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Fig.6 Angiogenesis (capillary density, measured at day 3, 7, 14 and 28 after ligation) was inhibited by mini-TrpRS and augmented by mini-TyrRS, while sham had no angiogenesis effect compared with CAL. Data are shown as the means±S.D. (n = 5 for each time point, each group). #P < 0.05, ##P < 0.01 vs. CAL group. Fig.7 Mini-TyrRS mRNA expression in infarcted left ventricular tissue in CAL rats at different time points. (A) mRNA of mini-TyrRS at 3 days;(B) mRNA of mini-TyrRS at 7 days; (C) mRNA of mini- TyrRS at 14 days; (D) mRNA of mini-TyrRS at 28 days. Sham, sham-operation; CAL, coronary artery ligation; CAL + mini-TyrRS (20μl, twice daily, 600μg. Kg -1 .day -1 ). Lane 1, Sham; Lane 2, CAL; Lane 3, CAL + mini-TyrRS. Data are shown as the means±S.D. (n = 5 for each time point, each group). * P < 0.05,**P < 0.01 vs. sham operation group; #P < 0.05, ##P < 0.01 vs. CAL group. 44
3.4.The mRNA expression of mini-TyrRS / mini-TrpRS at infarction marginal zone RT-PCR was used to detect the mRNA expression of mini-TyrRS/mini-TrpRS at infarction zone. The results indicated that: Compared with sham group, the expression level of CAL was increase and had significantly different in mini-TyrRS group(P < 0.05 or P < 0.01), after administration of mini- TyrRS, there was a significant increase in expression level compared with the CAL group and sham group.(P < 0.05 or P < 0.01).But the expression level between sham-operated and CAL groups was not significantly different in mini-TrpRS group(p>0.05), after administration of mini-TrpRS, there was a significant increase in expression level compared with the CAL group and sham group.(P < 0.05 or P < 0.01).see Fig.7-8. Fig.8 Mini-TrpRS mRNA expression in infarcted left ventricular tissue in CAL rats at different time points. (A) mRNA of mini- TrpRS at 3 days;(B) mRNA of mini- TrpRS at 7 days; (C) mRNA of mini- TrpRS at 14 days; (D) mRNA of mini- TrpRS at 28 days. Sham, sham-operation; CAL, coronary artery ligation; CAL + mini- TrpRS (20μl, twice daily, 600μg. Kg -1 .day -1 ). Lane 1, Sham; Lane 2, CAL; Lane 3, CAL + mini- TrpRS. Data are shown as the means±S.D. (n = 5 for each time point). * P < 0.05,**P < 0.01 vs. sham operation group; #P < 0.05, ##P < 0.01 vs. CAL group. 4. Discussion Preclinical models of myocardial ischaemia have been reported in several large animals species, including dogs[27-28]and goats [29].The model that most closely resembles the response seen in humans is the pig ameroid model[30],which has been used in a variety of therapeutic studies[31- 39].However, the expense and practical demands of porcine surgical facilities severely limit the extent of such studies,precluding the use of this model for large scale screening studies of novel therapeutic approaches. In normal cells, human TrpRS exists as a full length form and as a truncated form designated mini- TrpRS, which is produced by alternative splicing[40]. Expression of mini-TrpRS is highly stimulated in human cells by the addition of IFN-c [41-43]. Although both human full-length TrpRS and mini- TrpRS are enzymatically active in aminoacylation, they differ in angiostatic activity[23; 44]. The same 45
Page 2 and 3: The 3 rd VACPS Research Workshop Pr
Page 4 and 5: Organizing Committee Victorian Asso
Page 6 and 7: Preface On the occasion of the publ
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Fig 3 and 4 SEM images of electrosp
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Fiber Nonlinearity Precompensation
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