Abstract Book - 3rd International Symposium on Medicinal Plants ...

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educing power and DPPH radical scavenging of methanolic extracts of the three plant parts (roots, leaves and flowers). It shows that the roots are richer than the other two parts with total phenolic 214.91 mg/100g DW, tracking flowers and leaves. Flavonoids and anthocyanins in flowers are dominant, while condensed tannins are a majority in the roots. The test of reducing power indicates greater activity of the extracts of leaves, knowing that they are quantitatively less rich in phenolic compounds. Similarly to the scavenger effect against DPPH radical with IC50 of 9.98, 10.08 and 19.32 mg/100 ml for the leaves, flowers and roots, respectively. On the other hand, acute toxicity tests classify infusions of the three parts of the plant substances in the categories of "practically nontoxic" or "relatively safe" with LD 50> 10 g / Kg. The results of subacute toxicity at 1 g / Kg showed elevated levels of transaminase "AST" in the three groups that received infusions of Thapsia garganica and elevated blood urea in the lots receiving preparations from roots and leaves. It also follows that these infusions exert a pro-inflammatory and analgesic effect Key words: Thapsia garganica methanolic extract, phenolic. 1.17 Evaluation of Antioxidant Activity and Stability of Morinda citrifollia Fruit Extract Borisut P 1 ., Tansirikongkol A 1 ., Sucontphunt A 2 ., Chansriniyom C 2 . and Nimmannit Ubonthip 2 1 Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmaceutical Sciences, Chulalongkorn University. 2 National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Klongluang, Pathumthani 12120, Thailand. <strong>Abstract</strong>: The present investigation focused on the method of extraction to get the highest antioxidant activity of Morinda citrifollia (noni) fruit extract. The chemical stability and safety of noni fruit extract were also studied. The noni fruit is a multiple fruit with pungent odor when ripening. Noni fruit has been used as medicinal plant from ancient time. Researches on the phytochemical constituents and biological activity revealed that compounds in noni are beneficial for skin health. The fruit juice was freeze-dried. The residue was further extracted by ethanol, acetone and ethyl acetate. All of the extracts were determined their activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging. The extract which showed highest activity was further partition by hexane and ethyl acetate. The IC 50 was determined using DPPH assay. The safety evaluation was determined by MTT assay using keratinocyte (HaCaT). The chemical stability was determined by HPLC. Extracts were incubated at ambient, 4°C and 40°C for 2, 4 and 6 months and protected from light. The active constituents in noni fruit extract which used as a marker was scopoletin. The ethanol extraction showed the highest antioxidant activity of 0.24 mg/ml at the first step of extraction then it was chosen for further partition by hexane and ethyl acetate. The IC 50 value for DPPH assay of ethyl acetate extraction was 0.014 mg/ml. The extract concentration of 1.2 mg/ml was reported as same as 100% control cell viability value. For chemical stability determination, the scopoletin was detected at 12.9 min using HPLC. The concentration of scopoletin in the ethyl acetate extraction showed no significant reduction after incubation at ambient, 4°C and 40°C for 2, 4 and 6 months. Ethanol was used for extraction of the residue from noni fruit, then hexane and ethyl acetate was used for further extraction. The antioxidant activity of the ethyl acetate extract was 0.014 mg/ml. The safety concentration of extract was 1.2 mg/ml. It was stable at ambient temperature, 4°C and 40°C protected from light for 6 months. Key words: Antioxidant activity, fruit extract, Morinda citrifollia, stability. 9
1.18 Preparation and Characterization of Some Chlorinated 3, 5-Diaryl-2- Pyrazolines Analogue of Natural Product as Antimicrobial Agents Boufas Sihem 1 and Benmekhbi Lotfi 2 1 Université 20 Août 1955, Skikda. 2 Université de Msila. Algeria. <strong>Abstract</strong>:Variously substitued pyrazolines and their derivatives analogue of natural product isolated from various medicinal plants are important biological agents and a significant amount of research activity has been directed towards this class; their prominent effects are antimicrobial 1 antifungical antiviral, antiparasitic, anti-tubercular 2 and insecticidal agents. A series of chlorinated 3, 5-diaryl-2-pyrazolines has been synthesized by the reaction of appropriately substituted chlorochalcones (analogue of flavonoids) and phenyl hydrazine in hot acetic acid solution. The structures of all compounds have been elucidated by microanalysis and H- and C- NMR spectroscopic measurements. The antibacterial activites of the synthesized compounds against staphylococcus aures ATCC 6538, Escherichia coli ATCC 8739, klebsiella pneumoniae ATCC 4352, pseudomonas aeruginosa ATCC 1539, proteus mirablis, were tested using disk diffusion method and the minimum inhibitory concentration (MIC) Method. All the déterminations tests were performed in triplicate and the results were taken as a mean of at least three determinations. The tested compounds exhibited different degrees of antibacterial activities or inhibition actions. Key words: Antimicrobial agents, characterization, chlorinated 3,5-diaryl-2-pyrazolines analogue, natural Product 1.19 Total Polyphenolic Contents and Antioxidant Activity of Santolina chamaecyparissus Bouriche Hamama, Benbrinis Soumia and Senator Abderrahmane Department of biochemistry, Faculty of Natural Sciences and Life, University Ferhat Abbas, Setif. <strong>Abstract</strong>: Currently, efforts have been put to identify natural compounds that can act as suitable antioxidants to replace synthetic ones. These naturally occurring antioxidants can be formulated as functional foods and nutraceuticals that can help to prevent oxidative damage occurring in the body. Herbal products in experimental screening methods are important to establish the active component and appropriate plant extracts. In this study, the total polyphenolic and flavonoid compounds were determined in the aqueous and methanolic extracts of Santolina chamaecyparissus by using the Folin-Ciocalteau and colorimetric aluminum chloride methods, respectively. Moreover, the antioxidant potential of these extracts was evaluated. The total polyphenolic compounds in the aqueous and methanolic extracts were 73.84 µg gallic acid equivalents/mg and 105.88 µg gallic acid equivalents/mg, respectively. While, the total flavonoids were 12.81 µg quercetin equivalent/mg and 20.99 µg quercetin equivalent/mg, respectively. At 50 µg/ml, aqueous and methanolic extracts inhibited linoleic acid peroxidation by 64% and 61%, respectively. These values are very close to that obtained with the standard antioxidant, BHT. On the other hand, the two extracts exhibited good but lower reducing power. Based on the results obtained, the extracts from Santolina chamaecyparissus may be valuable natural antioxidant sources and are potentially applicable in both medicine and healthy food industry. Key words: Antioxidant, extract, flavonoids, nutraceuticals. 10
