Abstract Book - 3rd International Symposium on Medicinal Plants ...

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<strong>Abstract</strong>: Field experiments were conducted during two successive seasons of 2009/2010 and 2010/2011 at a private farm in South Ghor area, Al-Karak, Jordan, to study the effect of humic acid foliar spray with three concentrations 0,0,100, and 200 mg/liter and three levels of nitrogen fertilizer, i.e. 0, 45,and 90 kg/ha pure nitrogen on urea form on Thyme (Thymus vulgaris L.) growth and productivity. Results showed plant height, herbage yield and oil (%) were influenced by foliar spraying of humic acid and with nitrogen fertilizer application and their interaction effects. Foliar spraying of humic acid at 100 and 200 mg/liter and nitrogen application at both doses (45 and 90 kg/ha) resulted in more vigorous plants and significantly increased herbage yield, plant height and essential oil (%) compared to their respective control. More growth and yield were associated with higher humic acid and nitrogen fertilizer treatments. Foliar humic acid application at 100 and 200 mg/liter combined with nitrogen fertilizer application (45and 90 kg/ha) led to maximize the growth and essential oil (%), although non-significant in most cases. Key words: Thyme( Thymus vulgaris L.), growth, humic acid, nitrogen fertilization. 4.24 Effect of Magnetic Field on Cardoon (Cynara cardunculus l.) Seedlings SHARAF-ELDIN Mahmoud 1,2 , ALGORASHY Assari 1 , SABRY Refaat 3,4 , and ELSAYED Ibrahim 3 1 Sara bint Rached bin Ghonaim research chair for cultivating non-traditional medicinal and aromatic plants, College of Sciences and Humanitarian Studies, Salman bin Abdulaziz University (SAU), P.O. Box 83, 11942 Alkharj, Kingdom of Saudi Arabia (KSA). 2 Department of Medicinal and Aromatic Plants Research, National Research Centre (NRC). 12622-Cairo, Egypt. 3 Salman bin Abdulaziz University (SAU), College of Sciences and Humanitarian Studies, Physics Department. PB 83, 11942 Alkharj, Saudi Arabia. 4 <strong>International</strong> Centre for Advanced Studies in Physical Sciences, Faculty of Physics & Astronomy, Ruhr University Bochum, D-44780 Bochum, Germany. <strong>Abstract</strong>: Application of electricity, magnetism, monochrome light, and sound can stimulate the growth of plants to a great extent. This little-known technology, called Electro-culture, can accelerate growth rates, increase yields, and improve crop quality. Electro-culture can protect plants from diseases, insects and frost. These methods also can reduce the requirements for fertilizer or pesticides. Growers can produce greater and better crops in less time, with less effort, and at a lower cost. The energies are applied to the seeds, plants, soil or the water and nutrients. Cardoon plant (Cynara cardunculus L.) would be one of the best plants suits for the reclaimed desert lands in the Kingdom of Saudi Arabia (KSA). Last year in the market the price of the fresh artichoke head was SAR 5.53/0.37g and for processed artichoke receptacle was SAR 11.50/400g. Here we report for the first time the cultivation of cardoon in the KSA in particular at Alkharj Governorate. A laboratory experiment was carried out during the successive season of 2011/2012 at the premises of the Sara bint Rached bin Ghonaim Research Chair for Cultivating non- Traditional Medicinal and Aromatic plants, SAU, KSA, to study the effect of magnetic field (MF) on cardoon (Cynara cardunculus L.) seedlings. Seeds of Cynara cardunculus L. (Compositae) of German origin were supplied by Jelitto GmbH, Germany. Seventy five mT MF was used as follows: three different MF exposure time were studied as MF1: 15 min., MF2: 30 min. or MF3: 45 min, and MF0: not exposed to MF (control) were studied. We found that magnetic fields (MF) had significant effects on most of agronomic parameters studied. Days to germinate, germination percentage, seedling length (cm), root length (cm), seedling fresh and dry weight (g) influenced with the increase in MF exposure time. Among various MF exposures time (MF2) exhibited the best growth attributes. Additionally, root system was influenced by MF exposure. Key words: Cardoon, Cynara cardunculus, magnetic field. 107
