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Efficient Ethanol production in glucose/xylose co-fermentations by a novel co-culture scheme of Zymomonas mobilis and Pichia stipitis Nan Fu 1 * , Paul Peiris 1 , Julie Markham 1 , John Bavor 1 * Presenter 1. School of Natural Sciences, University of Western Sydney, Locked Bag 1797, Penrith South DC, NSW 1797, Australia The efficiency of bioethanol production from lignocellulose would be significantly improved if the glucose and xylose in the hydrolysate could be simultaneously converted to ethanol. This study designed a novel fermentation scheme, using a glucose-fermenting strain, Zymomonas mobilis and a pentose utilizing strain, Pichia stipitis to convert the sugar mixture substrates including both synthetic media and hydrolysate media. Fermentation characteristics of the two organisms were optimized in media containing 50 g/l of the corresponding sugars. The inoculum was a key factor in the optimization for the production of ethanol. A ten fold increase in the cell number in the inoculum of Z. mobilis reduced the fermentation time from 8 to 2.5 h, resulting in an improvement in the overall volumetric ethanol productivity from 2.93 to 10.54 g/l/h. Similarly, a five fold increase in the cell number in the inoculum of P. stipitis resulted in a decrease of the fermentation time from 130 to 28 h, leading to an increase of the volumetric productivity from 0.14 to 0.73 g/l/h. This is the highest value of volumetric ethanol productivity for Pichia stipitis ever reported. Co-fermentation of sugar mixture simulating the bagasse hydrolysate (30 g/l glucose and 20 g/l xylose) was carried out with different fermentation schemes which included free cells, immobilized cells, co-culture and sequential culture. Co-culture with immobilized Z. mobilis and free cells of P. stipitis proved to be the best scheme. Complete utilization of the sugars within 19 h with an ethanol yield of 0.49 g/g was achieved, giving a volumetric productivity of 1.277 g/l/h. Application of this fermentation scheme on sugarcane bagasse hydrolysate resulted in a complete sugar utilization within 26 h; ethanol yield peaked at 40 h with a yield of 0.49 g/g. These results are the highest reported values for co-culture and compare favorably with best results reported with recombinant strains. 62
Dual mode roll-up effect in a multicomponent near adiabatic adsorption process Gang Li, 1 * Dong Xu, 1,2 Penny Xiao, 1 Paul A Webley 1 * Presenter 1. Cooperative Research Centre for Greenhouse Gas Technologies, Department of Chemical Engineering, Monash University, Wellington Road, Clayton, Victoria 3800, Australia 2. School of Materials & Metallurgy, Northeastern University, Shenyang 110004, P.R. China Adsorption technologies have been widely used in laboratorial analysis/separations, e.g. chromatography and portable life support systems, and also in industrial process, e.g. air separation and carbon capture. A common phenomenon in multi-component adsorption in a fixed bed is that the effluent concentration of some components may exceed their steady inlet values. This effect, often referred to as roll-up, is attributed to the displacement of the more weakly adsorbed component by the slower moving more strongly adsorbed component, according to the conventional explanation in the literature. This has also been applied to some practical operations in liquid chromatography and some large-scale chromatographic separations to desorb the pre-loaded components from a column by introducing a desorbent, which is more strongly adsorbed than any other component in the system. Many published works reported that in a system containing two adsorbed species, one could easily see a single roll-up of the weak component during the breakthrough process. However, our recent study showed that more than one roll-up could occur, in such a system using CO 2 and H 2 O vapour as the weakly and strongly adsorbed components respectively in double zeolite 3A/13X and activated alumina/13X layered columns. It has also been found that a pure thermal wave was generated by H 2 O adsorption and it moved ahead of the H 2 O mass transfer zone, and the first roll-up coincided with the propagation of the thermal front to the 3A/13X layer interface while the second roll-up happened when H 2 O concentration front reached the 13X layer. In theory, enthalpy can be regarded as a direct equivalence to an extra adsorbed component so that temperature front will sweep off the column before the propagation of the strongly adsorbed component (H 2 O in this case), but it only signifies under adiabatic or near adiabatic conditions. The interval of the two roll-ups reduces with the decrease of the length of 3A layer, and eventually they merge into a single pronounced roll-up. The results were further verified by computer simulations. Therefore, to the best of our knowledge, this is the first affirmative report about this unique dual mode roll-up effect, which will be essential in the design of near adiabatic adsorption/chromatography units. 63
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The 3 rd VACPS Research Workshop Pr
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Organizing Committee Victorian Asso
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Preface On the occasion of the publ
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Paper as a low-cost base material f
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Acknowledgements We gratefully ackn
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Career planning and Job Interview i
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The role of mitochondria in atrial
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Track-Before-Detect Procedures for
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Page 84 and 85: A Multi-modal Gesture Recognition S
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Fig. 1. The transverse and longitud
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References: [1] Xiong Liu, Mark C.
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new tissue[10]. Most of these human
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2.8 Viability and morphology study
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Fig 3 and 4 SEM images of electrosp
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studies have indicated that surface
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Fig 10 Tensile Stress curve of elec
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well as on a usual nanofibrous scaf
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Uniform Coating of WO x on TiO 2 Na
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Union as a Social Regulator of Mark
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associated with a reinvention of in
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enterprises…There obviously is no
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Union assistants participate in org
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[5] Deyo, Frederic C and Ağartan,
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Organizational Capabilities • Lea
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Table 1. Correlations, Reliabilitie
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[18]. Jap, S. D. (1999). Pie-expans
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Early Childhood Education Matters:
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decisions on selection of child car
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Noble, K. (2007). Parent choice of
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An Exploration of Country of Origin
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Supercontinuum generation for fiber
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Gas chromatographic retention indic
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Outward Foreign Direct Investment f
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Fiber Nonlinearity Precompensation
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Improved Nonlinearity precompensati
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