Abstract Book - 3rd International Symposium on Medicinal Plants ...

chemicals was highly toxic against the pre-imago thrips. The mortality percentage of the thrips (1-
2-day old) after 72h was more than 90% in the ethanolic extract M.azedarach and P. harmala
treatments. It was approximately 89% and 77% in the ethanolic extract of C. siliquastrum and C.
officinalis, respectively. Moreover, the mortality percentage of the pest (5-6-day old) after 72 h was
more than 73% in each of the plant extracts. The highest insecticidal activity against the abovementioned
pre-imago thrips was recorded in F. assafoetida treatment (80.70%). The results could
be concluded that these plant derived chemicals may be applicable as a safe insecticide in T.
tabaci management programs.
Key words: Bioassay, chemicals, insecticidal activities, Thrips tabaci.
5.18 Assay for Biocontrol of Verticillium Wilt of Olive Trees (Olea eureupea)
by Using Actinomyctes Strains
Harir Mohamed, Belahcen Miloud, and Fortas Zohra
Laboratory of biology of microorganisms and biotechnology; Faculty of sciences department of
biotechnology university of Es Senia Oran Algeria.
<strong>Abstract</strong>: The diseases phytopathogenes are responsible for the enormous economic losses, of
which 70% are caused by fungus. To limit the damage caused by these diseases, the researchers
employed various means of fight. Among these means, the chemical fight was the most used
method. However, the exaggerated use of the chemicals leads to harmful side effects as much for
the plant that for its environment. Thus the biological fight proved to be the means of the most
respectful fight for the environment. From the grounds rhizospheric of the orchards of olive-tree of
the Algerian west and arid sols of the Algerian south we isolated 188 stocks from actinomycetes
showing different morphological characteristics. The tests of antagonistic activity of the isolates of
actinomycetes by various methods enabled us to select 05 isolates, named A48, B14, ST1, T4 and
T6, presenting considerable antagonist activity with respect to V. dahliae. A traditional taxonomic
study was first of all undertaken on the basis of morphological characters. This study made it
possible to show that 04 isolate on the other hand produce chains characteristic of the
Streptomyces kind the characteristics of A48 isolate made it possible to classify it with the
Nocardia kind. The selected isolates were the subject of kinetics of growth and of production of
antibiotics in two test and culture media were then carried out on V. dahliae. The results of this test
showed an activity much more important of A48 isolate compared to the other isolates, with the
maximum ones of production of antibiotics to the 8 and the 15th day of incubation. The best
solvent of extraction is the ethyl acetate or N-butanol. The CCM of the rough extract of filtrate of
culture of A48 isolate on liquid ISP2 showed the presence of one 05 bands. We supposed that
these antibiotics can be attached to the group of aromatic glycosylés. The application in vivo as of
these isolate showed an important degree of protection of the tomato screw plants - with -
verticilliose, of which the percentages vary between (42.16% and 61.44%).
Key words: Actinomycetes, Rhizosphere, Tomato, V. dahliae, antagonism.
5.19 Biological Control of Fusarium Root Rot Disease on Strawberry Plant
Huda Hazim Al-Taae
Plant Protection Dept. College of Agric. & Forestry Mosul Univ.Iraq.
<strong>Abstract</strong>: The results of field survey which was conducted in greenhouses subsidiaries (the
Plant Protection Department, Department of Horticulture and Nineveh plant cultivation) in the
province of Nineveh- Iraq presence of many pathogens, especially fungal infect strawberry plants,
and most frequently with the pathogenic fungus Fusarium solani, followed by Fusarium
oxysporium in three houses plastic mentioned. The incidence varied in Fusarium root rot in field
survey of the three sites were the highest in the greenhouse of the plant cultivation by of Nineveh
65%. And that this study is the first study of the most important diseases of strawberry, In the test
of effect several pesticides in the fungal diameter growth of F.solani In vitro. noted that the
concentration of (100 mg active ingredient / l L )was sufficient to inhibit growth, except fungicide
Tobsin was not effective in inhibiting growth of pathogenic fungus, where the percentage of
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