Abstract Book - 3rd International Symposium on Medicinal Plants ...

3.5 Comparison between Polyphenol Contents and Antioxidant Activities of
Different Parts of Capparis spinosa L.
ARRAR Lekhmici 1 , BENZIDANE Nadia 1 , KRACHE Imane 1 , , CHAREF
Noureddine 1 Seddik KHENNOUF 2 and BAGHIANI Abderrahmane 1
1 Laboratory of Applied Biochemistry, Faculty of Nature and Life Science, University Ferhat Abbas,
Setif, 2 Laboratory of Phytotherapy applied to Chronic diseases, Faculty of Nature and Life Science,
University Ferhat Abbas, Setif, Algeria.
<strong>Abstract</strong>: Capparis spinosa L., Capparidaceae is a shrub growing widely in Algeria. Aqueous
and methanolic extracts of five parts were studied for their contents in polyphenols and flavonoids.
These extracts were used to evaluate their antioxidant and anti lipid peroxidation effects. Results
showed that methanolic extracts contain more total polyphenols and flavonoids than aqueous
extracts. Leaves and flowers are rich in either polyphenols or flavonoids while roots are the poor
ones. In the same way, all extracts have anti lipid peroxidation and antioxidant effects with a
dominance of flowers and leaves especially in the methanolic extracts (82,78 ± 2,64 and 80,94
±1,57 respectively). Seeds have acceptable effects followed by bud than roots. These results
confirm the literature and give a comparative data on the different parts of Capparis spinosa
extracts that can be used in the disease with oxidative stress.
Key words: Antioxidant, aqueous extract, Capparis spinosa, lipid peroxidation, methanolic extract.
3.6 Effect of Polyploïdization and Elicitation on Hyoscyamine Content in
Hairy Roots of Datura.
Belabbassi Ouarda, Khelifi-Slaoui Majda, Zaoui Djamila and Khelifi Lakhdar
Laboratoire des Ressources Génétiques et Biotechnologie, Ecole National Supérieure
Agronomique, 16200 El-Harrach, Alger.
<strong>Abstract</strong>: The hyoscyamine, a secondary metabolite, widely used in medicine, can be produced
from roots of Datura sp. (Solanaceae). However, its content in the spontaneous roots remains low;
hence the use of hairy roots (in vitro) to improve it. The production of hyoscyamine through
biotechnology is of great interest and has many advantages. The hairy roots (HR) are
characterized by a good genetic stability and a rapid growth. Indeed, the HRs of Datura
stramonium L. are widely studied in the perspective of improving the yield of hyoscyamine. This
study is part of this same perspective; it aims to study the effect of polyploïdization of HRs induced
by colchicine combined or not with an elicitation with the acetylsalicylic (AAS) or salicylic (AS)
acids on the HR content in hyoscyamine. Colchicine was applied at different concentrations and
periods, on a line (L DS ) of D. stramonium HR induced by Agrobactrium rhizogenes (strain A4). The
selection of tetraploïd HR lines was made by the cytogenetic analysis using light microscopy and
the quantification of their DNA. The selected HR lines are elicited or not by AAS or AS. The effect
of polyploïdization and elicitation was measured on the biomass dry weight of HRs and their
hyoscyamine content. The untreated HR line (L DS : control) with colchicine shows a diploid level
with 2n = 2x = 24 chromosomes. However, the HR lines treated with colchicine show, in most
cases, an endoreduplication of their genetic material. The survival rate of endoreduplicated lines
varies between 30% and 93%, depending on concentration and exposure time to colchicine. Thus,
two HR lines (L DS1 and L DS2 ) were selected after confirming their tetraploïd nature. Moreover, the
two tetraploïd HR lines (L DS1 , L DS2 ) show an increase in their biomass and hyoscyamine content in
comparison to the diploid HR Line (L DS ). Further elicitation by AAS or AS at the 10 -4 M
concentration of the selected HR lines causes respectively a low decrease or increase in dry
weight. However, the same treatments show a significant increase in the yield of hyoscyamine in
elicited HR lines. Furthermore, the combination of polyploidïzation and elicitation gives an even
more significant improvement in hyoscyamine content.
Key words: Colchicine, Datura stramonium, elicitation, hairy roots, polyploïdization.
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