Workshop proceeding - final.pdf - Faculty of Information and ...

More documents

Recommendations

Info

phenomenon is also present between full-length TyrRS and mini-TyrRS. Researches have found a kind of angiogenesis regulatory factor, CXC chemotactic factor, but their function was dependent on whether or not they have the Glu-Leu-Arg (ELR) motif [45]. The CXC chemotactic factors which have the ELR motif could promote angiogenesis, while CXC chemotactic factors which do not have the ELR motif would be the antagonistic factors of angiogenisis. The situation is analogous to human mini-TyrRS and mini-TrpRS. Mini-TyrRS has an ELR motif and promotes angiogenesis, but mini- TrpRS does not have the ELR motif. Therefore, their function was totally opposite[46]. We were aware that angiogenic and angiostatic factors may work together to regulate angiogenesis. To test our hypothesis that mini-TyrRS (containing a natural ELR sequence) is an angiogenic factor, and that mini-TrpRS(containing a natural ELQ sequence) is an angiostatic factor, we evaluated whether mini-TyrRS/mini-TrpRS induced myocardial cells to format new capillaries using rats myocardial infarction model. We found out that irreversible ischemic injury areas was decreased and new capillaries formation was increased after mini-TyrRS administration by using TTC staining(Fig.3- 4) and the SP immunohistochemical method(Fig.5-6), but in mini-TrpRS group, myocardial infarction zone was increased and new capillaries formation was decreased after administration. In contrast, angiogenesis was not observed with full-length TyrRS and TrpRS. Interestingly, angiogenesis is stimulated by either mini-TyrRS or is inhibited by mini-TrpRS in rats myocardial infarction models, raising the possibility that mini-TyrRS/mini-TrpRS stimulates a common downstream signaling event. Thus, naturally occurring fragments of the two proteins involved in translation, TyrRS and TrpRS, have opposing activity on angiogenesis in the rat model. The opposing activities of the two tRNA synthetases suggest tight regulation of the balance between pro- and antiangiogenic stimuli, but the exact mechanism and relationship between them has not been clearly demonstrated. More research should be done to further explore the mechanism. References: 1.Losordo DW, Vale PR, Symes JF, Dunnington CH, Esakof DD, Maysky M, Ashare,AB Lathi K, Isner JM. Gene therapy for myocardial angiogenesis ;initial clinical results with direct myocardial injection of phVEGF165 as sole therapy for myocardial ischemia. Circulation 1998;98:2800-4. 2.Laham RJ, Sellke FW, Edelman JD, Pearlman JD, Ware A, Brown DL, Gold JP , Simons M. Local perivascular delivery of basic fibroblast growth factor in patients undergoing coronary bypass surgery :results of a phase I randomized, double-blind, placebo-controlled trial. Circulation 1999;100:1865-71. 3.Rosengart TK, Lee LY, Patel SR, Kligfield PD, Okin PM, Hackett NR, Isom OW , Crystal RG. Sixmonth assessment of a phase I trial of angiogenic gene therapy for the treatment of coronary artery disease using direct intramyocardial administration of an adenovirus vector expressing the VEGF121 cDNA. Ann Surg 1999;230,466-72. 4.Rosengart TK, Lee LY, Patel SR, Sanborn TA, Parikh M, Bergman GW, Hachamovitch R, Szulc M, Kligfield PD, Okin PM, Hahn RT, Devereux RB, Post MR, Hackett NR, Foster T, Grasso TM, Lesser ML, Isom OW, Crystal RG. Phase I assessment of direct intramyocardial administration of an adenovirus vector expressing the VEGF121 cDNA to individuals with clinically significant severe coronary artery disease. Circulation 1999;100:468-74. 5.Symes JF, Losordo DW, Vale PR, Lathi KG, Esakof DD, Maysky M, Isner JM. Gene therapy with vascular endothelial growth factor for inoperable coronary artery disease. Ann Thoracic Surg 1999;68:830-7. 6.Vale PR, Losordo DW, Milliken CE, McDonald MC, Gravelin LM, Curry CM, Esakof DD, Maysky M, Symes JF ,Isner JM. Randomized, singe-blind, placebo-controlled pilot study of catheter-based myocardiol gene transfer for therapeutic angiogenesis using left ventricular electromechanical mapping in patients with chronic myocardial ischemia. Circulation 2001;103:2138-43. 7.Pfeffer MA, Pfeffer JM, Fishbein MC, Fletcher PJ, Spadoro J, Kloner RA, Braunwald E. Myocardial infarct size and ventricular function in rats. Circulation Res 1979 ;44:503-12. 8.Michael LH, Entman ML, Hartley CJ, Youker KA, Zhu J, Hall SR, Hawkins HK, Berens K, Ballantyne CM. Myocardial ischemia and reperfusion : a murine model. Am J Physiol 1995;269:2147-54. 46
