Abstract Book - 3rd International Symposium on Medicinal Plants ...

Mekkiou R 1 ., Boumaza O 2 ., Seghiri R 1 ., Ameddah S 3 ., A. Menad 3 , D. Sarri 1 , S.
Benayache 2 and F. Benayache 1 .
1 Laboratoire de Phytochimie et Analyses Physico-Chimiques et Biologiques, Département de
Chimie, Université Mentouri, Constantine, 2 Laboratoire de Valorisation des Ressources Naturelles
et Synthèse de Substances Bioactives, Département de Chimie, Université Mentouri, Constantine,
Route Aïn El Bey, 25000 Constantine. 3 Laboratoire de Biologie et Environnement, Université
Mentouri, Route de Ain El Bey, 25000 Constantine, Algérie.
<strong>Abstract</strong>: This study concerns the establishment of a chemical screening of Santolina
rosmarinifolia species which are wide-spread in Algeria, and known locally as Al-Djaada. In
Algeria, the leaves and the flowers of this species are used in folk medicine for their
gastrointestinal properties, stimulating, expectorant. Its essential oil is used as an antispasmodic,
antimicrobial and antifungal. Several investigations reported that this genus reveal the presence of
some chemical groups. Extracts obtained after a hydroalcoholic extraction were subjected to a
qualitative analysis TLC polyamide (Thin Layer Chromatography), CP and quantitative analysis by
using the test of Folin-Cioculteu. These analyses of extracts (ethyl acetate and n-butanol) showed
the presence of polyphenols The analysis on CP chromatography of the ethyl acetate extract
allowed us to isolate four products in the pure state and to determine the structure of two of them,
of besides an isolated product of the n-butanol extract.The structures of the compounds were
elucidated by UV, IR, EIMS and extensive application of one and two dimensional NMR
spectroscopy as well as by comparing their spectroscopic data with those reported in the literature.
The test of the antioxidant activity (test in the DPPH) revealed that the extracts of flowers and
leaves of Santolina rosmarinifolia are active in various degrees.
Key words: Antioxidant activity,.chemical screening, flavanoids, polyphenols,
rosmarinifolia.
Santolina
3.36 Standardization and Application Study on Paronychia Argentea
Mohammad, Z., Yousif, H., and Suleiman Olimat
Department of Pharmacetical Sciences, Faculty of Pharmacy, University of Jordan, Amman,
Jordan.
<strong>Abstract</strong>: Paronychia argentea is a perennial herb, widely distributed in Jordan. Several studies
showed that Paronychia argentea has hypoglycemic activity, it has been proved to be useful as
gastric analgesic, bladder, prostate, abdominal ailments treatment, and stomach ulcers treatment,
and it also showed significant alpha amylase and acetyl cholinesterase (AChE) enzyme inhibitory
activity. The plant extract of Paronychia argentea showed antioxidant activity .Other in vivo and
invitro studies on different extracts from Paronychia argentea revealed the immunomodulating
activity of the plant. The aim of our study was to standardize the drug preparation by quantifying
the main active component using HPLC, application of P. argentea in therapy. One kilogram of dry
plant (Paronychia argenteae ) socked in 20 liters of propylene glycol ( 5%). The extract was filtered
and used as a stock in the experiment (5% stock solution). Also Vanilic acid standard material was
dissolved in methanol and injected in the HPLC system. Mobile phase was prepared as follows:
Methanol: Water containing 1% Acetic acid (30:70), HPLC system prepared using the above
mobile phase at flow rate 1.0 ml/min and wavelength 260 nm. Injection volume was 20µL. Results
showed that vanillic acid standard material injected in the HPLC system, a peak of vanilic acid
appears at 7.6 min, then a sample from the plant extract was injected and two peaks appear at 6.8
min, 7.6 min respectively. To check vanillic acid peak, small quantity of vanillin acid was added to
the extract and then injected, an increase in the peak appears at 7.6 min (in the previous injection)
indicates that peak at 7.6 min refer to vanilic acid in the extract. In conclusion, a standardized
HPLC method of analysis for the final dosage formula was developed successfully based on
vanilic acid as a reference standard. The method validation is proceeding to ensure that the
method is a stability indicating method for the formula
Key words: Paronychia argentea, Standardization, Vanillic acid, Natural Products.
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