RA 00015.pdf - OAR@ICRISAT

Table 32. Range of pod damage to pigeonpeas caused by different pests in samples collected from various
states of India (1975-1977).
Lcpidopterous
borers
(H. armigera,
Species
Bruchids
(Calloso-
E. atomosa; Podfly bruchus Hymenoptera Actual pod
State Maruca (Melanagro- chinensis; (Taraostig- damage by
testulalis) myza obtusa) C. maculatus) modes sp.) insects
(%) (%)
Uttar Pradesh 2.6-12.3 9.9-36.0 0 - 0 . 2 0 - 0.2 15.5-48.7
Bihar 3.1-31.3 8.5-56.3 0 - 0.7 Below 1.0 15.5-70.6
Orissa 11.8-28.2 23.5-28.1 1.0- 4.4 0.2- 1.9 37.5-52.4
Andhra Pradesh 15.3-67.9 1.1-14.6 0 -22.2 0 -34.3 18.2-71.4
Maharashtra 10.9-83.5 1.8-44.3 0 - 1 . 4 15.5-84.6
Karnataka 3.1-81.1 2.4-19.7 0.9-43.8 0.5-54.1 27.3-88.3
Tamil Nadu 12.4-73.7 0.6-32.6 1.1-46.6 0.7- 4.7 26.1-81.5
plasm for total protein content, and conducting
studies on techniques for estimating protein and
some of the limiting amino acids. These studies
are essential for identifying rapid and economical
procedures that will be needed for implementing
a breeding program for higher quality.
Comparison of Methods of Protein
Estimation
The Kjeldahl method of estimating total nitrogen
is considered to be the standard method. The
M K J (micro-Kjeldahl) method gives very similar
results, and we have used it as a standard method
in our studies. We also use the T A A (Technicon
Auto Analyzer) and DBC (dye-binding capacity)
methods. We found a very high correlation
(r = +0.96) between M K J and T A A when testing
whole seeds. However, the correlation of
M K J and DBC determinations was lower
(r = + .87). This lower relationship was attributed
to interference of seed coat pigments in the
DBC method. Whole seed having the same
protein content as dhal samples gave a higher
reading on the U D Y instrument used in the DBC
method (Fig 33).
60
50
40
30
D h a l
Whole seed
20 21 22 23 24 25 26 27 28
M i c r o - K j e l d a h l protein percent ( N x 6 . 2 5 )
Figure 33. Relationship between DBC method
and MKJ procedure in determining
protein content of pigeonpea.
When dhal samples were analyzed, both T A A
( r = +.96) and DBC (r = +.94) were highly
correlated with M K J . It was concluded that for
rapid screening of dhal samples, either DBC or
T A A could be used, while for screening wholeseed
samples T A A should be used.
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