4.25 Antioxidants In Vitro Androgenic Culture of Aesculus hippocastanum Štajner Dubravka 1 , Popović Boris 1 , Ćalić Dušica 2 and Štajner Marijana 3 1 Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovica 8, 21000 Novi Sad,. 2 Institute for Biological Research "Siniša Stankovi ı", University of Belgrade, Despot Stefan ,Blvd. 142, 11000 Belgrade. 3 Emergency Centre, Clinical Centre of Vojvodina, Hajduk Veljkova 1, 21000 Novi Sad, Serbia. <strong>Abstract</strong>: In vivo antioxidant scavenging activity of leaves and seed embryos, and in vitro androgenic embryos of Aesculus hippocastanum was study. Total antioxidant capacity of all the samples of A. hippocastanum was determined using FRAP, DPPH, and NO• radical scavenger capacity. Antioxidant enzyme activities of superoxide dismutase, catalase, guaiacol peroxidase and glutathione peroxidas, reduced glutathione quantity, flavonoids, soluble protein contents, quantities of malonyldialdehyde, and •OH radical presence in the investigated plant samples were determined. The leaves of A. hippocastanum had stronger antioxidant activity, (higher activities of SOD, higher quantities of GSH, TSH, TP, scavenging abilities of DPPH and NO•, higher FRAP values and lowest quantities of •OH and MDA) than in vitro obtained androgenic embryos. Androgenic embryos of A. hippocastanum had low amount of antioxidants due to the controlled environmental conditions we employed (T, photoperiod, humidity, nutritive factors, pathogen-free), and it can be concluded that tissue culture methods produce optimal condition for the growth of androgenic embryos. The optimization of in vitro conditions for mass production of androgenic embryos could improve cultivation techniques, conservation of these species, as well as protection from leaf miners. Key words: Antioxidant, Aesculus hippocastanum, androgenic embryo, tissue culture. 4.26 Biotechnological Approaches for Cultivation of Rh. rosea - Endangered Medicinal Plant Tasheva, Krasimira and Kosturkova, Georgina Plant Growth and Devepolment Regulation Department, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria. <strong>Abstract</strong>: At present more than 50 000 plant species are used in the two major fields: the contemporary phytotherapy and the modern allopathic medicine. World wide about 2/3 of those 50 000 medicinal plants are harvested from nature. The share of the cultivated plants used in the pharmaceutical industry is quite small, yet, being only 10 % in Europe. The number of natural populations is decreasing progressively leading to local extinction of many species or degradation of their habitats. Bulgaria is in the leading world positions in export of wild medicinal plants. Annual harvest varies between 15 000 t and 17 000 t. Half of them are collected in the mountains while 80% of them are exported. Bulgarian medicinal plants are famous for their high content of biologically active substances. Rhodiola rosea is a medicinal succulent plant with limited area of distribution. The species is protected by Law in Bulgaria and other countries (Romania, Russia, UK etc). Roots and rhizomes contain biological active substances. Extracts from roots and rhizomes are used in prevention and treatment of some social important diseases – neurology, cardio-vascular, oncology etc. The plants need 4-6 years to accumulate the high content of basic/main/ biologically active substances. The biotechnological tools are important to select, multiply and conserve the critical genotypes of medicinal plants. Some of the most important is the clonal propagation of identically plants in in vitro condition. Plant material from wild growing plants and seeds (selected for high level of salidroside and rosavin complex) were used for initial experiments in vitro. Efficient systems for mass plant propagation and callusogenesis were established. Acclimatization of rooted rodiola plantlets in the greenhouse and in natural conditions was successful up to 70 % and 68 %, respectively. Phytochemical analysis did not prove biologically active substances in the roots of one month old regenerants in test tubes. However, salidroside was detected in the rhizome and roots of one and two year old regenerants grown in the mountains and in the green house. Key words: Biotechnological, cultivation, medicinal plant, mountain, phytotherapy, rosea. 108
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The 3 rd International</str
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المملكت األردنيت ا
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Welcome Dear Participants, It is a
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SCIENTIFIC COMMITTEE - Mohammad San
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INVITED SPEAKERS Prof. Dr. Alain Me
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1.3 Antioxidant Activity of Thapsia
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1.7 High Inhibition Effect on Lipid
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products are being used as a source
