Abstract Book - 3rd International Symposium on Medicinal Plants ...

according to ISO 3632.The knowledge of phytochemical properties of saffron can also be used
during manufacturing of pharma-grade saffron extracts to steer the process towards a further
increase in marker compounds. These improvements of saffron extract quality and standardisation
allow a reproducibility of phytochemical properties, which is important for the reliability and
reproducibility of clinical effects.
Key words: Modern applications, traditional use, saffron.
3.9 Neolignans from the Marine Phanerogams Posidonia oceanica
Bitam Fatma 1 , Ciavatta, Maria Letizia 2 Manzo, Emilianno 2 ; Villani, Guido 2 and
Gavagnin, Margherita 2 .
1 Faculté des sciences, département de Chimie, Université de Batna, Batna 05000, Algeria. 2 Istituto
di Chimica Biomolecolare, C.N.R., Via Campi Flegrei 34, I-80078 Pozzuoli, Naples, Italy.
<strong>Abstract</strong>: Sea-grasses are the marine flowering plants that successfully grow in tidal and
subtidal marine environment. They are an ecologically important role for structuring a number of
ecosystems, stabilizing coastlines, providing food and shelter for diverse marine organisms and
act as a nursery ground for many fishes of commercial importance. Posidonia oceanica is one of
these groups of plants which played this role in the Mediterranean basin. A number of
phytochemical investigations with different focus were carried out. The main compounds included
sterols and phenolic compounds such as: phenylmethane derivatives, phenylethane derivatives,
phenylpropane derivatives and their esters, chalkones, flavonols, 5α-cholestanes, and cholest-5-
enes. Some limited studied proved the presence of lignins in some seagrasses such as Posidonia
oceanica and zostera marina, but no mention on their lignan composition. Here, we report the first
chemical investigation of lignans of Posidonia oceanica. The roots were extracted with acetone;
the obtained extract was partitioned with chloroform and n- butanol giving two fractions. The
chloroform fraction was purified using different chromatographic methods, in particular the HPLC
technique. The spectroscopic experiences mainly the 1 H and 13 C NMR techniques, the IR and
mass spectrometry allowed us to identify the structures of three new neolignans (A, B and C)
containing a dihydrobenzo[b]furan skeleton. However, the relative stereochemistry of the C7-C8
ring was established to be trans by the NOE difference experiment while the absolute
stereochemistry is under study.
Key words: Chloroform fraction, lignin, Neolignans, Posidonia oceanica, sea grasses.
3.10 Analysis of Volatile Products in Honey by SPME –GC-MS
Boudina Ali, Benmssedak Malak, and Yassaa ,Noureddine
USTHB, University of Sciences and Technology Houari Boumediene, Faculty of Chemistry, B.P.
32 El-Alia, Bab-Ezzouar, 16111 Algiers, Algeria.
<strong>Abstract</strong>: Pesticides play a beneficial role in agriculture, because they help to combat the
variety of pest that destroy crops, even though small amounts of pesticide residues remain in the
food supply, constituting a potential risk for the human health, because of their sub-acute and
chronic toxicity. Honey samples collected from different Algerian locations have been analysed by
means of Solid Phase MicroExtraction (SPME) and gas chromatography/mass spectrometry in
order to investigate the eventual presence of pesticide residues. The use of solid SPME fibre
namely 75 µm Carbowas/DVB allowed a number of honey-related compounds to be identified
along with several contaminated products. The application of this technique in the analysis of
pesticide residues in honey will be discussed with regards to the simplicity, the solventless and
rapidity of the SPME technique.
Key words: Gas chromatography, honey, micro extraction, pesticide residues, solid-phase.
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