Abstract Book - 3rd International Symposium on Medicinal Plants ...

4.16 Effect of Foliar Application of PRO.SOL Nutrient Solution and Liquorice
Extract on Growth and Flowering of Geranium (Pelargonium zonale L.)
Nasser, Z. S and Abbass, J. A
Facility of agriculture – Kufa University – Iraq.
<strong>Abstract</strong>: The world now avoidance from the use of chemical matters because the negative
effects of these chemicals on the environmental, plant and human and resort to use the crude
nature matters and plant extracts alternate from it . The Liquorices root extract of Glycyrrhia
glabra L plant is one of it, which use in the medicine more than 4000 years in the China as a drug
for cold and cough , also many studies and researches indicate that spraying Liquorices root
extract on plants improving growth parameters because these root extracts contain many
nutrients ( N, P, K, Ca, Fe, Zn and Mg ), Carbohydrates and Amino acids (Asparagine and Chlino (
as well as the active matter of Liquorice root Extract be characterized similar action like GA
hormone . because of that an experiment was conducted In the lath house of agriculture college
/university of Kufa , to study the effect of foliar application of PRO.SOL nutrient solution and
Liquorices root extract on growth parameters of Geranium (Pelargonium zonale L.) cultivar Hans
Rigler, included test of three concentration of nutrient solution PRO.SOL i.e. (0,1,1.5)gm.L -1 and
three concentration of Liquorices root extract i.e. (0,1.5,3.0)gm.L -1 , experiment was conducted as
factorial experiment in Randomized Complete Block Design (R.C.B.D) with two factors. Duncan's
Multiple Range test was used at probability of 0.05 to compared means. Results showed that
spraying plant with Nutrient Solution of PRO.SOL at concentration of (1.5)gm.L -1 or Liquorice root
extract concentration of (3.0) gm.L -1 significantly increased growth parameters ( plant height ,
numbers of total leaves , shoot dry weight , total roots number ,roots dry weight, leaf content of
total chlorophyll , potassium and iron) in spring season, and number flowers.floret -1 and first flower
diameter of the floret and flower content of anthocyanine in autumn and spring season compared
to the lowest values which produced from non-spraying plant (control). Results revealed that the
interaction between Nutrient Solution of PRO.SOL at concentration of (1.5)gm.L -1 and Liquorice
extract concentration of (3.0)gm.L -1 significantly increased plant height, number of total leaves,
shoot dry weight total roots number, roots dry weight and leaf contents of total chlorophyll,
potassium and iron in autumn season, and increasing significantly in the number of flowers.floret -1 ,
flower diameter of the first floret and flower content of anthocyanine in the autumn and spring
season compared to the lowest values which produced from control treatment.
Key words: Foliar, flowering, geranium, growth, liquorice extract.
4.17 In Vitro Multiplication of Achillea fragrantissima Forssk Sch. Bip
Laila SasiYounes 1 , Rida A. Shibli 1 , Tamara Said Al-Qudah 2
1 Department of Horticulture and Agronomy, Faculty of Agriculture, University of Jordan. 2 Hamdi
Mango Center for Scientific Research (HMCSR), University of Jordan, Amman, Jordan.
<strong>Abstract</strong>: Achillea fragrantissima L. is a wild medicinal herbal plant found in Jordan and
neighboring countries. The biodiversity of this plant is heavily subjected to loss because of heavy
grazing, land cultivation and collection by people to be used in folk medicine. In the current study,
increasing the mass production of this medicinal plant was achieved by in vitro propagation
techniques, rooting and acclimatization. Micropropagation of Achillea fragrantissimawas initiated
from seeds. Seeds were surface-sterilized and cultured on the surface of half-strength, fullstrength
MS (Murashige and Skoog) medium, or water-agar medium, with or without plant growth
regulators (PGRs). Complete germination of seeds (100%) was obtained in water-agar medium
and developed into plantlets with greater hypocotyl and root length, and full cotyledonary leaves.
Multiplication of mother stock was established on MS (Murashige and Skoog) media
supplemented with 1mg/L GA 3 after 4 weeks of culture. Proliferation was experimented with
different levels (0.0, 0.4, 0.6, 0.8, 1.2 or 1.6 mg/L) of BA, kinetin or 2ip. Maximum proliferation of
Achillea fragrantissima (8 shoots/ explant) was obtained when MS medium supplemented with 1.2
mg/L of kinetin was used. Rooting was experimented at different levels (0.0, 0.4, 0.6, 0.8, 1.2, or
1.6 mg/L) of NAA, IAA or IBA. Highest root number (9.80cm) and length (1.80 cm) was obtained at
0.4 mg/L NAA, while IAA failed to promote root induction. Rooted plantlets were successfully
acclimatized with 100% survival.
Key words: Achillea fragrantissima, forssk, in vitro, multiplication.
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