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triphenyltetrazolium chloride (TTC) solution (1%) for 30 min in phosphate buffer at 37℃. Sections were fixed overnight in 4% paraformaldehyde for contrast enhancement between stained and unstained tissue. TTC stained the viable tissue with red, while the necrotic tissue remained discolored. The sections were then placed between two cover slips and digitally photographed using a Nikon coolpix S10 camera, and quantified with the weight respectively. The area of irreversible injury (TTC negative) is presented as a percentage of the area(the irreversible injury area/the total weight of ventricles). 2.7.Measured the density of capillary The streptavidin peroxidase (SP) immunohistochemical method was used to detect the expression of factor VIII in myocardial infarction margin areas. The dilution of factor VIII rat monoclonal antibody (Santa Cruz, CA, USA) was 1:100. The procedure was performed according to the manufacturer’s instructions.The positive cells were identified, counted and analyzed under the inverted phase-contrast microscope (Olympus, Japan) in 3 different fields(0.1mm 2 ) at 400 magnification using Image-proplus 6.0 software.Results were normalized by arbitrarily setting the total tube area of control to 100%. 2.8. Real time fluorescent quantitation PCR (RT-PCR) After ligation, myocardial tissues were harvested at the times indicated, washed twice with icecold phosphate-buffered saline (PBS) and collected by centrifugation. Total RNA was isolated using the Trizol reagent (MRC, USA) according to the manufacturer's instructions. Total RNA (5 µL) was converted to complementary DNA (cDNA) using Revert Aid First Strand cDNA Synthesis Kit. A 5µL aliquot of the resulting cDNA was used as template for PCR amplification with the following primers: mini-TrpRS, P1 (forward, 5’- CCC TGC TGC ACT CCA CCT T-3’), P2 (reverse, 5’- ACG CAT GCT TAT TGA CCT TG-3’); mini- TyrRS, P3 (forward, 5’- CAT CTG ATG AAT CCT ATG GTT -3’), P4 (reverse, 5’- GGA TCA CAA ACT CGG ACT TA-3’); β-actin, P5 (forward, 5’-GCC AAC ACA GTG CTG TCT -3’), P6 (reverse, 5’-AGG AGC AAT GAT CTT GAT CTT -3’). The amplifications were performed by an initial denaturation (94 °C for 2 min), followed by 45 cycles of denaturation, annealing and extension (94 °C for 20 s, 54 °C for 20 s, 72 °C for 30 s), and a final extension (72 °C for 5 min). The transcript of β-actin was also amplified by RT-PCR from the same cDNA template and was used as an internal control. All the primers were designed and synthesized by Genepharma (Shanghai, China). The identity of each PCR product was confirmed by DNA sequencing. The pictures were scanned and analyzed by the Gel Doc 1000 gel imaging system. 2.9.Statistical analysis Results are expressed as mean ± standard deviation. Comparison of means was performed by means of the analysis of variance procedure (Student–Newman–keuls test, SPSS 13.0 for Windows). P
Fig. 2. The representative photomicrographs of neoformative blood capillaries in infarcted LV area, stained with hematoxylin and eosin. Black arrowheads point to newly formed blood vessels and neoformative blood capillary at different time point. Sham, sham operation; CAL, coronary artery ligation; CAL + mini-TyrRS, mini-TyrRS (20μl, twice daily, 600μg. Kg -1 .day -1 ); CAL + mini-TrpRS, mini-TrpRS (20μl, twice daily, 600μg. Kg -1 .day -1 ). 3.2.Measurement of irreversible ischemic injury(TTC staining) No myocardial infarction was found in the sham group, , but obvious infarction areas were seen in other groups. Compared with CAL group (9.3%,15.0%,30.9%,39.3%),the myocardial infarction size of mini-TyrRS group at 3rd, 7th, 14th, and 28th day were respectively 8.0%,10.9%,22.4%,28.1%, as for mini-TrpRS group, were 10.7%,18.8%,37.5%,51.2%, but only in day 14th and 28th has significant difference, p
Page 2 and 3: The 3 rd VACPS Research Workshop Pr
Page 4 and 5: Organizing Committee Victorian Asso
Page 6 and 7: Preface On the occasion of the publ
Page 8 and 9: Paper as a low-cost base material f
Page 10 and 11: Acknowledgements We gratefully ackn
Page 13 and 14: Career planning and Job Interview i
Page 15 and 16: The role of mitochondria in atrial
Page 18 and 19: Track-Before-Detect Procedures for
Page 20 and 21: 3. Dynamic model for approaching ta
Page 22 and 23: ⎧π0 if j = i and Cx ij k > δij
Page 24 and 25: k k k k ( ) p( p Ζ | X , B = ∏ z
Page 26 and 27: As illustrated in Fig.4, the struct
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Page 30 and 31: (a) (b) Fig. 7. mesh plot of the re
Page 32 and 33: Definition 2 The probability of tar
Page 34 and 35: Acknowledgments Xiaobo Deng would l
Page 36 and 37: speed, several methods can be utili
