Workshop proceeding - final.pdf - Faculty of Information and ...
Workshop proceeding - final.pdf - Faculty of Information and ...
Workshop proceeding - final.pdf - Faculty of Information and ...
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3.4.The mRNA expression <strong>of</strong> mini-TyrRS / mini-TrpRS at infarction marginal zone<br />
RT-PCR was used to detect the mRNA expression <strong>of</strong> mini-TyrRS/mini-TrpRS at infarction zone.<br />
The results indicated that: Compared with sham group, the expression level <strong>of</strong> CAL was increase <strong>and</strong><br />
had significantly different in mini-TyrRS group(P < 0.05 or P < 0.01), after administration <strong>of</strong> mini-<br />
TyrRS, there was a significant increase in expression level compared with the CAL group <strong>and</strong> sham<br />
group.(P < 0.05 or P < 0.01).But the expression level between sham-operated <strong>and</strong> CAL groups was<br />
not significantly different in mini-TrpRS group(p>0.05), after administration <strong>of</strong> mini-TrpRS, there was<br />
a significant increase in expression level compared with the CAL group <strong>and</strong> sham group.(P < 0.05 or<br />
P < 0.01).see Fig.7-8.<br />
Fig.8 Mini-TrpRS mRNA expression in infarcted left ventricular tissue in CAL rats at different time<br />
points. (A) mRNA <strong>of</strong> mini- TrpRS at 3 days;(B) mRNA <strong>of</strong> mini- TrpRS at 7 days; (C) mRNA <strong>of</strong> mini-<br />
TrpRS at 14 days; (D) mRNA <strong>of</strong> mini- TrpRS at 28 days. Sham, sham-operation; CAL, coronary<br />
artery ligation; CAL + mini- TrpRS (20μl, twice daily, 600μg. Kg -1 .day -1 ). Lane 1, Sham; Lane 2,<br />
CAL; Lane 3, CAL + mini- TrpRS. Data are shown as the means±S.D. (n = 5 for each time point). * P<br />
< 0.05,**P < 0.01 vs. sham operation group; #P < 0.05, ##P < 0.01 vs. CAL group.<br />
4. Discussion<br />
Preclinical models <strong>of</strong> myocardial ischaemia have been reported in several large animals species,<br />
including dogs[27-28]<strong>and</strong> goats [29].The model that most closely resembles the response seen in<br />
humans is the pig ameroid model[30],which has been used in a variety <strong>of</strong> therapeutic studies[31-<br />
39].However, the expense <strong>and</strong> practical dem<strong>and</strong>s <strong>of</strong> porcine surgical facilities severely limit the extent<br />
<strong>of</strong> such studies,precluding the use <strong>of</strong> this model for large scale screening studies <strong>of</strong> novel therapeutic<br />
approaches.<br />
In normal cells, human TrpRS exists as a full length form <strong>and</strong> as a truncated form designated mini-<br />
TrpRS, which is produced by alternative splicing[40]. Expression <strong>of</strong> mini-TrpRS is highly stimulated<br />
in human cells by the addition <strong>of</strong> IFN-c [41-43]. Although both human full-length TrpRS <strong>and</strong> mini-<br />
TrpRS are enzymatically active in aminoacylation, they differ in angiostatic activity[23; 44]. The same<br />
45