Acta 93.indd - Výzkumný ústav Silva Taroucy pro krajinu a okrasné ...

populations and their managed counterparts. A slightlyincreased frequency occurrence of haplotype A has as a rulebeen observed in managed populations. The populationson the localities Badín and Dobroč have also deviated fromthose in Stužica in haplotype A proportions detected in adulttrees and in their regenerated progenies. The populations ofthe former share a lower proportion of the haplotype A inregenerants than in adult trees, whereas the figure in Stužicais opposite. However, the variable as it seems, the chi-quadrattest has not confirmed statistical significance in the haplotypeA/B proportions between neither individual primeval standsanalyzed nor between the primeval populations and theirmanaged counterparts. The absence of these differences refersto both adult trees and regenerants.Based on allelic frequency distribution of the 14 enzyme geneloci, the basic parameters of genetic diversity were calculatedfor the primeval and managed populations of silver fir. Theproportion of polymorphic loci varied considerably amongthe populations ranging between 0.5 and 0.92 (Table 2). Threeloci (LAP-B, IDH, F EST) were found to be polymorphiccompletely as contrasted with a monomorphic nature of theShDH-A in all the populations surveyed. The remaining lociexhibited a variable degree of polymorphism contributingmore or less to differentiation of individual populations. Ofthese, the six loci (LAP-A, 6PGD-A, 6PGD-B, ShDH-B,MDH, PGI-B) are highly polymorphic in all the populationsanalyzed, whereas the four loci (GOT-A, GOT-B, PGI-A,GDH) are prevailingly monomorphic. The populations onthe localities Badín and Stužica share similar proportions ofpolymorphic loci (0.78–0.92 and 0.64–0.92) with number ofobserved alleles 1.92–2.42. Possessing reduced proportion ofpolymorphic loci (0.5–0.78) and lower number of observedalleles (1.57–2.00), the populations in Dobroč deviate fromthe populations mentioned above. The same tendency isalso characteristic for the effective number of alleles whichrefers to the measure of genetic diversity. The populations inBadín and Stužica were comparable in this respect possessing1.39–1.47 and 1.36–1.55 effective alleles, respectively, but thefigure in Dobroč was lowered little. The calculated numberof effective alleles of the latter ranged within the limit of1.27–1.38 only. Th data presented above refer to the regionaldifferences of silver fir populations involving both adult treesand regenerants of the primeval and managed stands of thespecies on the respective localities in Middle and EasternSlovakia.As far as the differences between primeval and managedpopulations are concerned, the figure is rather variable. Atthe level of adult individuals, the proportion of polymorphicloci was found to be the same in Badín (0.78) but reducedin Dobroč and Stužica primeval populations (0.57 vs 0.78in managed stand; 0.64 vs 0.71 in managed stand). Fromthe standpoint of relationship between adult trees andregenerants, an increased proportion of polymorphic lociprevailed in regenerants of the individual stands except forthe primeval and managed populations in Stužica where thereversed relationship was observed. Dobroč populations wereexceptional also with respect to the number of observed allelespossessing higher number of alleles in mature individuals thanin regenerants of both primeval and managed populations.An opposite figure was found to be valid for Badín andStužica stands with a higher number of observed alleles inregenerants (Table 2). To some degree analogous situationwas also characteristic for the number of effective alleles.The discrepancy concerned only the primeval populations inBadín and Stužica and/or managed stands Palota with highernumber of effective alleles in adult trees.The expected heterozygosity as principal components ofthe genetic diversity has reached a higher level in primevalTable 1. Chloroplast DNA haplotype proportions in three primeval populations of silver fir and intheir corresponding managed counterparts as revealed at the adult individuals level and in regenerants(Pomer haplotypov chloroplastovej DNA pri troch populáciach jedle bielej v pralesoch a u priľahlýchobhospodarovaných porastoch jedle zistený na úrovni dospelých jedincov a regenerantov)PopulationsNumber ofcpDNA haplotypesindvidualsABBadin – primeval stand, adult trees 28 16 (57.1 %) 12 (42.8 %)Badin – primeval stand, regenerants 67 35 (52.2 %) 32 (47.8 %)Badin – managed stand, adult trees 30 19 (63.3 %) 11 (36.7 %)Badin – managed stand, regenerants 76 32 (42.1 %) 44 (57.9 %)Dobroč – primeval stand, adult trees 33 19 (57.6 %) 14 (42.4 %)Dobroč – primeval stand, regenerants 55 37 (67.2 %) 18 (32.7 %)Dobroč – managed stand, adult trees 42 25 (59.5 %) 17 (40.5 %)Dobroč – managed stand, regenerants 64 33 (51.6 %) 31 (48.4 %)Stužica – primeval stand, adult trees 30 12 (40.0 %) 18 (60.0 %)Stužica – primeval stand, regenerants 58 35 (60.3 %) 23 (39.7 %)Stužica – managed stand, adult trees 33 18 (54.5 %) 15 (45.4 %)Stužica – managed stand, regenerants 96 54 (56.2 %) 42 (43.7 %)Palota – managed stand, adult trees 33 16 (48.5 %) 17 (51.5 %)Palota – managed stand, regenerants 75 39 (52.0 %) 36 (48.0 %)71