Abstract Book - 3rd International Symposium on Medicinal Plants ...

Key words: Algeria, antimicrobial activity, Asteraceae, essential oil, Senecio perralderianus.
3.51 Phytochemical Investigation of an Anti-Diabetic Herb: Juglans regia L.
Leaves Cultivated in Algeria
Tigrine-Kordjani Nacéra and Boukhari Fayçal
Laboratoire d’Analyse Organique Fonctionnelle, Faculté de Chimie, Université des Sciences et de
la Technologie Houari Boumediene, BP 32, El Alia, Bab Ezzouar, 16111 Alger, Algérie
<strong>Abstract</strong>: Jugalns regia L. is a tree that belongs to the family Juglandaceae, it is commonly
called walnut. Different parts of this tree, especially the leaves, are used in traditional medicine to
treat several diseases including diabetes. The literature is very rich in information about the heavy
fraction including phenolic compounds, several studies have been conducted on oil fruits showing
that it is rich in polyunsaturated fatty acids, unlike the volatile fraction of walnut leaves was a
subject of limited investigations. The objective of this study was to determine, for the first time, the
chemical composition of the volatile fraction of fresh leaves of walnut cultivated in Algeria. The
extraction of essential oil was performed by conventional hydrodistillation (HD) and by microwaveassisted
hydrodistillation (MAHD). Chemical Identification by GC-MS and quantification of
constituents by GC-FID had shown a variation in phytochemical composition of essential oils
obtained by both techniques. Indeed, we identified 38 compounds in the essential oil extracted by
MAHD for one hour (1h) with a yield of 0.05% (w / w) and 37 compounds obtained by HD for three
hours (3h) of extraction with a lower yield 0.03% (w / w). The major compounds were α-pinene, β-
pinene, d limonene, germecrene D, caryophyllene and farnesene.
Key words: Distillation, essential oil, Juglans regia, medicinal plant, monoterpenes, walnut,
sesquiterpenes.
3.52 Isolation of Volatile Oil with Antifungal Activity from Horseradish
(Armoracia rusticana)
Vasas Gabor 1 , Gonda S 1 ., Nguyen Minh N 1 ., Bertóti R 2 ., and Kiss A. 3
1 Department of Botany, Division of Pharmacognosy, University of Debrecen .2 Department of
Microbial biotechnology and cell biology, University of Debrecen .3 Department of Organic
Chemistry, University of Debrecen
<strong>Abstract</strong>: Armoracia rusticana, Cochlearia armoracia, Armoracia lapthifolia are scientific names
that refer to a perennial plant commonly known as horseradish. Armoracia rusticana is rich in
glucosinolates (GLS), secondary products which play an important role in the plant's defensive
system. GLS consist of β-thioglucoside N-hydroxysulfates with a side chain (R) and a sulfur-linked
β-D-glucopyranose moiety. Myrosinase reacts with GLS resulting in an unstable aglucone –
thiohydroximate-O-sulfonates, which then give rise to different products depending on reaction
conditions and participation of other factors. Under the effect of myrosinase the products of GLS
hydrolysis reaction are mainly isothiocyanates (ITCs). It is obviously shown that allyl
isothiocyanate (AITC) and 2-phenylethyl isothiocyanate (PEITC) are the most common
components found ITC in horseradish root. Isothiocyanates (ITCs) have many pharmacological
effects, they're recognized as anti-carcinogenic, gastro-protecting, anti-microbial, cell cycle
inhibiting, apoptosis triggering etc. activities. Volatile oil of horseradish was isolated and collected
by steam distillation from the root of A. rusticana. The major and minor components of the
essential oil were identified and quantified by GC-MS (Agilent 7890A GC, Agilent 5975 MS).
Antifungal activity was measured by microdilution method in Aspergillus nidulans, A. fumigatus,
Candida albicans, Saccharomyces cerevisiae The essential oil yield was 0.1% on raw weight
basis. Allyl isothiocyanate, n-butyl isothiocyanate, 3-butenyl isothiocyanate, 4-pentenyl
isothiocyanate, 5-hexenyl ITC, 5-methylsulphinylpentyl isothiocyanate were identified from the
essential oil. The allyl isothiocyanate (AITC) and 2-phenylethyl isothiocyanate (PEITC) were the
main component of the volatile oil. Strong antifungal activity was detected in all tested organisms.
The most sensitive strains were the A. nidulans and C. albicans.
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