Abstract Book - 3rd International Symposium on Medicinal Plants ...

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weight of leaves and small corms diameter were in their best position through spraying 5% of suspension. The least growth was dedicated to the non-treatment in each characteristic. Furthermore, the highest numbers of small corms in each main corm was harvested through out interaction of forth harvesting, 5 and 7% of suspension spraying and complete fertilizer. However based on economical and environmental conditions, using treatment of 5% is offered. In whole levels of suspension spraying the best date of harvesting was forth harvesting (7 Sep.) considering the main corm‟s diameter. Interaction between forth harvesting with non-suspension spraying was offered for increasing main corm‟s dry weight. Considering whole measured traits we suggest that Saffron‟s farmer use interaction between the forth harvesting treatment (7 Sep.) and 7% suspension spray to achieve the satisfied results. Key words: Corm harvesting, main corm, saffron, suspension sprayin. 4.20 Effects of Biological (Bio-Stimulators) and Chemical Fertilizers Foliar Application on fenugreek (Trigonella foenum-gracum L.) Under Drought Stress. Masomeh Mohammdi 1 , Heshmat Omidi 2 , Ali Mehrafarin 3 , and Hasan Ali Naghdi Badi 3 1 MSc Student, Department of agronomy and plant breeding, Agricultural College, University of Shahed. 2 Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahed university. 3 Department of Cultivation and Development, Institute of Medicinal Plants, ACECR, Karaj, Iran. <strong>Abstract</strong>: To investigate the effects of drought stress and chemical fertilizer and bio-stimulators on yield and some quantitative characteristics of Trigonella foenum-gracum, a factorial experiment was conducted on the basis of completely randomized blocks design with three replicates in Department of Cultivation and Development, Institute of Medicinal Plants, ACECR in Karaj, Iran in 2011. Treatment included drought stress with levels of non stress (40%FC), average stress (55%FC) and Severe stress (70%FC) and fertilizer with levels control (A), aminolforte (B), Fosnutren (C), kadostim (D), humiforte(E), humiforte+50% (NPK)(F), humiforte+100% (NPK)(G). Results showed that effect of fertilizer ×drought stress was significant (P≤0.01) on parameters of chlorophyl, plant height , petiole length, Root fresh weight, Root dry weight, total seeds fresh weight, total seeds dry weight, seeds thousand weight, the number of seeds per pod. So that was the most chlorophyll, petiole length, number of seeds per pod related to the combination treatment Fosnutren with non stress and most plant height, root dry weight, root fresh weight, seed dry weight related to the combination treatment humiforte+100% (NPK) with severe stress and most seeds fresh weight related to the combination treatment humiforte+100% (NPK) with average stress and most seeds thousand weight related to the combination treatment kadostim with severe stress. Key words: Biostimulators; chemical fertilizer, drought stress, fenugreek. 4.21 First Results on Germination of Embryos of the Yew Tree (Taxus baccata ssp communis) Collected in Chréa (Algeria) Morsli A., Ait Abdelkader N., Benassoula F., Benslimani N. and Khelifi L. Laboratoire de Recherche sur les Ressources génétiques et Biotechnologies (LRGB), Ecole Nationale Supérieure Agronomique (ENSA), El Harrach, Alger, Algeria. <strong>Abstract</strong>: The yew tree (Taxus baccata sp communis) is a leading species in medicinal plant. It is producing secondary metabolites of pharmaceutical interest, such as taxol (antimitotic molecule). However, this species does not regenerate itself in Algeria through seeds which are characterised with embryo dormancy. This earned it to the status of endangered species. The valorisation of its metabolite must, therefore, pass through its regeneration and its conservation and multiplication both in situ and ex situ. To do this, it is imperative to lift this embryo dormancy. Indeed, sterile embryos are isolated from freshly harvested seeds and then disinfected and 105