9.Du XJ, Gao X, Ramsey D. Surgical methods of inducing transverse aortic stenosis and myocardial infarction in the mouse. Asia Pacific Heart 1998;7:187-92. 10.Lutgens E, Daemen MJAP, de Muinck ED, Debet J, Leenders P, Smits JFM. Chronic myocardial infarction in the mouse :cardiac structural and functional changes. Cardiovascular Res 1999;41:586-93. 11.Michael LH, Ballantyne CM, Zachariah JP, Gould KE, Pocius JS, Taffet GE, Hartley CJ, Pham TT, Daniel SL, Funk E, Entman ML. Myocardial infarction and remodeling in mice. Effect of reperfusion. Am J Physiol 1999;277:660-8. 12.Scherrer-Crosbie M, Steudel W, Ullrich R, Hunziker PR, Liel-Cohen N, Newell J, Zaroff J, Zapol WM, Picard MH. Echocardiographic determination of risk area size in a murine model of myocardial ischemia. Am J Physiol 1999;277:986-92 13.Guo Y, Wu WJ, Qin Y ,Tang XL, Yang Z, Bolli R.Demonstration of an early and a late phase of ischemic preconditioning in mice. Am J Physiol 1998;275:1375-87. 14. Martinis SA, Plateau P, Cavarelli J, Florentz C .AminoacyltRNA synthetases: a family of expanding functions. EMBO J 1999;18:4591–6. 15.Wakasugi K, Schimmel P.Two distinct cytokines released from a human aminoacyl-tRNA synthetase. Science 1999;284:147–51. 16.Wakasugi K, Schimmel P.Highly differentiated motifs responsible for two cytokine activities of a split human tRNA synthetase. J Biol Chem 1999;274:23155–9. 17.Wakasugi K, Slike BM, Hood J, Ewalt KL, Cheresh DA, Schimmel P. Introduction of angiogenesis by a fragment of human tyrosyl-tRNA synthetase. J Biol Chem 2002;277:20124–6. 18.Wakasugi K, Slike BM, Hood J, Otani A, Ewalt KL, Friedlander M, Cheresh DA, Schimmel P.A human aminoacyl-tRNA synthetase as a regulator of angiogenesis. Proc Natl Acad Sci 2002;99:173–7. 19.Otani A, Slike BM, Dorrell M, Hood J, Kinder K, Ewalt KL, Cheresh D, Schimmel P, Friedlander M.A fragment of human TrpRS as a potent antagonist of ocular angiogenesis.Proc Natl Acad Sci USA 2002;99:178–83. 20.Ewalt KL, Schimmel P.Activation of angiogenic signaling pathways by two human tRNA synthetases. Biochemistry 2002;41:13344–9. 21.Ibba M.Aminoacyl-tRNA synthesis. Annu Rev Biochem 2000;69:617–50. 22.Ribas de Pouplana L, Frugier M, Quinn CL, Schimmel P.Evidence that two present-day components needed for the genetic code appeared after nucleated cells separated from eubacteria. Proc Natl Acad Sci USA 1996;93:166–70. 23.Lee SW, Cho BH, Park SG, Kim S.Aminoacyl-tRNA synthetase complexes: beyond translation. J Cell Sci 2004;117:3725–34. 24.Fleckner J, Martensen PM, Tolstrup AB, Kjeldgaard NO ,Justesen, J.Differential regulation of the human interferon inducible tryptophanyl-tRNA synthetase by various cytokines in cell lines. Cytokine 1995;7:70–7. 25.Kise Y, Lee SW, Park SG, Fukai S, Sengoku T, Ishii R, Yokoyama S, Kim S, Nureki O.A short peptide insertion crucial for angiostatic activity of human tryptophanyl-tRNA synthetase. Nat Struct Mol Biol 2004;11:149–56. 26.Jorgensen R, Sogaard TM, Rossing AB, Martensen PM, Justesen J.Identification and characterization of human mitochondrial tryptophanyl-tRNA synthetase. J Biol Chem 2000;275:16820–6. 27.Schaper W, De Bradbander M, Lewi P. DNA synthesis and mitoses in coronary collateral vessels of the dog. Circulation Res 1970;28:671-9. 28.Shou M, Thirumurti V,Sharnini Rajanayagam MA, Lazarous DF, Hodge E, Stiber JA, Pettiford M, Elliot E, Shah SM, Unger EF. Effect of basic fibroblast growth factor on myocardial angiogenesis in dogs with mature collateral vessels.J Am College Cardiogl 1997;29:1102-6. 29.Mannion JD, Blood V, Bailey W ,Bauer TL, Magno MG, Dimeo F,Epple A, Spinale FG.The effect of basic fibroblast growth factor on the blood flow and morphologic features of a latissimus dorsi cardiomyoplasty. J Thoracic Cardiovascular Surg 1996;111:19-28. 30.Ware JA, Simons M. Angiogenesis and cardiovascular disease. Oxford university press, 1999,New York. 47
Page 2 and 3:
The 3 rd VACPS Research Workshop Pr
Page 4 and 5: Organizing Committee Victorian Asso
Page 6 and 7: Preface On the occasion of the publ
