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Abstract Book - 3rd International Symposium on Medicinal Plants ...

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8.5 Effects of Fining <strong>on</strong> Antioxidative Activity of Merlot Wine Obtained with<br />

Increased C<strong>on</strong>tent of Stem and Seed<br />

Puškaš, Vladimir and Miljić, Uroš<br />

University of Novi Sad, Faculty of Technology, Blvd. cara Lazara 1, 21000 Novi Sad, Serbia<br />

<str<strong>on</strong>g>Abstract</str<strong>on</strong>g>: The structure and amount of phenolic compounds in the wine depend <strong>on</strong> the grape<br />

variety, agroecologic and vinificati<strong>on</strong> c<strong>on</strong>diti<strong>on</strong>s. The influence of pomace enrichment with grape<br />

stems and seeds during macerati<strong>on</strong>, as well as the effect of comm<strong>on</strong>ly used fining agents<br />

(albumin, bent<strong>on</strong>ite, gelatine and PVPP) <strong>on</strong> antioxidative activity of Merlot red wines were<br />

investigated. Besides c<strong>on</strong>trol wine, produced from pomace which c<strong>on</strong>tained original c<strong>on</strong>tent of<br />

seeds, without stems, other wine samples were obtained from grape pomace supplemented by<br />

50% of previously separated stem or by 120 g seed/kg. The antiradical activity of red wines<br />

towards DPPH • and hydroxyl ( • OH) radicals was determined by electr<strong>on</strong> spin res<strong>on</strong>ance (ESR)<br />

spectroscopy. Enriching of pomace with 120 g seeds/kg resulted in increase of antioxidative<br />

capacity of wine. In wine enriched with tannins and flavan-3-ols from the seeds, the DPPH •<br />

radicals were completely eliminated (AA DPPH •=100%), while stem additi<strong>on</strong> scavenged 88.21% of<br />

these radicals (AA DPPH • of c<strong>on</strong>trol wine was 83.73%). Regarding the scavenging capacity against<br />

hydroxyl radicals, supplementati<strong>on</strong> of pomace with seeds increased this parameter for 20%, while<br />

stem was not so efficient (5% increase). Applied fining agents, except gelatin, showed insignificant<br />

(p=0.05) influence <strong>on</strong> DPPH • scavenging activity of all wine samples. On the other hand,<br />

antiradical activity towards hydroxyl radicals (AA• OH ) was mostly significantly changed after each<br />

fining treatment.<br />

Acknowledgments: The authors appreciate financial support from the Ministry of Educati<strong>on</strong> and<br />

Science of the Republic of Serbia (Project TR-31002).<br />

Key words: Antioxidative activity, ESR spectroscopy, fining agents, merlot wine, seed, stem.<br />

8.6 <strong>Medicinal</strong> <strong>Plants</strong> in Bermet, Serbian Aromatic Wine<br />

Miljić, Uroš and Puškaš, Vladimir<br />

University of Novi Sad, Faculty of Technology, Blvd. cara Lazara 1, 21000 Novi Sad, Serbia.<br />

<str<strong>on</strong>g>Abstract</str<strong>on</strong>g>; Bermet is aromatic, dessert wine with especially unusual and original taste. Bermet is<br />

made exclusively from grapes from hillside vineyards of mountain Fruška Gora (Vojvodina, Serbia)<br />

and it is enriched with up to 24 to 26 aromatic, medicinal herbs. Technology of Bermet producti<strong>on</strong><br />

is passed down from generati<strong>on</strong> to generati<strong>on</strong> and its recipe was kept as a closely guarded secret.<br />

This rare wine is produced in limited quantities, <strong>on</strong>ly 15,000 to 20,000 bottles a year. This fact<br />

gives to the Bermet a special value and impressiveness. Nowadays, it is known that main<br />

medicinal plants included in Bermet producti<strong>on</strong> are: wormwood (Artemisia absinthium), yellow<br />

gentian (Gentiana lutea), anise (Pimpinella anisum), cinnam<strong>on</strong> (Cinnamomum zeylanicum),<br />

bitterbloom (Centaurium umbellatum), black mustard (Brassica nigra L), coriander (Coriandrum<br />

sativum), cloves (Syzygium aromaticum), carob (Cerat<strong>on</strong>ia siliqua) and liquorice (Glycyrrhiza<br />

glabra). Besides these plants, orange and lem<strong>on</strong> peel, vanilla bars, dried figs and grape, as well as<br />

some quantities of nutmeg (Myristica fragrans), are added to Bermet. Pharmacological compounds<br />

from these plants have a powerful t<strong>on</strong>ic, antioxidative, antiseptic, antidiarrheic and antipyretic<br />

effect. These plants are often used to remedy indigesti<strong>on</strong> and gastric pain in Serbian folk<br />

medicine. They can be also used as a carminative and in regulati<strong>on</strong> of the menstruati<strong>on</strong>. Bermet,<br />

as a valuable herbal medicinal beverage, dem<strong>on</strong>strates the complementary effect of phenolic<br />

compounds from red wine and numerous pharmacological compounds from medicinal and<br />

aromatic plants added during its producti<strong>on</strong>. The aim of this paper was to emphasize the individual<br />

effect of these plants, in order to evaluate their c<strong>on</strong>tributi<strong>on</strong> to the medicinal value of this aromatic<br />

wine.<br />

Acknowledgments: The authors appreciate financial support from the Ministry of Educati<strong>on</strong> and<br />

Science of the Republic of Serbia (Project TR-31002).<br />

Key words: Bermet, medicinal plants, pharmacological compounds, Serbia, wine.<br />

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