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31.Giordano FJ, Ping P, McKirnan D,Nozaki S, DeMaria AN, Dillmann WH, Mathieu CO, Hammond K. Intracoronary gene transfer of fibroblast growth factor-5 increases blood flow and contractile function in an ischemic region of the heart. Nature Med 1996;2:534-9. 32.Hariawala MD, Horowitz JR, Esakof D, Sheriff DD, Walter DH, Keyt B, Isner J, Symes JF.VEGF improves myocardial blood flow but produces EDRF-Mediated hypotension in porcine hearts. J Surg Res 1996;63:77-82. 33.Lopez JJ, Laham RJ, Stamler A, Pearlman JD, Bunting S, Kaplan A, Carrozza JP, Sellke FW.VEGF administration in chronic myocardial ischemia in pigs. Cardiovascular Res 1998;40:272-81. 34.Mack CA, Patel SR, Schwarz EA, Zanzonico P, Hahn RT, Ilercil A, Devereux RB, Goldsmith SJ, Christian TF, Sanborn TA, Kovesdi I, Hackett N, Isom OW, Crystal RG, Rosengart TK. Biologic bypass with the use of adenovirus-mediated gene transfer of the complementary deoxyribonucleic acid for vascular endothelial growth factor 121 improves myocardial perfusion and function in the ischemic porcine heart. J Thoracic Cardiovascular Surg 1998;115:168-77. 35.Tio RA, Tkebuchava T, Scheuermann TH, Lebherz C, Magner M, Kearny M, Esakof DD, Isner JM, Symes JF. Intramyocardial gene therapy with naked DNA encoding vascular endothelial growth factor improves collateral flow to ischemic myocardium. Huamn Gene Ther 1999;10:2953-60. 36.Patel SR, Lee LY, Mack CA,Polce DR, El-Sawy T, Hackett NR, Ilercil A, Jones EC, Hahn RT, Isom OW, Rosengart TK, Crystal RG.Safety of direct myocardial administration of an adenovirus vector encoding vascular endothelial growth factor 121 .Human Gene Ther 1999;10:1331-48. 37.Laham RJ, Rezaee M, Post M, Novicki D, Sellke FW, Pearlman JD, Simons M, Hung D.Intrapericardial delivery of fibroblast growth factor-2 induces neovascularization in a porcine model of chronic myocardial ischaemia. J Pharmacol Exp Therapeutics 2000;292:795-802. 38.Sato K, Laham RJ, Pearlman JD, Novicki D, Sellke FW, Simons M, Post MJ. Efficacy of intracoronary versus intravenous FGF-2 in a pig model of chronic myocardial ischemia. Ann Thoracic Surg 2000;70:2113-8. 39.Sato K, Wu T, Laham RJ, Johnson RB, Douglas P, Li J, Sellke FW, Bunting S, Simons M, Post MJ. Efficacy of intracoronary or intravenous VEGF in a pig model of chronic myocardial ischemia. J Am College Cardiol 2001;37:616-23. 40.Yeh CH, Peng HC, Huang TF.Accutin, a new disintegrin,inhibits angiogenesis in vitro and in vivo by acting as integrin alphavbeta-antagonist and inducing apoptosisl.J Blood 1998;92:3268–76 41.Tolstrup AB, Bejder A, Fleckner J, Justesen J.Transcriptional regulation of the interferon-c inducible tryptophanyltRNA synthetase includes alternative splicing. J Biol Chem 1995;270:397– 403. 42.Fleckner J, Rasmussen HH, Justesen J.Human interferon gamma potently induces the synthesis of a 552 kDa protein highly homologous to rabbit peptide chain release factor and bovine tryptophan. Proc Natl Acad Sci 1991;88:11520–4. 43.Shaw AC, Rossel LM, Roepstorff P, Justesen J, Christiansen G, Birkelund S. Mapping and identification of interferon gamma-regulated HeLa cell proteins separated by immobilized pH gradient two dimensional gel electrophoresis. Electrophoresis 1999;20:984–93. 44.Tzima E, Reader JS, Irani-Tehrani M, Ewalt KL, Schwartz MA, Schimmel P. VE-cadherin links tRNA synthetase cytokine to anti-angiogenic function. J Biol Chem 2005;280:2405–8 45. Clark LI, Dewald B, Geiser T ,Moser B, Baggiolini M.Platelet factor 4 binds to interleukin 8 receptors and activates neutrophils when its N terminus is modified with Glu-Leu-Arg. Proc Natl Acad Sci 1993;90:3574–7. 46.Jeong EJ, Hwang GS, Kim KH, Kim MJ, Kim S, Kim KS.Structural analysis of multi-functional peptide motifs present in human bifunctional tRNA synthetase: identification of RNA binding residues and functional implications for tandem repeats. Biochemistry 2000;39:15775–82. 48
Distribution of three forms alpha-melanocyte stimulating hormone in the brain of male and female sheep by using HPLC and Mass Spectrometry Analysis Rui Zeng 1,2 * , A.Ian.Smith 2 , Iain.J.Clarke 3 * Presenter 1. Department of Cardiovascular diseases, West China Hospital, School of Clinic Medicine, Sichuan University, Chengdu 610041, China 2. Department of Biochemistry & Molecular Biology, Faculty of Medicine, Nursing and Health Sciences, Monash University, Vic 3800,Australia 3. Department of Physiology, Faculty of Medicine, Nursing and Health Sciences, Monash University, Vic 3800, Australia To compare des-acetyl α-MSH(des-α-MSH), acetyl α-MSH(act-α-MSH) and di-acetyl α- MSH(di-act-α-MSH) between the male and female sheep in ten special brain tissues. All of this will let us ascertain whether different gonadal steroids causes any change in acetylated peptide. Mass spectrometry (MS) was used for molecular weight determination, HPLC/EIA was also used for quantitation. Before MS, Immunomagnetic protein isolation using Dynabeads Protein A also provides a fast and reliable method for capturing Ig for small scale purification. Mass spectrometry analysis indicated that: In female sheep, all three forms of α- MSH were detected in hypothalamus, neurointermediate lobe, cortex, pre-optic area and median eminence. In arcuate nucleus, des- and act-α-MSH were present, and only acetylated forms(including mono-act and di-act) were detected in anterior pituitary. For male sheep, only in neurointermediate lobe and pre-optic area were present all three forms of α-MSH. In hypothalamus, median eminence, cortex, anterior pituitary, des- and act-α-MSH were detected. We can only find des-α-MSH in arcuate nucleus. Cerebellum, spinal cord and brain stem have no any forms of α-MSH both in male and female sheep. HPLC/EIA results showed that in hypothalamus, neurointermediate lobe, pre-optic area and median eminence, there is no any difference in des-α-MSH between male and female sheep, but female sheep has more act-α- MSH and di-act-α-MSH compared with male sheep.P < 0.05. In arcuate nucleus, there is no any difference in des-α-MSH between male and female sheep, but female sheep has more actα-MSH only compared with male sheep.P < 0.05. In anterior pituitary, there is more des-α- MSH in male sheep, but