cultured in vitro. The WHITE and DCR culture media, stages of seed maturity and growing conditions (darkness / light) were tested. The best germination rate (83.33%) is obtained from embryos isolated from bright green seeds, grown on the WHITE culture medium in the dark. The addition of activated charcoal and/or PVP improves significantly the embryo germination quality on the one hand, and prevents culture browning, on the other hand. The seedlings thus obtained constitute a good starting material for taxol producing via biotechnological process or in the open field after the reintroduction of the acclimated seedlings in their natural environment. Key words: Embryo germination, in vitro, regeneration, Taxus baccata. 4.22 Flavonoid Accumulation in Hypericum androsaemum Cell Cultures: Involvement of cAMP Paranhos, A. Faculdade de Farmácia and Centro de Estudos Farmacêuticos, Universidade de Coimbra, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal. <strong>Abstract</strong>: Hypericum androsaemum L. is an herbaceous plant found in damp or shady places throughout Europe and has been used in traditional medicine owing to the diuretic and hepatoprotective properties of its aerial parts. These biological effects are attributed to the diverse flavonoids and phenolic acids known to be present in this species. Cell cultures have been recognized to represent a potential alternative source of secondary plant constituents and also a valuable tool for the study of biosynthetic pathways and regulation of metabolite synthesis. Cell suspension cultures established from hypocotyl-derived callus of H. androsaemum were reported to accumulate low amounts of flavonoids, with the highest levels being observed during the stationary phase (day 14). More recently, it was shown that treatment of 11-day-old cultures for 72h with 15 mM CaCl 2 or 5 µM calcium ionophore A23187 increased considerably the accumulation of flavonoids and the activity of phenylalanine ammonia-lyase (PAL), a key regulatory enzyme of phenylpropanoid metabolism assayed. Since calcium and cAMP systems often interact to regulate cell functions, similar experiments were carried out using either 100 µM dibutyryl-cAMP, a membrane-permeable cAMP analogue, or 100 µM IBMX, a cNMP phosphodiesterase inhibitor. Treatments with these modulators of intracellular cAMP also induced a marked increase in both PAL activity and flavonoid content of cells recorded on day 14. On the other hand, the addition of 100 µM 8-Br-cGMP (a membrane-permeable, phophodiesteraseresistant cGMP analog) had no significant effect on flavonoid production, even though the PAL activity levels were higher than in the untreated cells. In contrast, treatment of cultures with the adenylyl cyclase activator forskolin (20µM) enhanced the accumulation of flavonoids without any significant increase in the levels of PAL activity. Taken together, these findings point to a possible involvement of cAMP signaling in the biosynthesis of flavonoids by H. androsaemum cell cultures and also indicate that PAL activity is not always correlated with the accumulation of these compounds. Key words: Cell culture, flavonoid, forskolin, Hypericum androsaemum, metabolism. 4.23 Growth and Yield of Thyme (Thymus vulgaris L) as Influenced by Humic Acid Foliar Spraying and Nitrogen Fertilization Saddam Aref Al-Dalain 1 ., Jawad A. Al-Dala'een 2 ., Adel H. Abdel-Ghani 3 ., Haditha A.Thalaen 4 ., and Khalil A. Al-Dhala'in 5 1 Al-Shoubak University College, Al-Balqa’ Applied University ,Al-Salt 19117, Jordan. 2 Karak University College, Balqa’Applied University, P. O. Box 36 Karak (61151) Jordan. 3 Department of Plant Production, Faculty of Agriculture, Mutah University, P.O. Box 7, Karak, Jordan. 4 Plant Production -Vegetable Farming Since, P.O. Box 4,Karak – Jordan. 5 Southern Ghour Province Agricultural Directorate- Ministry Of Agriculture, Jordan. 106
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The 3 rd International</str
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المملكت األردنيت ا
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Welcome Dear Participants, It is a
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SCIENTIFIC COMMITTEE - Mohammad San
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INVITED SPEAKERS Prof. Dr. Alain Me
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1.3 Antioxidant Activity of Thapsia
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1.7 High Inhibition Effect on Lipid
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products are being used as a source
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Key words: Anti-microbia, antioxida
