PNNL-13501 - Pacific Northwest National Laboratory

More documents

Recommendations

Info

Laser Step 2. Incubate with biological sample (e.g., cell or tissue extract). hv Step 3. Add matrix, which dissociates the antigen-antibody bond, and analyze by MALDI-TOF-MS. Proteolysis can be added to identify the intact protein and identify peptide regions that contain covalent modifications. 52 FY 2000 <strong>Laboratory</strong> Directed Research and Development Annual Report Step 1. Prepare mAb microarray containing up to 100 spots. + + + + + + + Figure 4. Microarray procedures for protein analysis + TOF-MS Covalently attached antibody Protein attached to antigen Antigen
Coupled NMR and Confocal Microscopy for In Situ, Real Time Cellular Measurement Capabilities Steven D. Colson, Paul D. Ellis, Robert A. Wind, Eric J. Ackerman, Don S. Daly, Gary R. Holtom, Paul D. Majors, Kevin R. Minard, John M. Price Study Control Number: PN99009/1337 The purpose of this project is to develop an instrument that integrates magnetic resonance microscopy (MRM) and confocal optical microscopy (OM) so both can be exploited simultaneously for noninvasive analysis of live, large single cells or three-dimensional cell systems. This instrument may be considered as the next generation instrument for biochemical and biomedical research on a cellular level. Project Description A multidisciplinary research team is carrying out a project that will generate a novel microscope specifically designed for cellular research. The instrument will integrate magnetic resonance microscopy and confocal optical microscopy so both can be exploited simultaneously for noninvasive analysis of live, large single cells or three-dimensional cell systems. The work performed included 1) constructing, calibrating, and testing the optical and magnetic resonance components of the microscope; 2) developing software to operate the confocal microscope; 3) developing statistical and image processing methods to improve the information obtained with magnetic resonance microscopy; and 4) benchmarking magnetic resonance microscopy-only and optical microscopy-only activities to establish the state of the art in each imaging modality. The first combined images have been obtained of individual Xenopus laevis oocytes. These high-resolution optical images have been used to increase the spatial resolution and the contrast near the cell boundaries in the magnetic resonance images. Introduction Both magnetic resonance microscopy and confocal optical microscopy each have their unique advantages and disadvantages when applied to studies of live cells (Blümler et al. 1998; Aiken et al. 1995; Pawley 1995). For example, while optical images can be acquired with a high spatial and temporal resolution, the application of optical microscopy is largely limited to transparent and stained samples. Conversely, while magnetic resonance microscopy is well suited for optically opaque samples and generally requires no exogenous contrast agents, the method is sensitivity limited. Despite these shortcomings, however, magnetic resonance microscopy is extremely useful as it can provide both biophysical and biochemical information that cannot be obtained with optical microscopy, and that is particularly useful for understanding health and disease at a cellular level. Therefore, correlating the optical microscopy and magnetic resonance microscopy data provides significant advantages over each of the methodologies individually. The specific aim of this project is to construct a microscope that will make it possible to study cells simultaneously with optical microscopy and magnetic resonance microscopy. Then the data obtained with one technique will be used to guide and optimize measurements with the other methodology. For example, the high-resolution optical images can be