Page 1 and 2: The 3 rd International</str
Page 3 and 4: المملكت األردنيت ا
Page 5 and 6: Welcome Dear Participants, It is a
Page 7 and 8: SCIENTIFIC COMMITTEE - Mohammad San
Page 9 and 10: INVITED SPEAKERS Prof. Dr. Alain Me
Page 11 and 12: 1.3 Antioxidant Activity of Thapsia
Page 13 and 14: 1.7 High Inhibition Effect on Lipid
Page 15 and 16: products are being used as a source
Page 17: Key words: Anti-microbia, antioxida
Page 21 and 22: 1.22 Eucalyptus globulus: Polypheno
Page 23 and 24: 1.26 Antioxidant and Antiulcerogeni
Page 25 and 26: hydroxyl radical scavenging and red
Page 27 and 28: 1.34 Anti-Inflammatory Effects of H
Page 29 and 30: 1.38 Evaluation of the Antidiabetic
Page 31 and 32: general pain. It can be used as bev
Page 33 and 34: activity, respectively. Two yeasts,
Page 35 and 36: concentrate; n = 111 versus 10 %; n
Page 37 and 38: highest TPC was observed in HZ ecot
Page 39 and 40: 1.56 Acute and Chronic Toxicity of
Page 41 and 42: Ergosterol Found in Mushrooms and Y
Page 43 and 44: potential antimicrobial activity ag
Page 45 and 46: (11.3%). The antioxidant activity o
Page 47 and 48: pulicaria incisa (L) DC has antibac
Page 49 and 50: 1.75 The Effect of Aloe Vera Leaf G
Page 51 and 52: 1.79 Antioxidant Activity of the Aq
Page 53 and 54: 4 minutes in room temperature. Thir
Page 55 and 56: 1.86 Hepatoprotective and Hypolipid
Page 57 and 58: ingredient were developed. Nano-nio
Page 59 and 60: 167,3 ± 3,72 min), comparable to G
Page 61 and 62: disease induced by AlCl3 on the pyr
Page 63 and 64: The 3 rd International</str
Page 65 and 66: sclerosis (limb), sore throat, toot
Page 67 and 68: 2.9 Traditional Pharmacy - Attar's
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2.12 Traditional Uses and Scientifi
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2.16 Survey of Medicinal Plants Fro
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2.20 Ethnobotanical Study of Medici
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The 3 rd International</str
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3.5 Comparison between Polyphenol C
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according to ISO 3632.The knowledge
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as 89,007±1,914 % and 59,639±3,82
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egard to isoflavone concentration a
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evaluation were included 4 species
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non-serpentine, ехеpt of content
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1 Sciences Chimiques de Rennes, Cat
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guidelines came into operation in t
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3.37 Characterization and Optimizat
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of this result, ethyl acetate extra
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3.45 Chemical Composition of The Ex
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leaves, the contents of phenolics a
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Key words: Antifungal, Armoracia ru
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Abstract: A light
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4.5 Formation of Hawthorn, Crataegu
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silydanin ,) while MS media supplem
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4.13 Organic Cultivation and Use of
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4.18 Effect of Some Chemical and Bi
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cultured in vitro. The WHITE and DC
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4.25 Antioxidants In Vitro Androgen
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mortality increased from 39.66 % at
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5.7 The use of seaweed extract of P
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5.11 Antioxidant and Antifeedant Ac
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carveol and δ- selinene, but inhib
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inhibition 95%. In the during of bi
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(Sulzer). All experiments were cond
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capturing genetic diversity before
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7.5 Heavy Metal Content in Hypericu
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8.3 Development of Medicinal Plants
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8.7 Industrial Products from Indige
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evaluation improvement (P
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products. The applied mulberry frui
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9.10 The Importance of Gentiana lut
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10.4 Comparative efficacy of variou
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10.8 Effect of Rhus coriaria L. in
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perforatum L.), sage (Salvia offici
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10.15 Comparative Supplemented Vita
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A. DJEBARA (66) A. John De Britto (
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Eftimova Jarmila (58) EL Tahir A (1
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Mushtaq Ahmad (143) N. Gourine (35)
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Zinchenko, Iryna (52) ZRIRA Saadia
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