PNNL-13501 - Pacific Northwest National Laboratory

Comparison of 4 Xanthomonas Isolates on a 47 Probe Array (REP PCR
Products)
Sample 1 Sample 2
011 023 035 049
005 017 029 043
010 022 034 048
004 016 028 042
009 021 033 047
003 015 027 039
008 020 032 046
002 014 026 038
007 019 031 045
001 013 025 037
006 018 030 044
bio 012 024 036
011 023 035 049
005 017 029 043
010 022 034 048
004 016 028 042
009 021 033 047
003 015 027 039
008 020 032 046
002 014 026 038
007 019 031 045
001 013 025 037
006 018 030 044
bio 012 024 036
001
027
032
Probe hit in both reps
for sample 1
Probe hit in one of
the reps for sample 1
Probe hit in both reps
for sample 2
011 023 035 049
005 017 029 043
010 022 034 048
004 016 028 042
009 021 033 047
003 015 027 039
008 020 032 046
002 014 026 038
007 019 031 045
001 013 025 037
006 018 030 044
bio 012 024 036
Sample 3
Probe hit in one of the reps
024
for sample 2
Probe hit
025
Both reps sample 3
Probe hit both reps
009
Sample 4
Sample 4
011 023 035 049
005 017 029 043
010 022 034 048
004 016 028 042
009 021 033 047
003 015 027 039
008 020 032 046
002 014 026 038
007 019 031 045
001 013 025 037
006 018 030 044
bio 012 024 036
Figure 3. Microarray fingerprints of four Xanthamonas
isolates, color-coded to highlight similarities and differences
in the respective profiles. Positive hybridization was
determined by arbitrary threshold values within the
Phoretix software package. Hatched spots represent probes
that were only scored positive by Phoretix in one of two
replicate hybridizations.
Publications
Call DR., FJ Brockman, and DP Chandler. 2000.
“Genotyping Escherichia coli O157:H7 using multiplexed
PCR and low-density microarrays.” Int. J. Food
Microbiol. (in press).
Call DR, DP Chandler, and FJ Brockman. “Fabrication of
DNA microarrays using unmodified oligomer probes.”
BioTechniques (submitted).
Biosciences and Biotechnology 37