used to select volumes of interest on which to perform local magnetic resonance spectroscopy and to increase the resolution and contrast in the magnetic resonance images. On the other hand, magnetic resonance microscopy can provide complementary information in opaque or partially opaque cell systems, where optical microscopy is of only limited use. Moreover, the combined microscope will provide simultaneous optical/magnetic resonance information about cellular events, thus correlating the optical and magnetic resonance data. Therefore, the integrated optical microscopy/magnetic resonance microscope will significantly improve the capabilities of both methodologies for cellular research. Results and Accomplishments Magnetic Resonance Microscopy We tested the performance of the magnetic resonance part of the compound microscope, and found that the magnetic Biosciences and Biotechnology 53
Page 1 and 2:
Laboratory Directed Research and De
Page 3 and 4:
Laboratory Directed Research and De
Page 5 and 6:
Computational Science and Engineeri
Page 7 and 8:
Policy and Management Sciences Asse
Page 9 and 10:
Introduction Department of Energy O
Page 11 and 12:
5. After reviews by the Technical a
Page 13 and 14:
• The Technical Council peer revi
Page 15 and 16:
methods applicable to combustion, s
Page 17 and 18: Summary Each national laboratory mu
Page 19 and 20: Study Control Number: PN0004/1411 A
Page 21 and 22: Table 1. Positive and negative ions
Page 23 and 24: m/z Arbitrary Units Figure 1. Mass
Page 25 and 26: introduce low-volatility compounds
Page 27 and 28: arrangement, but the charge is reta
Page 29 and 30: two liquid chromatographic gradient
Page 31 and 32: Integrated Microfabricated Devices
Page 33 and 34: Matrix Assisted Laser Desorption/Io
Page 35 and 36: Molecular Beam Synthesis and Charac
Page 37 and 38: temperature distribution of the des
Page 39 and 40: expected to result in significant a
Page 41 and 42: Study Control Number: PN99069/1397
Page 45 and 46: Seuss DT and KA Prather. 1999. “M
Page 47 and 48: Analysis of Receptor-Modulated Ion
Page 49 and 50: laboratory (Lu and Xie 1997; Lu et
Page 51 and 52: Automated DNA Fingerprinting Microa
Page 53 and 54: Comparison of 4 Xanthomonas Isolate
Page 55 and 56: Approach Hematopoietic (bone marrow
Page 59 and 60: Anti-Human lgG Human lgG Protein G
Page 61 and 62: Characterization of Global Gene Reg
Page 63 and 64: PCR products for immobilization on
Page 65 and 66: identify novel proteins of importan
Page 67: Immunoprecipitaton of tyrosinephosp
Page 71 and 72: In the optical microscopy image, th
Page 73 and 74: Development and Applications of a M
Page 75 and 76: Figure 3. Hepa-1 cells undergoing a
Page 77 and 78: cellular processing (such as post-t
Page 79 and 80: 8. Initial demonstration of an off-
Page 81 and 82: expression systems for several year
Page 83 and 84: accumulation of protein products, i
Page 87 and 88: Fungal Conversion of Agricultural W
Page 89 and 90: Fungal Conversion of Waste Glucose/
Page 91 and 92: Summary and Conclusions We demonstr
Page 93 and 94: are shown in Figure 1(a) and 1(b),
Page 99 and 100: Anchorage-Independent Growth (% con
Page 101 and 102: selection of seedlings demonstratin
Page 103 and 104: NMR-Based Structural Genomics Micha
Page 105 and 106: Solution-State Structure and Fold-C
Page 107 and 108: Plant Root Exudates and Microbial G
Page 109 and 110: 98 99 100 6 7 10 3 1 12 11 15 16 17
Page 115 and 116: Summary and Conclusions • The gre
Page 119 and 120:
Jones-Oliveira JB, JS Oliveira, HE
Page 121 and 122:
• securely moves files to a targe
Page 123 and 124:
Study Control Number: PN98013/1259
Page 125 and 126:
Plenary invited lecture, “The Ele
Page 127 and 128:
Figure 1. X3DGEN tetrahedral mesh o
Page 129 and 130:
Figure 5a. OSO volume rendering of
Page 131 and 132:
Development of Models of Cell-Signa
