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Title: Alternative Sweeteners

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Trehalose 437<br />

Table 4 The pH of 4% Trehalose Solutions Prepared in 0.02<br />

mM Buffers and Incubated for 8 and 24 Hours at 100°C<br />

Incubation time (hr)<br />

0 8 24<br />

3.46 3.41 3.83<br />

3.71 3.64 4.02<br />

4.25 4.22 4.50<br />

5.14 5.10 5.25<br />

6.69 6.67 6.52<br />

7.46 7.44 7.13<br />

8.25 8.14 7.64<br />

8.88 8.91 8.42<br />

9.86 9.85 9.16<br />

Mean change SD 0.04 0.03 0.37 0.19<br />

each sampling time. At 90 minutes the absorbance of the maltose sample was<br />

15-fold greater than that of trehalose. The increase in absorbance is related to<br />

the production of colors associated with the caramelization process. Trehalose<br />

resisted the production of such color and may be an effective inhibitor of undesired<br />

caramelization.<br />

G. The Maillard Reaction<br />

The stability of 10% solutions of trehalose or maltose were tested in 0.05 M<br />

buffered solutions (pH 4.0, 5.0, 6.0, 6.5, and 7.0) containing 1% glycine (HBC,<br />

Table 5 The Absorbance of 10% Trehalose and 10% Maltose<br />

Buffered Solutions (0.033 M Sodium Phosphate in Water)<br />

Incubated at 120°C for up to 90 Minutes<br />

Absorbance at 480 nm<br />

Incubation time (min) Trehalose Maltose<br />

0 0.005 0.000<br />

30 0.013 0.051<br />

60 0.010 0.121<br />

90 0.012 0.184

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