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keys but it is currently in the middle of the range suggested by the manufacturers, and
used for all the early assessments).
o Tnro calibration: connect the COz sampling probe to the NO/i.{z tubing (NO calibration
gas) and press the CAL button to accept the zero calibration - mark on chart record - turn
off the gas.
o COz calibration gas one -
connect the COz sampling probe to the COz calibration gas
nozzle used and press CAL button to accept calibration - mark on the chart recorder - turn
off the gas.
o Barometric calibrations: Press the CAL button until the barometric pressure is displayed -
press the up arrow to set the barometric pressure - the COz bobbin will fall for 3 seconds
and then rise back up again and the current barometric pressure will be displayed - make a
note on chart record.
o Connect the sample port to the mouthpiece.
o Press display on the front panel to obtain the end-tidal COz display.
o Run the chart recorder to record both NO and COz baseline values noting the ambient
levels (more important for the NO recordings) (see Figure 6.1).
Calibration of the flow rotameter:
o Tnro calibration: with the air supply turned off and the rotameter needle valve turned off -
connect the air supply tubing to the bottom inlet of the rotameter - turn the air supply on -
run the chart recorder and label the trace.
o Calibration levels: open the valve until the rotameter bobbin moves and calibrate to
l50mls/min, 22lmlslmin, 325mls/min, 40Omls/min, 500mls/min, 600mls/min,
700mls/min, 800mls/min, 900mls/min, 1000mls/min at each level run the chart recorder
each time and note the calibration on the chart record - beyond this range the readings
become less stable when repeated over time and therefore less likely to be accurate.
o Leave the rotameter valve open - turn off the air supply, the bobbin will fall to zero
releasing the pressure in the air line (see Figure 6.2).
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