Page 38 and 39: Where u ′ , v′ ) is the point p
Page 40 and 41: ase on SIFT are also unsuitable to
Page 42 and 43: Demonstration and Performance Analy
Page 45 and 46: Effect of mini-tyrosl-tRNA syntheta
Page 47: 2.1 Materials Sprague-Dauley(SD) ma
Page 51 and 52: mount/0.1mm 2 , as for mini-TrpRS g
Page 53 and 54: 3.4.The mRNA expression of mini-Tyr
Page 55 and 56: 9.Du XJ, Gao X, Ramsey D. Surgical
Page 57 and 58: Distribution of three forms alpha-m
Page 59 and 60: Materials and Methods Ethics All an
Page 61 and 62: Fig.1 (A) Mass Spectrometry results
Page 63 and 64: indicated that there is more des-α
Page 65 and 66: Fig.6 (A) Mass Spectrometry results
Page 67 and 68: transcription-polymerase chain reac
Page 69 and 70: Investigation of the mechanism of t
Page 71 and 72: Dual mode roll-up effect in a multi
Page 73: Paper as a low-cost base material f
Page 76 and 77: cloud computing offers a cost-effec
Page 78 and 79: workflows. By comparing the generat
Page 80 and 81: [10] E. Deelman, G. Singh, M. Livny
Page 82 and 83: Web Page Prediction Based on Condit
Page 84 and 85: A Multi-modal Gesture Recognition S
Page 86: Searching for Fair Joint Gains in A
Page 89 and 90: environments. However, challenges a
Page 91 and 92: conclude that the hydrophobic PHB c
Page 93 and 94: Figure 4. (a) Energy transfer model
Page 95 and 96: 20 Intensity (/10 6 ) / a.u. 15 10
Page 97 and 98: (22) (a) Cho, J.; Quinn, J. F.; Car
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Grain refinement of pure magnesium
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Fig. 3 The optical and SEM microstr
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Conclusions (1) The large grains in
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[22-25]. Sorting of normal and Babe
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Figure 1. Г×Re[fCM] vs. frequency
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Figure 3. The DEP spectrum of cylin
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A B C Figure 5. The gradient of the
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Figure 7. SEM image of damaged elec
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Figure 9. Particle separation progr
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The separation efficiency can be im
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[37] A. Motayed, M. Q. He, A. V. Da
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Fig. 1. The transverse and longitud
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References: [1] Xiong Liu, Mark C.
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new tissue[10]. Most of these human
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2.8 Viability and morphology study
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Fig 3 and 4 SEM images of electrosp
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studies have indicated that surface
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Fig 10 Tensile Stress curve of elec
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well as on a usual nanofibrous scaf
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Uniform Coating of WO x on TiO 2 Na
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Union as a Social Regulator of Mark
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associated with a reinvention of in
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enterprises…There obviously is no
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Union assistants participate in org
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[5] Deyo, Frederic C and Ağartan,
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Organizational Capabilities • Lea
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Table 1. Correlations, Reliabilitie
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[18]. Jap, S. D. (1999). Pie-expans
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Early Childhood Education Matters:
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decisions on selection of child car
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Noble, K. (2007). Parent choice of
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An Exploration of Country of Origin
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Supercontinuum generation for fiber
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Gas chromatographic retention indic
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Outward Foreign Direct Investment f
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Fiber Nonlinearity Precompensation
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Improved Nonlinearity precompensati
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