Abstract Book - 3rd International Symposium on Medicinal Plants ...

of this result, ethyl acetate extract was chromatographed.During this study, the chemical screening
of the plant Genista quadriflora (species endemic in Algeria and Morocco), and also the
quantitative analysis (TLC polyamide and chromatography on paper) of ethyl acetate extract of the
aerial part shows presence of several chemical groups. The test of the antioxidant activity (test in
the DPPH) shows that extracts of aerial parts of Genita quariflora are actives.The chromatographic
study on silica gel of ethyl acetate extract allowed us to isolate and identify the secondary
metabolites of flavonoid and isoflavonoid type. The DPPH radical scavenging ability of different
extracts of Genista quadriflora was calculated in a dose- dependent manner. Different extracts of
this plant proved to be an effective scavenger of DPPH radicals. The experimental results show
that all substances had antioxidant activity in vitro but their capabilities differed for the different
indicators
Acknowledgements: The authors wish to thank Dr. D. Sarri (Biology Department, University of M‟Sila, Algeria) for
identification of plant material, and MESRS (Ministry of scientific research, Algeria) for financial support.
Key words: Antioxidant activity, flavonoid, isoflavonoid, Genista Quadriflora.
3.41 Morphological, Physical Chemistry Analysis and Secondary
Metabolites Investigation of Cistus creticus
R. Sahraoui 1 , S. Djellali 2 , and A. Chaker 1
1 Département Biologie and Ecologie Végétale, Université Ferhat Abbas, 19000, Sétif.
2 Département des Sciences de la Matière, Centre Universitaire de Bordj Bouarréridj, 34000, Bordj
Bouarréridj, Algérie.
<strong>Abstract</strong>: The aim of this work is to study the secondary metabolites and the physicochemical
properties of Cistus Creticus in addition to morphological characteristics for better knowledge. The
literature indicates that this plant shows anti-inflammatory and antiviral properties. Locally it is
used as sedative drink. The plant was collected from mountains surrounding the city of Batna
(East of Algeria). Morphological characteristics of leaves and trichomes type were determined;
leaves were then separated from stems, cleaned of impurities and dried for a few days at room
temperature in shaded and ventilated place before being grounded to a powder. The powder was
then subjected to the following analyses: i) phytochemical analysis using extraction with solvents
of increasing polarity and qualitative determination of secondary metabolites and ii) chemical and
physicochemical analysis where the humidity, percentage of ash and metals content are
calculated. The solvent extraction showed a high yield of polar compounds (5.76%) compared to
apolar compounds (1.08%) while the chemical tests reveal the presence of alkaloids, heterosides,
triterpenes, cyanidin, flavonoids, and saponosides. In addition, it was found that the ash of the
plant contains low amounts of toxic metals such as Hg and Pb which makes the plant safe to use.
Key words: Cistus creticus, Phytochemistry, Trichomes, Secondary metabolites.
3.42 Preliminary Study of an Algerian Astéraceae: Nauplius Graveolens
RAMDANE F., and HADJ MAHAMMED M.
Laboratory of Biogéochemistry. Ouargla University. Algéria.
<strong>Abstract</strong>: The chemical composition of essential oils obtained by steam water distillation from the
dried aerial parts of Nauplius graveolens were analysed by gas chromatography (GC-FID) and
coupled to mass spectrometry (GC-MS). By water steam distillation the dried aerial parts of
Nauplius graveolens yielded 0,08-0,1% of essential oils having an intense and penetrating odor.
By using GC and GC-MS analysis thirty three compounds were determined in the reported
essential oil representing75,34% of the total content. The major chemical constituents of this oils
belong to terpenes were: σ cadinene 33,75% 2, 6, 6- triméthylacétate de bicyclo hept-2- en- 4-ol,
13.91%, liguloxide 9.47%, mertenyl acétate 6,50%, β eudesmol 1,29% and α eudesmol 1,00%.
The essential oils were found to show a broad spectrum of antimicrobial activity against the tested
bacterial strains.
Key words: Astéraceaes, biological activity, essential oils, GC-MS, Nauplius graveolens.
3.43 Analysis of Anti-lipo-peroxidation of Medicinal Plant Daphne gnidium L.
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