Page 133 and 134:
SOS p 23 EGFR Professor Rodland at
Page 135 and 136:
Oliveira JS, JB Jones-Oliveira, and
Page 137 and 138:
Figure 2. Associating a dataset mar
Page 139:
to 1) incorporate three-dimensional
Page 142 and 143:
shown in Figure 1. Simulated result
Page 144 and 145:
HostBuilder: A Tool for the Combina
Page 146 and 147:
Invariant Discretization Methods fo
Page 148 and 149:
Graphics, Inc., workstation. The co
Page 150 and 151:
Page 152 and 153:
Mixed Hamiltonian Methods for Geoch
Page 154 and 155:
guyanaite, and grimaldiite (see Fig
Page 156 and 157:
in the Environmental Molecular Scie
Page 158 and 159:
Simulation and Modeling of Electroc
Page 160 and 161:
Summary and Conclusions We implemen
Page 162 and 163:
Page 164 and 165:
Since the implementation of the gen
Page 166 and 167:
asis of previous performance, perce
Page 168 and 169:
Bioinformatics for High-Throughput
Page 170 and 171:
Summary and Conclusions In previous
Page 172 and 173:
User Cat - Supervisor (client) Anal
Page 174 and 175:
The second goal was to research way
Page 176 and 177:
Figure 1. A visualization technique
Page 178 and 179:
and interactions. The flexibility o
Page 180 and 181:
Page 182 and 183:
Page 184 and 185:
Figure 4. A filtered version of Fig
Page 186 and 187:
Development of Modeling Tools for J
Page 188 and 189:
Figure 2. Schematic of experimental
Page 190 and 191:
Integrated Modeling and Simulation
Page 192 and 193:
(a) (b) (c) (d) Figure 1. Results o
Page 194 and 195:
Thurman DA. August 2000. Collaborat
Page 196 and 197:
MARC Input initial m esh and result
Page 198 and 199:
(Johnson 1999; Colligan 1999; Gould
Page 200 and 201:
Modeling of High-Velocity Forming f
Page 202 and 203:
Global divergence error 1 10 -14 0
Page 204 and 205:
that users were unlikely to appreci
Page 206 and 207:
Earth System Science
Page 208 and 209:
the lateral extent of the plume so
Page 210 and 211:
Biogeochemical Processes and Microb
Page 212 and 213:
Analyses of populations of viable a
Page 214 and 215:
The first task was to establish fiv
Page 216 and 217:
purged for 2 minutes. The gas sampl
Page 218 and 219:
developing code architecture to min
Page 220 and 221:
Berkowitz, CM. June 2000. “Atmosp
Page 222 and 223:
environmental monitoring, 2) portab
Page 224 and 225:
corresponded to a current density o
Page 226 and 227:
Enhancing Emissions Pre-Processing
Page 228 and 229:
Environmental Fate and Effects of D
Page 230 and 231:
nitrogen and unpredictable eutrophi
Page 232 and 233:
Figure 1. Ordinary kriging of 2 m d
Page 234 and 235:
Page 236 and 237:
precipitation of calcite occurred i
Page 238 and 239:
Page 240 and 241:
Interrelationships Between Processe
Page 242 and 243:
phenanthrene resistant fraction con
Page 244 and 245:
injection of an immiscible gas phas
Page 246 and 247:
still some key kinetic issues that
Page 248 and 249:
Page 250 and 251:
Application of a Crop Model The res
Page 252 and 253:
The Nature, Occurrence, and Origin
Page 254 and 255:
Particle number concentration, 1/cm
Page 256 and 257:
geometry optimized AlOOH and FeOOH
Page 258 and 259:
Page 260 and 261:
allowable parameters using thermody
Page 262 and 263:
TCE + 3H + + 3Fe 2+ Fe)=> acetylene
Page 264 and 265:
Use of Shear-Thinning Fluids for In
Page 266 and 267:
concentration of 0.5% AMX was the m
Page 268 and 269:
Roberts AL, LA Totten, WA Arnold, D
Page 270 and 271:
tetra-scale computing, envisioned a
Page 272 and 273:
Page 274 and 275:
Energy Technology and Management
Page 276 and 277:
Figure 2. Setup for the second expe
Page 278 and 279:
phase. The algorithms will be teste
Page 280 and 281:
Page 282 and 283:
[Pb-212]/[Pb-212] final 100 10 1 0.
Page 284 and 285:
Plasma Particle Dielectric Fiber El
Page 286 and 287:
(species, sub-species, and sex), sp
Page 288 and 289:
Figure 3. Relative risk of bone and
Page 290 and 291:
