Abstract Book - 3rd International Symposium on Medicinal Plants ...

1.75 The Effect of Aloe Vera Leaf Gel in Promoting Wound Healing and As
an Antibacterial Agent
Qaragholi, Zena M.F., and AlKayyat, Ali
College of Pharmacy/ Dep.of Pharmacognosy and Medicinal Plants, Cultural Affaira, University of
Baghdad, Iraq.
<strong>Abstract</strong>: This study explored the effect of crude Aloe Vera leaf gel in promoting wound healing
and to investigate its antibacterial effect against some pathogenic bacteria in comparison with
standard antimicrobial agents as Gentamycin and also other medicinal plants as Nigella Sativa oil.
Culture medias as BHIB (Re-isolation of the test bacteria), Nutrient Agar ( Sensitivity test), Nutrient
broth (MIC& MBC), Gelatin Agar (Biochemical method), MacConkey agar ( Diag . Pseudo.A),
Mannitol Salt agar (Diag of Staph. Aerreus) and Milk agar (Biochemical Method). Chemicals and
reagents as H2O240% (Catalase test), Tetramethyl-p-Phynelene Diamine Dihydrochloride1%
(Oxidase test), Crystal Violate, Iodine solution, acetone, Safranin (Gram Stain), Normal saline,
Bounes Sol. And Gentamycin. Standard dilutions of Aloe Vera leaf gel were made from 10-100%,
its antibacterial effect had been examined by seeded agar method against Staphylococcus aureus
and Pseudomonas aeruginosa Gentamycin and Nigella Sativa oil were used for comparison. To
explore the effect of Aloe Vera in vivo, twenty four local male rabbits were used and divided into 4
equal groups. Each animal was wounded in both sides of the back region. The first group was
control, designed to observe normal wound healing. The second group was treated with crude
Aloe Vera gel twice daily for 10 days. While the third group was wounded and infected with
Staphylococcus aureus without treatment to observe the natural body defense. The last group was
infected with the same bacteria and treated with crude Aloe Vera. Left side wounds in all groups
were left as self-control. Statistical analysis showed a significant difference (P