female sheep has more act-α-MSH and di-act-α-MSH compared with male sheep. P < 0.05. Finally, in cerebral cortex, female sheep has more des-α-MSH, act-α- MSH and di-act-α-MSH compared with male sheep.P < 0.05. These data suggest that N- acetylation of products formed by the processing of α-MSH can markedly adapt to their biological properties. The results also illustrated that the distribution of α-MSH may be affected by the sex steroid hormone. Keywords α-MSH, POMC, sheep, HPLC, Mass Spectrometry Introduction Proopiomelanocortin (POMC) is a precursor protein that contains the sequence for several bioactive peptides including adrenocorticotropin (ACTH), β-endorphin(β-EP), and melanocytestimulating hormone (α-, β- and γ- MSH). The functional peptides have been detected in a variety of tissues. Post-translational processing of prohormones is an important biochemical mechanism employed by many secretory cells to generate a diversity of biological peptides from the same gene product, a process that occurs in a tissue-specific manner (1). Similarly to many prohormones, POMC is 49
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The 3 rd VACPS Research Workshop Pr
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Organizing Committee Victorian Asso
Page 6 and 7: Preface On the occasion of the publ
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Page 10 and 11: Acknowledgements We gratefully ackn
Page 13 and 14: Career planning and Job Interview i
Page 15 and 16: The role of mitochondria in atrial
Page 18 and 19: Track-Before-Detect Procedures for
Page 20 and 21: 3. Dynamic model for approaching ta
Page 22 and 23: ⎧π0 if j = i and Cx ij k > δij
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Page 36 and 37: speed, several methods can be utili
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Page 40 and 41: ase on SIFT are also unsuitable to
Page 42 and 43: Demonstration and Performance Analy
Page 45 and 46: Effect of mini-tyrosl-tRNA syntheta
Page 47 and 48: 2.1 Materials Sprague-Dauley(SD) ma
Page 49 and 50: Fig. 2. The representative photomic
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Page 53 and 54: 3.4.The mRNA expression of mini-Tyr
Page 55: 9.Du XJ, Gao X, Ramsey D. Surgical
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Page 61 and 62: Fig.1 (A) Mass Spectrometry results
Page 63 and 64: indicated that there is more des-α
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Page 69 and 70: Investigation of the mechanism of t
Page 71 and 72: Dual mode roll-up effect in a multi
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Page 76 and 77: cloud computing offers a cost-effec
Page 78 and 79: workflows. By comparing the generat
Page 80 and 81: [10] E. Deelman, G. Singh, M. Livny
Page 82 and 83: Web Page Prediction Based on Condit
Page 84 and 85: A Multi-modal Gesture Recognition S
Page 86: Searching for Fair Joint Gains in A
Page 89 and 90: environments. However, challenges a
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Page 93 and 94: Figure 4. (a) Energy transfer model
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Page 99 and 100: Grain refinement of pure magnesium
Page 101 and 102: Fig. 3 The optical and SEM microstr
Page 103 and 104: Conclusions (1) The large grains in
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Figure 1. Г×Re[fCM] vs. frequency
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Figure 3. The DEP spectrum of cylin
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A B C Figure 5. The gradient of the
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Figure 7. SEM image of damaged elec
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Figure 9. Particle separation progr
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The separation efficiency can be im
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[37] A. Motayed, M. Q. He, A. V. Da
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Fig. 1. The transverse and longitud
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References: [1] Xiong Liu, Mark C.
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new tissue[10]. Most of these human
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2.8 Viability and morphology study
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Fig 3 and 4 SEM images of electrosp
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studies have indicated that surface
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Fig 10 Tensile Stress curve of elec
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well as on a usual nanofibrous scaf
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Uniform Coating of WO x on TiO 2 Na
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Union as a Social Regulator of Mark
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associated with a reinvention of in
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enterprises…There obviously is no
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Union assistants participate in org
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[5] Deyo, Frederic C and Ağartan,
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Organizational Capabilities • Lea
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Table 1. Correlations, Reliabilitie
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[18]. Jap, S. D. (1999). Pie-expans
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Early Childhood Education Matters:
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decisions on selection of child car
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Noble, K. (2007). Parent choice of
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An Exploration of Country of Origin
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Supercontinuum generation for fiber
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Gas chromatographic retention indic
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Outward Foreign Direct Investment f
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Fiber Nonlinearity Precompensation
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Improved Nonlinearity precompensati
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