Abstract Book - 3rd International Symposium on Medicinal Plants ...

The 3 rd <strong>International</strong> <strong>Symposium</strong> on Medicinal Plants, their Cultivation and Aspects of Uses
Topic (10): Medicinal Plants in Ethno-Veterinary Practice and Animal Nutrition
10.1 The Effect of Supplementing Different Levels of Fenugreek Seeds
(Trigonella foenum– graecum) on Physiological Performance of Japans
Quail (Cutornix-cutornix Japonica) Exposed to Oxidative Stress
Induced By Hydrogen Peroxide
Ahmed T.Taha and Ahmed A. AL Douri
Animal resources dep./ College of Agriculture/ University of Tikrit/Iraq.
<strong>Abstract</strong>: The objectives of this study were to investigate the effect of oxidative stress induced
by H2O2 (0.5%) supplemented with drinking water on the physiological performance of(cutornixcutornixja
ponica) birds In addition of study the effect of Fenugreek seeds to reduced this type of
stress . Oxidative stress were determined by measuring the level of glutathione (GSH) and
malondialdehyde (MDA) in the liver tissues, and by measuring GOT and GPT enzymes activity , in
addition in the some physiological and hematological parameters. Sixty (Cutornix-cutornix
Japonica) birds were randomly assigned in to six treatments groups as follows: (control group T1)
The birds were given standard diet and normal water, Treatment (2) The birds were given the
same ration as treatment (1) and H2O2 (0.5%) in drinking water. Treatment (3) The birds were
given a standard ration and 5 gm grounded fenugreek /Kg feed + H2O2 (0.5%) in drinking water.
Treatment (4) The birds were given a standard ration +10gm grounded fenugreek /Kg diet + H2O2
(0.5%) in drinking water. Treatment (5) The birds were given a standard ratio + 5gm grounded
fenugreek /Kg diet + normal water. Treatment (6) The birds were given a standard ratio + 10gm
grounded fenugreek /Kg diet + normal water. The result of study showed that H2O2
supplementation caused deterioration in the antioxidant status as represented by an increase in
MDA and a decrease in GSH levels of the liver tissue . Significant increases (P≤0,05) in WBC,
glucose ,cholesterol and GOT, GPT enzymes activities blood serum .An improvement of the
antioxidant status of liver tissue were evident in birds receiving Fenugreek seeds as represented
through an increase of GSH and decrease in MDA levels , and significant increases (P≤0,05) in
RBC , Hb, total protein concentration. It was concluded from the present study that supplementing
(H2O2) resulted in an increase in the oxidative stress of (Cutornix-cutornix Japonica) birds; the
fenugreek seeds have ability to reduce this type of oxidative stress.
Key words: Fenugreek, japans quail, hydrogen peroxide, oxidative stress.
10.2 Influence of Drinking Water Supplementation with Licorice Extract on
Certain Blood Traits of Broilers during Heat Stress
Al-Daraji, Hazim J.
Department of Animal Resource, College of Agriculture, University of Baghdad, Baghdad, Iraq
<strong>Abstract</strong>: This study was conducted to determine the physiological mechanisms expected to be
involved in alleviating the detrimental effects of heat stress on broiler chickens by supplementing
drinking water with licorice extract (LE). A total of 600 broiler chicks, one day old were used. Birds
have been assigned to 4 treatment groups with 3 replicates of 50 chicks each (150 chicks per
treatment). Birds in the first treatment (T1) were provided with drinking water alone and considered
as a control group, whereas birds in treatments 2 (T2), 3 (T3) and 4 (T4) were provided with
drinking water supplemented with 150, 300 or 450 mg LE / liter, respectively. At the third week of
age, birds in all treatments were exposed to heat stress (38 – 43 º C) for 6 hours daily (1200 h –
1800 h).Hematological traits included in this study were: Erythrocyte counts (RBC), hemoglobin
concentration (Hb), thrombocyte counts (Thr), leucocyte counts (WBC), heterophil / lymphocyte
ratio (H / L ratio), hematocrit (pcv) and plasma uric acid (Uri), glucose (Glu), cholesterol (Cho),
Protein (Pro), aspartate aminotransaminase activity (AST), alkaline phosphatase activity (ALP),
calcium (Cal) and phosphorus (Pho). Results revealed that inclusion of the LE in the drinking water
of broiler chickens (T2, T3, and T4) exposed to heat stress resulted in significant (p < 0.05)
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