stratifica la detection rate per le differenti variabilicliniche valutate.ConclusioniL’esecuzione di 14 biopsie prostatiche per via transperinealeconsente di diagnosticare un’elevata percentuale di neoplasiedella prostata. Il volume della ghiandola prostatica, il repertorettale e l’età dei pazienti influenzano in maniera significativala percentuale di positività registrate.Aurora B expression in normal testisand seminomasA. Caleo, G. Troncone, I. Migliaccio, A. Iaccarino, C.Frangella, M. Russo, F. Esposito, G. Portella * , P. Chieffi *Dipartimento di Scienze Biomorfologiche e Funzionali, Universitàdi Napoli ‘Federico II’; * Dipartimento di MedicinaSperimentale, II Università di NapoliIntroductionMitosis is a highly coordinated process that ensures the fidelityof chromosome segregation and is characterised bydramatic morphological changes which occur in a strictlysequential order. Serine/threonine protein kinase of the Aurorafamily (Aurora A/STK-15, Aurora B/AIM-1, AuroraC/AIK-3) are known to be required for the progressionthrough the M phase. Aurora B encodes a protein that associateswith condensing chromatin, concentrates at centromeres,and then relocates onto the central spindle atanaphase. High levels of Aurora kinases are characteristicof rapidly dividing cells and tumours. In particular, AuroraB has been found overexpressed in human cancer cells ofdifferent origin and in cell lines derived from colorectal tumours.Spermatogenesis is a hormonally regulated andunique developmental process whereby diploid stem cellsdifferentiate through an ordered sequence of steps and representsan ideal model for studying the control of cellulargrowth and differentiation.In this study the expression and the localisation of Aurora Bthroughout germinal epithelial progression in normal testisand its neoplastic counterpart were analysed.Methods and resultsImmunocytochemistry and RT-PCR analysis of mouse germinalepithelium cells showed the presence of Aurora B inspermatogonia and occasionally in spermatocytes. Westernblot analysis revealed the typical Aurora B isoform (41 kDa)in the same cellular types. A similar distribution was observedin human testis by immunohistochemistry. Moreover,the distribution and the expression of Aurora B were investigatedin neoplasms derived from germ cells. Surgical samplesof seminomas were analysed, and a high percentage ofAurora B positive cells (51%) was detected; the expressionof Aurora B was significantly related to the MIB-1 proliferationmarker (R = 0.816).ConclusionsThe data presented here demonstrate that Aurora B expressionoccurs in spermatogonial division. Furthermore, our resultsindicate that the expression of Aurora B is a consistentfeature of human seminomas. This observation is of clinicalinterest since Aurora B might be a target for cancer treatmentand may serve as a prognostic marker.ReferencesChieffi P, et al. J Endocrinol 2004;181(2):263-270.Aquaporin-1 in human varicocele testes, as acritical reabsorption factorP.A. Nicòtina * , G. Speciale * , S. Arena, C. Romeo, B. Zuccarello,F. Arena*Dipartimento di Patologia Umana; Dipartimento di ScienzePediatriche Mediche e Chirurgiche,Università di MessinaIntroductionVaricocele testes display exceeding endotubular fluid (ETF)and extracellular matrix (ECM), with subsequent oligoasthenozoospermia1 . Aquaporins (AQP s) are integral membraneproteins, modulating water transport across the cell membraneand reabsorptive exchanges. Previous experimental evidencewas provided that some AQP sare expressed in rat testis,as transmembrane water channels 2 . AQP-1, a major fluidtransporter, regulates tissue water flow and hydration. It hasbeen focally found in endothelial cells of human testes, buthas not been reported in endotubular cells. The present studywas aimed at immunolocalizing AQP-1 in human varicoceletestes.MethodsOpen testicular biopsies on 20 varicocele testes were comparedto 4 autoptical controls. Formalin-fixed, paraffin-embeddedsections were processed for histology. Immunohistochemistrywas performed using an AQP-1 monoclonal antibody,subsequent streptavidin-biotin/LSAB method and diaminobenzidinedevelopment. Positive immunoreactions were scoredas weak, moderate or strong.ResultsHistologically,disarranged tubular compartments, germ cellsloughing, ETF- and ECM-expancion were found. Cytoplasmicvacuoles in Sertoli cells, spermatogonia and spermatocytesalso occurred, with retained cytoplasmic droplets inthe spermatozoa, if present.AQP-1 immunostaining strongly depicted microvessel endothelialcells, as well as Sertoli and germ cells containingcytoplasmic vacuoles or retained droplets. Differently, 2 of 4control testes showed AQP-1 reactive endothelial cells only.ConclusionsThese results substantiate the common features of varicoceletestes, relating to oxidative membrane damages and defectiveETF- ECM-reabsorption.The immunostain patterns denote an AQP-1 overexpressionin endothelial cells, together with unexpectedly positive reactionsof Sertoli and germ cells. Such findings,that are lackingin the controls, suggesting a critical AQP-1 expression in varicoceletestes, to start a rapid pathway for water reabsorption,in both tubular and extratubular compartments.References1Zini A, et al. Fertil Steril 2000; 74: 461-464.2Badran HH, et al. J Androl 2002; 23: 358-373.
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