PNNL-13501 - Pacific Northwest National Laboratory

Table 1. Transient mass changes associated with media
following H 2O 2 treatment
M/Z = 44, 59, 60, 63, 72, 87, 88, 89
Table 2. Transient mass changes associated with cells
following H 2O 2 treatment
M/Z = 24, 35, 37, 43, 44, 51, 56, 67, 75
Table 3. Persistent mass changes associated with cells
following H 2O 2 treatment
M/Z = 41, 52, 65, 88
Table 4. Mass changes in hyperoxic mice
M/Z = 28, 31, 32, 33, 47, 36, 37, 39, 41, 42, 48, 49, 50,
52, 53, 54, 55, 57, 58, 59, 60, 61, 62, 64, 68, 69, 70, 72,
76, 77, 78, 80, 81, 82, 83, 84, 86, 88, 89, 92, 95, 96, 98,
99, 100, 101, 102, 103, 104, 105, 106, 108, 111, 112,
1113, 115, 116, 118, 119, 120, 122, 125, 127, 128, 129,
131, 133, 134, 135, 136, 137, 138, 140, 142, 145, 146,
151, 152, 154, 155, 156, 157, 158, 159, 160, 161, 1662,
163, 164, 165, 166, 180, 183, 200, 203, 205, 208, 209
Summary and Conclusions
A number of mass targets were identified in our study.
Clearly, a more complex data set was identified in our
preliminary studies in a hyperoxic whole animal model,
relative to cultured cells treated with hydrogen peroxide.
Of particular interest, a candidate biomarker
(mass/change ratio; = 88) was observed both in vitro and
300 FY 2000 Laboratory Directed Research and Development Annual Report
in vivo in response to reactive oxygen species exposure,
under conditions that are likely comparable to the steadystate
response to reactive oxygen species. We have
examined the literature for mass targets increased in
pathologies associated with an oxidative stress to
determine whether there is precedent for this M/Z = 88
target. A volatile M/Z = 88.2 was identified in burn
patients with infection, a condition associated with the
generation of an oxidative stress (Labows et al. 1980).
The M/Z = 88.2 was identified as isopentanol. Therefore,
future studies will be directed at determining whether the
M/Z = 88 target identified in our studies is also
isopentanol. The correlation between prooxidant states
and an increased detection of M/Z = 88 raises the
possibility that this mass may be a biomarker of oxidative
stress that can be measured noninvasively in real time.
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