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6. Check that the pens work. Fill with ink if necessary, careful not to overfill as this can lead
to smudges on the traces.
7. Check that the mouthpiece is clean and that the sample tubing is free from water. If it is
not, flush the tubing with compressed air from the cylinder.
8. Do not switch on the pressure equipment as it is battery powered and this will just waste
the battery.
Calibration just before the study:
On chart recorder note:
o Date.
o Study name.
o Subject name.
o Parameters and voltage scales [these remained unchanged and would only have mattered
if there was a log scale change in the concentrations of the gases, pressure orflow that we
were measuring, see NO calibration belowl.
o Paper speed.
o Barometric pressure.
o Temperature.
o The background ambient NO level.
The procedures for calibrating the analysers:
Calibration of the NO analyser:
o Make sure the rotameter valve is closed.
o Tnro calibration: connect NO sampling probe to the black, cylindrical NO charcoal scrub
unit.
o NO concentration calibration one: connect NO sampling probe to the calibration gas 'one'
nozzle - turn on the cylinder and record the level noting on the chart record.
o NO concentration calibration two: connect NO sampling probe to the calibration gas 'two'
nozzle - turn on the cylinder and record the level noting on the chart record.
o Turn off the cylinders.
o Connect the sample port to the mouth-piece.
o Run the chart recorder to record both NO and COz baseline ambienUroom air values and
note results on the chart record.
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