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MWCNTs can be collected by reversing the flow direction. The collecting process is shown in Figure 11. Figure 11. The collecting process for polystyrene microparticles and MWCNTs. (A) Particles injected in microfluidic channel. (B): DEP trapping when the electric field is applied on the electrodes. (C): Collection of polystyrene microparticles with the fluidic flow. (D): Releasing captured MWCNTs by switch off the applied electric field. (E): Collection of MWCNTs by reversing the flow direction. 5.5 More Discussions As in this work, all particles used for experiment were non-bioparticles, there are no limitations. However, causation should be experienced when the electric field stresses the live cells membrane [45]. Experimentally, strong electric field (large amplitude of alternating voltage) can generate large DEP force, which improves the separation efficiency. However, cells are damaged just in a short time after they are exposed to a very high electric field level [20] specially near the electrode tips. 108
The separation efficiency can be improved by optimising the system design. A multi-stage separation system could be one of the solutions and much work need to be conducted in order to minimise the detrimental effects of the applied electric field on live tissues. 6. Conclusion A platform for separating particles according to their dielectric response to alternating electric fields at specific frequencies has been presented. The performance of the device was simulated using CFD method. The dielectrophoretic spectrums for both polystyrene microparticles and MWCNTs were studied and compared to obtain the optimal frequency for particle separation. Experiments were carried out and MWCNTs were successfully separated from polystyrene microparticles for frequencies over 100 kHz. Particle behaviours at different frequencies were characterized and analysed. The developed device has potential novel applications such as: purifying cell suspensions with nanoscale impurities, tissues and organelles separation, as well as microelectronic and nanoelectronic device fabrications. Reference: [1] P. R. C. Gascoyne, and J. Vykoukal, “Particle separation by dielectrophoresis,” Electrophoresis, vol. 23, no. 13, pp. 1973-1983, Jul, 2002. [2] H. A. Pohl, Dielectrophoresis: The Behaviour of Neutral Matter in Nonuniform Elctric Field, Cambridge: Cambridge University Press, 1978. [3] A. T. J. Kadaksham, P. Singh, and N. Aubry, “Dielectrophoresis of nanoparticles,” Electrophoresis, vol. 25, no. 21-22, pp. 3625-3632, Nov, 2004. [4] N. Demierre, T. Braschler, R. Muller et al., “Focusing and continuous separation of cells in a microfluidic device using lateral dielectrophoresis,” Sensors and Actuators B-Chemical, vol. 132, no. 2, pp. 388-396, 2008. [5] E. M. Nascimento, N. Nogueira, T. Silva et al., “Dielectrophoretic sorting on a microfabricated flow cytometer: Label free separation of Babesia bovis infected erythrocytes,” Bioelectrochemistry, vol. 73, no. 2, pp. 123-128, 2008. [6] T. Yasukawa, M. Suzuki, T. Sekiya et al., “Flow sandwich-type immunoassay in microfluidic devices based on negative dielectrophoresis,” Biosensors & Bioelectronics, vol. 22, no. 11, pp. 2730-2736, May 15, 2007. [7] S. Grilli, and P. Ferraro, “Dielectrophoretic trapping of suspended particles by selective pyroelectric effect in lithium niobate crystals,” Applied Physics Letters, vol. 92, no. 23, 2008. [8] X. Xiong, A. Busnaina, S. Selvarasah et al., “Directed assembly of gold nanoparticle nanowires and networks for nanodevices,” Applied Physics Letters, vol. 91, no. 6, 2007. [9] A. Kuzyk, B. Yurke, J. J. Toppari et al., “Dielectrophoretic trapping of DNA origami,” Small, vol. 4, no. 4, pp. 447-450, 2008. [10] B. H. Lapizco-Encinas, S. Ozuna-Chacon, and M. Rito-Palomares, “Protein manipulation with insulator-based dielectrophoresis and direct current electric fields,” Journal of Chromatography A, vol. 1206, no. 1, pp. 45-51, Oct 3, 2008. [11] G. B. Salieb-Beugelaar, J. Teapal, J. van Nieuwkasteele et al., “Field-dependent DNA mobility in 20 nm high nanoslits,” Nano Letters, vol. 8, no. 7, pp. 1785-1790, Jul, 2008. [12] M. Yang, and X. Zhang, “Electrical assisted patterning of cardiac myocytes with controlled macroscopic anisotropy using a microfluidic dielectrophoresis chip,” Sensors and Actuators a- Physical, vol. 135, no. 1, pp. 73-79, 2007. [13] M. Suzuki, T. Yasukawa, H. Shiku et al., “Negative dielectrophoretic patterning with colloidal particles and encapsulation into a hydrogel,” Langmuir, vol. 23, no. 7, pp. 4088-4094, Mar 27, 2007. [14] A. Rosenthal, and J. Voldman, “Dielectrophoretic traps for single-particle patterning,” Biophysical Journal, vol. 88, no. 3, pp. 2193-2205, 2005. [15] E. T. Thostenson, Z. F. Ren, and T. W. Chou, “Advances in the science and technology of carbon nanotubes and their composites: a review,” Composites Science and Technology, vol. 61, no. 13, pp. 1899-1912, 2001. 109
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The 3 rd VACPS Research Workshop Pr
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Organizing Committee Victorian Asso
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Preface On the occasion of the publ
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Paper as a low-cost base material f
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Acknowledgements We gratefully ackn
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Career planning and Job Interview i
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The role of mitochondria in atrial
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Track-Before-Detect Procedures for
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3. Dynamic model for approaching ta
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Acknowledgments Xiaobo Deng would l
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Demonstration and Performance Analy
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Effect of mini-tyrosl-tRNA syntheta
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2.1 Materials Sprague-Dauley(SD) ma
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Distribution of three forms alpha-m
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Materials and Methods Ethics All an
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Fig.1 (A) Mass Spectrometry results
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indicated that there is more des-α
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Page 69 and 70: Investigation of the mechanism of t
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Page 76 and 77: cloud computing offers a cost-effec
Page 78 and 79: workflows. By comparing the generat
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Page 84 and 85: A Multi-modal Gesture Recognition S
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Page 101 and 102: Fig. 3 The optical and SEM microstr
Page 103 and 104: Conclusions (1) The large grains in
Page 105 and 106: [22-25]. Sorting of normal and Babe
Page 107 and 108: Figure 1. Г×Re[fCM] vs. frequency
Page 109 and 110: Figure 3. The DEP spectrum of cylin
Page 111 and 112: A B C Figure 5. The gradient of the
Page 113 and 114: Figure 7. SEM image of damaged elec
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Page 127 and 128: 2.8 Viability and morphology study
Page 129 and 130: Fig 3 and 4 SEM images of electrosp
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Page 135 and 136: well as on a usual nanofibrous scaf
Page 137 and 138: Uniform Coating of WO x on TiO 2 Na
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Page 150 and 151: Organizational Capabilities • Lea
Page 152 and 153: Table 1. Correlations, Reliabilitie
Page 154 and 155: [18]. Jap, S. D. (1999). Pie-expans
Page 156 and 157: Early Childhood Education Matters:
Page 158 and 159: decisions on selection of child car
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Page 162: An Exploration of Country of Origin
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Gas chromatographic retention indic
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Outward Foreign Direct Investment f
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Fiber Nonlinearity Precompensation
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Improved Nonlinearity precompensati
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