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Abstract Book - 3rd International Symposium on Medicinal Plants ...

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Key words: Anti-microbia, antioxidant, Citrus lim<strong>on</strong>, gallic acid, micro-organisms, polyphenols.<br />

1.14 Antioxidant Activities in Calycotome villosa L. (Fabacées) from Nati<strong>on</strong>al<br />

Park of Bou Kornine, Tunisia<br />

Ben Jemia Mariem 1 , Hamdaoui Ghaith 1 , Bruno Maurizio 2 , Kchouk Mohamed<br />

Elyes. 1<br />

1 Laboratoire des Plantes Extrêmophiles - Biotechnologic Center Borj-Cedria Technopark, B.P.<br />

901, 2050 Hammam-Lif, Tunisia. 2 Department STEMBIO, Sect. of Organic Chemistry, University<br />

of Palermo, Viale delle Scienze, Parco d’Orleans II - 90128 Palermo, Italy.<br />

<str<strong>on</strong>g>Abstract</str<strong>on</strong>g>: The present study was c<strong>on</strong>ducted to evaluate the antioxidant activities of the<br />

methanolic extracts of Calycotome villosa leaves. Plant material was collected from the Nati<strong>on</strong>al<br />

Park of Bou Kornine (Tunisia) and fresh plant samples were dried and ground into a fine powder<br />

and extracted with methanol at room temperature for 30 min. Extracts were kept for 24 h at +4°C,<br />

then filtered, evaporated under vacuum and stored in sealed vials under N 2 , at +4°C. Antioxidant<br />

activity was determined by four different methods namely DPPH, β-carotene/linoleic acid, reducing<br />

power and metal chelating activity assay. Additi<strong>on</strong>ally total phenolic, flav<strong>on</strong>oid and tannins<br />

c<strong>on</strong>tents of the methanolic extracts have been quantified. Results showed that, Calycotome villosa<br />

leaves exhibited high phenolic c<strong>on</strong>tents, especially total phenols (34, 9 ± 0,01mg GAE g−1 DW)<br />

and flav<strong>on</strong>oids (18.99 ±0, 14 mgEC g−1 DW). Extracts exhibit a higher antioxidant activity with β-<br />

carotene/linoleic acid bleaching test (IC 50 = 29.8±0, 13µg /ml). EC 50 values of reducing power<br />

activity were 20±1, 13µg/ml. Calycotome villosa may be c<strong>on</strong>sidered as an interesting source of<br />

antioxidants.<br />

Key words: Antioxidant activities, Calycotome villosa L., leaves, polyphenols.<br />

1.15 Antimicrobial Effect of Essential Oil of Artemisia herba alba Asso.<br />

BENREBIHA F., IFTENE, N., EZZIAT, H., CHAOUIA, C., and BOUCHENAK F.<br />

Université SAAD DAHLEB, BLIDA, Algérie.<br />

<str<strong>on</strong>g>Abstract</str<strong>on</strong>g>: The white wormwood recognized as forage for Excellence for protein intake and it‟s<br />

medicinal whether for animal or human capital is a significant. Used for its traditi<strong>on</strong>al medicinal use<br />

and studied in recent years in several countries to determine its chemical compounds in its<br />

essential oil of interest to the pharmaceutical, cosmetic and food. In our work we started by<br />

extracting the essential oil by steam distillati<strong>on</strong> of water. The compounds of the essential oil were<br />

identified by CG/MS. Microbial activity is tested <strong>on</strong> three bacterial strains (E. coli, K. pneum<strong>on</strong>ia<br />

and S. aureus). The results of the analysis CG/MS showed that the major compound is camphor.<br />

Tests of antimicrobial activity revealed that the essential oil of Artemisia white has an inhibitory<br />

effect <strong>on</strong> the strains tested.<br />

Key words: Antimicrobial activity, camphor, E. coli, essential oïl, K. pneum<strong>on</strong>ia, S. aureus.<br />

1.16 Study of the Antioxidant, Inflammatory, Analgesic Activities, Acute and<br />

Subacute Toxicity of the Extracts of Thapsia garganica<br />

BERRI Yasmine, BEDJOU Fatiha and Bougoffa Khalida<br />

Faculté des sciences de la nature et de la vie,Département de physico-chimique biologie<br />

,Université A. Mira Bejaia-Algerie.<br />

<str<strong>on</strong>g>Abstract</str<strong>on</strong>g>: Thapsia garganica bel<strong>on</strong>gs to the family Apiaceae, widespread in the Mediterranean.<br />

They have several various biological properties which are due to their richness in bioactive<br />

substances. In this study, our interest has been, the <strong>on</strong>e hand, to the phenolic compositi<strong>on</strong>,<br />

8

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