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1.18 Preparation and Characterizati
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1.22 Eucalyptus globulus: Polypheno
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1.26 Antioxidant and Antiulcerogeni
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hydroxyl radical scavenging and red
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1.34 Anti-Inflammatory Effects of H
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1.38 Evaluation of the Antidiabetic
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general pain. It can be used as bev
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activity, respectively. Two yeasts,
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concentrate; n = 111 versus 10 %; n
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highest TPC was observed in HZ ecot
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1.56 Acute and Chronic Toxicity of
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Ergosterol Found in Mushrooms and Y
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potential antimicrobial activity ag
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(11.3%). The antioxidant activity o
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pulicaria incisa (L) DC has antibac
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1.75 The Effect of Aloe Vera Leaf G
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1.79 Antioxidant Activity of the Aq
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4 minutes in room temperature. Thir
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1.86 Hepatoprotective and Hypolipid
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ingredient were developed. Nano-nio
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167,3 ± 3,72 min), comparable to G
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disease induced by AlCl3 on the pyr
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Page 65 and 66: sclerosis (limb), sore throat, toot
Page 67 and 68: 2.9 Traditional Pharmacy - Attar's
Page 69 and 70: 2.12 Traditional Uses and Scientifi
Page 71 and 72: 2.16 Survey of Medicinal Plants Fro
Page 73 and 74: 2.20 Ethnobotanical Study of Medici
Page 77 and 78: 3.5 Comparison between Polyphenol C
Page 79 and 80: according to ISO 3632.The knowledge
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Page 83 and 84: egard to isoflavone concentration a
Page 85 and 86: evaluation were included 4 species
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Page 89 and 90: 1 Sciences Chimiques de Rennes, Cat
Page 91 and 92: guidelines came into operation in t
Page 93 and 94: 3.37 Characterization and Optimizat
Page 95 and 96: of this result, ethyl acetate extra
Page 97 and 98: 3.45 Chemical Composition of The Ex
Page 99 and 100: leaves, the contents of phenolics a
Page 101 and 102: Key words: Antifungal, Armoracia ru
Page 103 and 104: Abstract: A light
Page 107 and 108: 4.5 Formation of Hawthorn, Crataegu
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Page 111 and 112: 4.13 Organic Cultivation and Use of
Page 113: 4.18 Effect of Some Chemical and Bi
Page 117 and 118: 4.25 Antioxidants In Vitro Androgen
Page 119 and 120: mortality increased from 39.66 % at
Page 121 and 122: 5.7 The use of seaweed extract of P
Page 123 and 124: 5.11 Antioxidant and Antifeedant Ac
Page 125 and 126: carveol and δ- selinene, but inhib
Page 127 and 128: inhibition 95%. In the during of bi
Page 129 and 130: (Sulzer). All experiments were cond
Page 133 and 134: capturing genetic diversity before
Page 137 and 138: 7.5 Heavy Metal Content in Hypericu
Page 139 and 140: 8.3 Development of Medicinal Plants
Page 141 and 142: 8.7 Industrial Products from Indige
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Page 145 and 146: products. The applied mulberry frui
Page 147 and 148: 9.10 The Importance of Gentiana lut
Page 151 and 152: 10.4 Comparative efficacy of variou
Page 153 and 154: 10.8 Effect of Rhus coriaria L. in
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Page 157 and 158: 10.15 Comparative Supplemented Vita
Page 159 and 160: A. DJEBARA (66) A. John De Britto (
Page 161 and 162: Eftimova Jarmila (58) EL Tahir A (1
Page 163 and 164: Mushtaq Ahmad (143) N. Gourine (35)
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Zinchenko, Iryna (52) ZRIRA Saadia
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