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Key words: Anti-microbia, antioxida
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1.18 Preparation and Characterizati
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1.22 Eucalyptus globulus: Polypheno
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1.26 Antioxidant and Antiulcerogeni
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hydroxyl radical scavenging and red
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1.34 Anti-Inflammatory Effects of H
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1.38 Evaluation of the Antidiabetic
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general pain. It can be used as bev
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activity, respectively. Two yeasts,
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concentrate; n = 111 versus 10 %; n
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highest TPC was observed in HZ ecot
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1.56 Acute and Chronic Toxicity of
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Ergosterol Found in Mushrooms and Y
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potential antimicrobial activity ag
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(11.3%). The antioxidant activity o
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pulicaria incisa (L) DC has antibac
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1.75 The Effect of Aloe Vera Leaf G
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1.79 Antioxidant Activity of the Aq
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4 minutes in room temperature. Thir
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1.86 Hepatoprotective and Hypolipid
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ingredient were developed. Nano-nio
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167,3 ± 3,72 min), comparable to G
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disease induced by AlCl3 on the pyr
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The 3 rd International</str
Page 65 and 66: sclerosis (limb), sore throat, toot
Page 67 and 68: 2.9 Traditional Pharmacy - Attar's
Page 69 and 70: 2.12 Traditional Uses and Scientifi
Page 71 and 72: 2.16 Survey of Medicinal Plants Fro
Page 73 and 74: 2.20 Ethnobotanical Study of Medici
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Page 77 and 78: 3.5 Comparison between Polyphenol C
Page 79 and 80: according to ISO 3632.The knowledge
Page 81 and 82: as 89,007±1,914 % and 59,639±3,82
Page 83 and 84: egard to isoflavone concentration a
Page 85 and 86: evaluation were included 4 species
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Page 89 and 90: 1 Sciences Chimiques de Rennes, Cat
Page 91 and 92: guidelines came into operation in t
Page 93 and 94: 3.37 Characterization and Optimizat
Page 95 and 96: of this result, ethyl acetate extra
Page 97 and 98: 3.45 Chemical Composition of The Ex
Page 99 and 100: leaves, the contents of phenolics a
Page 101 and 102: Key words: Antifungal, Armoracia ru
Page 103 and 104: Abstract: A light
Page 107 and 108: 4.5 Formation of Hawthorn, Crataegu
Page 109 and 110: silydanin ,) while MS media supplem
Page 111 and 112: 4.13 Organic Cultivation and Use of
Page 113 and 114: 4.18 Effect of Some Chemical and Bi
Page 115: cultured in vitro. The WHITE and DC
Page 119 and 120: mortality increased from 39.66 % at
Page 121 and 122: 5.7 The use of seaweed extract of P
Page 123 and 124: 5.11 Antioxidant and Antifeedant Ac
Page 125 and 126: carveol and δ- selinene, but inhib
Page 127 and 128: inhibition 95%. In the during of bi
Page 129 and 130: (Sulzer). All experiments were cond
Page 133 and 134: capturing genetic diversity before
Page 137 and 138: 7.5 Heavy Metal Content in Hypericu
Page 139 and 140: 8.3 Development of Medicinal Plants
Page 141 and 142: 8.7 Industrial Products from Indige
Page 143 and 144: evaluation improvement (P
Page 145 and 146: products. The applied mulberry frui
Page 147 and 148: 9.10 The Importance of Gentiana lut
Page 151 and 152: 10.4 Comparative efficacy of variou
Page 153 and 154: 10.8 Effect of Rhus coriaria L. in
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Page 157 and 158: 10.15 Comparative Supplemented Vita
Page 159 and 160: A. DJEBARA (66) A. John De Britto (
Page 161 and 162: Eftimova Jarmila (58) EL Tahir A (1
Page 163 and 164: Mushtaq Ahmad (143) N. Gourine (35)
Page 165 and 166: Zinchenko, Iryna (52) ZRIRA Saadia
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