Abstract Book - 3rd International Symposium on Medicinal Plants ...

167,3 ± 3,72 min), comparable to GVit C (HT50= 163,4 ± 9,10 min) and largely higher than the
native control (HT50= 147,7 ± 0,40 min). All results confirmed that the extracts have a dose
dependent effect on the growth of overall antioxidant defenses. This results supports the use of
this plant against anti-inflammatory diseases in traditional medicine.
Key words: Antioxidant activity, Centaurea calcitrapa, DPPH, flavonoids, hemolysis.
1.94 Plant Components Exhibit Pharmacological Activities and Drug
Interactions by Acting on Lipid Membranes
Tsuchiya Hironori 1 and Mizogami Maki 2
1 Department of Dental Basic Education, Asahi University School ofDentistry, Mizuho, Gifu 501-
0296, Japan and 2 Department of Anesthesiology and Reanimatology, University of Fukui Faculty
of Medical Sciences, Eiheiji-cho, Fukui 910-1193, Japan.
<strong>Abstract</strong>: The medicinal benefits of numerous plants are attributable to
phytochemicalcomponentslike flavonoids, capsaicinoids, stilbenoids, allyl sulfides, etc., which are
best known for having antiproliferative and antioxidant properties. In light of anovel mode of action
on lipid membranes, we studiedsuch pharmacological activitiesof plant components and verified
their possible interactions with membrane-acting drugs. Fluorescence polarization measurements
with different probes revealed that 1-50 μMphytochemicals actedon biomimetic membranes
prepared with phospholipids and cholesterol of varying compositions. Theystructure-dependently
changedthe physicochemical property, fluidity, of membranes by preferentially affecting the deeper
regions of lipid bilayers. In the structure and membrane activity relationship, greater potencies to
change membrane fluidity were closely associated with the polyphenol structure, especially
flavonoids with hydroxyl groups at the 3-, 3‟-, 4‟-, 5-, 5‟-and/or 7-position. Quercetin and (-)-
epigallocatechingallate, meeting the structural requirements, effectively inhibited at 1-10 μMboth
the proliferation of tumor cells (inhibition (%) against cell proliferation after 24 and 48 h culture:
23.3 ± 5.5 and 74.3 ± 5.7 for quercetin and 31.3 ±6.2 and 75.5±0.6 for (-)-epigallocatechingallate)
and the peroxidation of membrane lipids(inhibition (%) against 10 and 50μMperoxynitrite-induced
lipid peroxidation: 100±0.1 and 74.6±0.1 for quercetin and 96.8±0.1 and 74.9±0.2 for (-)-
epigallocatechingallate). These antiproliferative and antioxidant phytochemicals also changed the
fluidity of cell membranes simultaneously with exhibiting pharmacological activities. The
membrane action is, at least in part, mechanistically responsible for the disease preventive and
therapeutic effects of medicinal plants containing such phytochemical components. Among the
tested membrane-active phytochemicals, 25-500 μMphloretinand capsaicin concentrationdependently
decreased or increased the membrane-fluidizing effects of lidocaine and bupivacaine
of clinically relevant concentrations (increase (%) of anesthetic membrane effects:205.5 ±1.5 for
lidocaine and 125.6 ±1.0 for bupivacaine). These results suggest the possibility that medicinal
plant components may antagonistically or synergistically interact with local anesthetics byacting on
lipid bilayers to modify the membrane environments for sodium and potassium channels
embedded in biomembranes.
Key words: Antioxidant, antiproliferative, drug interaction, lipid bilayer, membrane fluidity.
1.95 Isolation and Purification of Antifungal Compounds from Cyanobacteria
(Spirulina platensis)
Vinay kumar, A.K.Bhatnagar and J.N.Srivastava ⃰
Department of Botany, Faculty of Science, Dayalbagh Educational Institute, Dayalbagh, Agra-
282110
<strong>Abstract</strong>: Pathogenic fungi constitute an important public health problem as yet unresolved. In
most African countries, traditional phytomedicines are used to control the disease. Various algae
are known for their various biological activities. In the present investigation Spirulina platensis was
tested for antifungal activity for in vitro at different concentration against three clinical isolates of
pathogenic fungi i.e., (Candida albicans MTCC-227, Microsporum canis MTCC-3270, and M.
fulvum MTCC-7675). Therefore, the main objective of this work was to look for active substances
that could be used as antifungal agents. To achieve this target, two different extract (Methanol and
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