Development of a Preliminary Physio
Page 292 and 293:
Development of a Virtual Respirator
Page 294 and 295:
Figure 3. Use of the chest cavity a
Page 296 and 297:
Examination of the Use of Diazolumi
Page 298 and 299:
Indoor Air Pathways to Nuclear, Che
Page 300 and 301:
Source Building Release Observed re
Page 302 and 303:
To illustrate the potential impact
Page 304 and 305:
Non-Invasive Biological Monitoring
Page 306 and 307:
Page 308 and 309:
Materials Science and Technology
Page 310 and 311:
Figure 1. (a) - (c) Successive magn
Page 312 and 313:
Component Fabrication and Assembly
Page 314 and 315:
Development of a Low-Cost Photoelec
Page 316 and 317:
elaxations, indicating the adequacy
Page 318 and 319:
Promising chemical durability, as m
Page 320 and 321:
Page 322 and 323:
Study Control Number : PN98028/1274
Page 324 and 325:
High Power Density Solid Oxide Fuel
Page 326 and 327:
Hybrid Plasma Process for Vacuum De
Page 328 and 329:
Page 330 and 331:
Matson DW, PM Martin, WD Bennett, J
Page 332 and 333:
We showed the proof-of-principle ap
Page 334 and 335:
Bandgap Energy (eV) 2.9 2.85 2.8 2.
Page 336 and 337:
Page 338 and 339:
Ultrathin Electrolyte Test Results
Page 340 and 341:
Detailed Investigations on Hydrogen
Page 342 and 343:
identified low energy step structur
Page 344 and 345:
Development of Novel Photo-Catalyst
Page 346 and 347:
-2 -1 Energy (eV) 0 1 2 Cu2O SrTiO3
Page 348 and 349:
Here, we perform adsorption and des
Page 350 and 351:
exceed those in the all-metal devic
Page 352 and 353:
Page 354 and 355:
Intensity (a.u.) 0 100 200 300 400
Page 356 and 357:
integrated voltage (V) 0.10 0.08 0.
Page 358 and 359:
The first step was to implement thi
Page 360 and 361:
Spontaneous Formation of Cu2O Nano-
Page 362 and 363:
Actinide Chemistry at the Aqueous-M
Page 364 and 365:
Figure 5. Close-up atomic force mic
Page 366 and 367:
Page 368 and 369:
Page 370 and 371:
to 300°C and 400°C. Unfortunately
Page 372 and 373:
Page 374 and 375:
Summary and Conclusions Transmutati
Page 376 and 377:
Page 378 and 379:
Advanced Calibration/Registration T
Page 380 and 381:
Figure 1a. Violet filter Figure 1b.
Page 382 and 383:
Autonomous Ultrasonic Probe for Pro
Page 384 and 385:
containing nitrous oxide, an optica
Page 386 and 387:
Chemical Detection Using Cavity Enh
Page 388 and 389:
Figure 3 is a color rendered simula
Page 390 and 391:
appropriate study has been performe
Page 392 and 393:
Exploitation of Conventional Chemic
Page 394 and 395:
Figures 3 and 4 illustrate the impa
Page 396 and 397:
Page 398 and 399:
enefits of using a modified spread-
Page 400 and 401:
Page 402 and 403:
Single Channel Signal 0.0025 0.0020
Page 404 and 405:
Therefore, in order to extract the
Page 406 and 407:
A next-generation technology is nee
Page 408 and 409:
Page 410 and 411:
Wind Dir. Figure 2. Negative ion co
Page 412 and 413:
Page 414 and 415:
Volumetric Imaging of Materials by
Page 416 and 417:
amplitude and peak frequency change
Page 418 and 419:
Page 420 and 421:
Figure 3. 13 C NMR spectrum of corn
Page 422 and 423:
Approach Two flow loops, one that o
Page 424 and 425:
Page 426 and 427:
Modification of Ion Exchange Resins
Page 428 and 429:
Permanganate Treatment for the Deco
Page 430 and 431:
Page 432 and 433:
minimized by medium exchange. After
Page 434 and 435:
Figure 1. Thermogravimetric analysi
Page 436 and 437:
Validation and Scale-Up of Monosacc
Page 438 and 439:
Concentration (g/100g solution) Con
Page 440 and 441:
Analysis of High-Volume, Hyper-Dime
Page 442 and 443:
Page 444 and 445:
ecent figure of merit values. Shoul
Page 446 and 447:
Probabilistic Methods Development f
Page 448 and 449:
a) Traditional PCA-based process co
Page 450:
Acronyms and Abbreviations 2-D two-
show all

PNNL-13501 - Pacific Northwest National Laboratory

Create successful ePaper yourself

Delete template?

Save as template?