Workshop proceeding - final.pdf - Faculty of Information and ...
Workshop proceeding - final.pdf - Faculty of Information and ...
Workshop proceeding - final.pdf - Faculty of Information and ...
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Effect <strong>of</strong> mini-tyrosl-tRNA synthetase / mini-tryptophanyl-tRNA synthetase<br />
on ischemic angiogenesis in rats with acute myocardial infarction<br />
Rui Zeng 1 * , Yu-cheng Chen 1 , Zhi Zeng 1 , Wei-qiang Liu 1 , Xiao-xia Liu 2 , Rui Liu 3 , Ou<br />
Qiang 3 , Xian Li 3 , Smith AI 4<br />
* Presenter<br />
1. Department <strong>of</strong> Cardiology, West China Hospital, School <strong>of</strong> Clinic Medicine, Sichuan<br />
University, Chengdu ,China .610041<br />
2. Department <strong>of</strong> Epidemiology <strong>and</strong> Health Statistics,School <strong>of</strong> Public Health, Sichuan<br />
University,Chengdu ,China .610041<br />
3. Laboratory <strong>of</strong> Peptides Related with Human Diseases,The National Laboratory <strong>of</strong><br />
Biomedicine, Sichuan University, Chengdu,China .610041<br />
4. Department <strong>of</strong> Biochemistry <strong>and</strong> Molecular biology, Monash university, Melbourne,<br />
Australia.3800<br />
Background: To clarify the mechanism <strong>of</strong> the angiogenesis effect <strong>of</strong> mini-TyrRS/mini-<br />
TrpRS in rodent primates with acute myocardial infarction. Methods: Left coronary artery<br />
ligation was used to establish the model <strong>of</strong> acute myocardial infarction in rats (Sprague-<br />
Dauley male rats,250-300g, 2-3 months old), mini-TyrRS/mini-TrpRS were administered by<br />
coronary artery polyvinyl catheter injection to rats. Rats were r<strong>and</strong>omly divided into four<br />
experimental groups: sham operated group; coronary artery ligation (CAL); CAL+mini-<br />
TyrRS(20μl, twice daily, 600μg. Kg -1 .day -1 );. CAL+mini-TrpRS(20μl, twice daily, 600μg.<br />
Kg -1 .day -1 ). The experiment was carried out at 4 time points as the 3rd, 7th, 14th, <strong>and</strong> 28th<br />
day after ligation. To determine whether mini-TyrRS/mini-TrpRS affected the angiogenesis<br />
activity <strong>of</strong> rats with myocardial infarction, we measured the myocardial infarction size by<br />
TTC staining, <strong>and</strong> the density <strong>of</strong> capillary using immunohistochemistry staining to investigate<br />
the expression <strong>of</strong> VIII factor. Results: Compared with CAL group<br />
(9.3%,15.0%,30.9%,39.3%),the myocardial infarction size <strong>of</strong> mini-TyrRS group at 3rd, 7th,<br />
14th, <strong>and</strong> 28th day were respectively 8.0%,10.9%,22.4%,28.1%, as for mini-TrpRS group,<br />
were 10.7%,18.8%,37.5%,51.2%, but only in day 14th <strong>and</strong> 28th has significant difference,<br />
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