Page 8 and 9: Paper as a low-cost base material f
Page 10 and 11: Acknowledgements We gratefully ackn
Page 13 and 14: Career planning and Job Interview i
Page 15 and 16: The role of mitochondria in atrial
Page 18 and 19: Track-Before-Detect Procedures for
Page 20 and 21: 3. Dynamic model for approaching ta
Page 22 and 23: ⎧π0 if j = i and Cx ij k > δij
Page 24 and 25: k k k k ( ) p( p Ζ | X , B = ∏ z
Page 26 and 27: As illustrated in Fig.4, the struct
Page 28 and 29: ( xˆ , m ˆ ) = argmax Γ ( x , m
Page 30 and 31: (a) (b) Fig. 7. mesh plot of the re
Page 32 and 33: Definition 2 The probability of tar
Page 34 and 35: Acknowledgments Xiaobo Deng would l
Page 36 and 37: speed, several methods can be utili
Page 38 and 39: Where u ′ , v′ ) is the point p
Page 40 and 41: ase on SIFT are also unsuitable to
Page 42 and 43: Demonstration and Performance Analy
Page 45 and 46: Effect of mini-tyrosl-tRNA syntheta
Page 47 and 48: 2.1 Materials Sprague-Dauley(SD) ma
Page 49 and 50: Fig. 2. The representative photomic
Page 51 and 52: mount/0.1mm 2 , as for mini-TrpRS g
Page 53: 3.4.The mRNA expression of mini-Tyr
Page 57 and 58: Distribution of three forms alpha-m
Page 59 and 60: Materials and Methods Ethics All an
Page 61 and 62: Fig.1 (A) Mass Spectrometry results
Page 63 and 64: indicated that there is more des-α
Page 65 and 66: Fig.6 (A) Mass Spectrometry results
Page 67 and 68: transcription-polymerase chain reac
Page 69 and 70: Investigation of the mechanism of t
Page 71 and 72: Dual mode roll-up effect in a multi
Page 73: Paper as a low-cost base material f
Page 76 and 77: cloud computing offers a cost-effec
Page 78 and 79: workflows. By comparing the generat
Page 80 and 81: [10] E. Deelman, G. Singh, M. Livny
Page 82 and 83: Web Page Prediction Based on Condit
Page 84 and 85: A Multi-modal Gesture Recognition S
Page 86: Searching for Fair Joint Gains in A
Page 89 and 90: environments. However, challenges a
Page 91 and 92: conclude that the hydrophobic PHB c
Page 93 and 94: Figure 4. (a) Energy transfer model
Page 95 and 96: 20 Intensity (/10 6 ) / a.u. 15 10
Page 97 and 98: (22) (a) Cho, J.; Quinn, J. F.; Car
Page 99 and 100: Grain refinement of pure magnesium
Page 101 and 102: Fig. 3 The optical and SEM microstr
Page 103 and 104: Conclusions (1) The large grains in
Page 105 and 106:
[22-25]. Sorting of normal and Babe
Page 107 and 108:
Figure 1. Г×Re[fCM] vs. frequency
Page 109 and 110:
Figure 3. The DEP spectrum of cylin
Page 111 and 112:
A B C Figure 5. The gradient of the
Page 113 and 114:
Figure 7. SEM image of damaged elec
Page 115 and 116:
Figure 9. Particle separation progr
Page 117 and 118:
The separation efficiency can be im
Page 119 and 120:
[37] A. Motayed, M. Q. He, A. V. Da
Page 121 and 122:
Fig. 1. The transverse and longitud
Page 123 and 124:
References: [1] Xiong Liu, Mark C.
Page 125 and 126:
new tissue[10]. Most of these human
Page 127 and 128:
2.8 Viability and morphology study
Page 129 and 130:
Fig 3 and 4 SEM images of electrosp
Page 131 and 132:
studies have indicated that surface
Page 133 and 134:
Fig 10 Tensile Stress curve of elec
Page 135 and 136:
well as on a usual nanofibrous scaf
Page 137 and 138:
Uniform Coating of WO x on TiO 2 Na
Page 140 and 141:
Union as a Social Regulator of Mark
Page 142 and 143:
associated with a reinvention of in
Page 144 and 145:
enterprises…There obviously is no
Page 146 and 147:
Union assistants participate in org
Page 148 and 149:
[5] Deyo, Frederic C and Ağartan,
Page 150 and 151:
Organizational Capabilities • Lea
Page 152 and 153:
Table 1. Correlations, Reliabilitie
Page 154 and 155:
[18]. Jap, S. D. (1999). Pie-expans
Page 156 and 157:
Early Childhood Education Matters:
Page 158 and 159:
decisions on selection of child car
Page 160 and 161:
Noble, K. (2007). Parent choice of
Page 162:
An Exploration of Country of Origin
Page 165 and 166:
Supercontinuum generation for fiber
Page 167 and 168:
Gas chromatographic retention indic
Page 169 and 170:
Outward Foreign Direct Investment f
Page 171 and 172:
Fiber Nonlinearity Precompensation
Page 173 and 174:
Improved Nonlinearity precompensati
show all

Workshop proceeding - final.pdf - Faculty of Information and ...

Create successful ePaper yourself

Delete template?

Save as template?