Abstract Book - 3rd International Symposium on Medicinal Plants ...

shows the dominance of the phenylpropanoids derivatives. (E)-anethol (59.4%) was the main
constituent identified in the essential oil, followed by estragol (21.2%) and dodecanal (4.4%). The
antibacterial activities of the Essential oils were assayed by using the agar diffusion method on
Echerichia coli, Salmonella enteritidis and Staphylococcus aureus.
Key words: Antibacterial, Apiaceae, Daucu reboudii coss., essential oil, GC/MS, (E)-anethol.
1.92 Antifungal Activity of Extract of Pistacia atlantica
Taxanna, A., F. Thameur., R. mohsen Maha, and Larous.L.
Faculty of natural science and life, university of Farhat abasse, Algeria.
<strong>Abstract</strong>: Antifungal activity of essentials oils and polyphenols of Pistacia atlantica leaves
collected in locality from Djelfa(algeria) was tested in vitro on six isolates of fungi species
(Aspergillus flavus, Aspergillus niger, Apergillus parasiticus, Alternaria alternata, Fusarium
moniliforme, Mauginiella scaettae). Diffusion discs method was used for antifungal activity of
essentials oils and polyphenols. The essential oils showed antifungal activity against Fusarium
moniliforme and Mauginiella scaettae at the concentration of 10-20ul/ml. In another hand ,the
isolate of Aspergillus flavus showed resistance to essentials oils tested. The polyphenols showed
strong activity against Mauginiella scaettae and Fusarium moniliforme at concentration of 10-
20ul/ml in the presence of polyphenols results showed that an Antifungal activity against Alternaria
alternata with MIC lower than 12ug/ml and MFC 12ug/ml. and Mauginiella scaettae,Fusarium
moniliforme with a MIC lower than 50ug/ml and MFC more than 50ug/ml . Aspergillus parasiticus
showed resistance to polyphenols.
Key words: Antifungal activity, essentials oils, leaves, Pistacia atlantica , polyphenols.
1.93 Antioxidant, Anti-lipid Peroxidation and Anti-Hemolytic Effect of
Centaurea calcitrapa L. Extracts
TRABSA H., BAGHIANI A., BOUSSOUALIM N., KRACHE I., BOUMERFEG S.
CHAREF N., and ARRAR L.
Laboratory of Applied Biochemistry; Department of biochemistry Faculty of natural and life
sciences, University Ferhat Abbas of Setif.
<strong>Abstract</strong>: In the present study, aerial part of Centaurea calcitrapa L. were extracted with solvent
of varying polarity allowed their separation into three main subfractions, the analysis of methanol
crud (CrE), chloroform (ChE) and ethyl acetate (EaE) extracts, showed that the EaE contains the
highest amount of flavonoids (50,71 ± 0,65 mg Eq Rutin / g dry extract and 31,96 ± 0,39 mg Eq
Quercetin / g dry extract), followed by ChE (30,96 ± 0,55 mg ER / g dry extract and 19,38± 0,33
mg EQ / g dry extract), and CrE with 27,29 ± 0,18 mg ER / g dry extract and 17,16 ± 0,11 mg EQ /
g dry extract. The β-carotene / linoleic acid bleaching assay revealed that the extracts have a very
important antioxidant activity. The results showed that CrE has the highest antioxidant activity,
followed by EaE and ChE with 95.00 ± 3,48 %, 86,43 ± 2,48 % and 80,44 ± 0,19 %, respectively.
Using DPPH assay, the highest activity was observed with EaE (IC50 = 0,037 ± 0,0006 mg / ml),
followed by CrE and ChE with IC50 of 0,109 ± 0,0009 and 0,290 ± 0,0053 mg / ml, respectively.
The antioxidant activities of the CrE is confirmed by an in vivo assay in mice, using two doses
orally administrated: Cr dose 1 (CrD1: 50 mg/kg/day), Cr dose 2 (CrD2: 100 mg/kg/day) during 21
days. Total antioxidant capacity of plasma and red blood cells was measured by using DPPH
radical and from the kinetics of hemolysis, respectively. All treated groups compared with native
control (GCtl) and the treated with vitamin C (GVit C) groups, CrD2 group showed a strong
scavenging activity using DPPH assay (51,64 ± 7,82 %), higher than that of GVit C (47,27 ± 6,78
%) and CrD1 group (45,95 ± 6,26 %). The half-life (HT50), which corresponds to 50% of cell lysis
was calculated from the kinetics of hemolysis obtained, the results showed that both groups
treated with plant extract had a protective effect against erythrocytes hemolysis (CrD2: HT50=
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