Abstract Book - 3rd International Symposium on Medicinal Plants ...

highest TPC was observed in HZ ecotype with 323 mg/100g (mg equivalent of gallic acid
(GAE)/100g) while BG showed the lowest TPC (198mg GAE/100g). The antioxidant activity of
pearl millet flour significantly varied among ecotypes and ranged from 10.7% to 25.5%. Pearl millet
ecotype (HZ) showed the highest antioxidant activity while (BG) ecotype exhibited the lowest.
Therefore, pearl millet holds a good potential as a source of bioactive components. Research on
endogenous bioactive components such as phenolic compounds will contribute to unleash the
capacity of pearl millet to be the cornerstone of food security in Tunisia as well as in many
developing countries.
Key words: Antioxidant, cholesterol, Pearl millet, polyphenol.
1.53 Study of Extracts Plants on Crystallisation Calcium Phosphate Stones
Urinary In Vitro
M. Beghalia 1,2 , S. Ghalem1, H. Allali 1 , A. Belouatek 2 , H.Allali 1
1 LASNABIO. Department of Chemistry, Faculty of Sciences, University Aboubekr Belkaïd BP 119,
Tlemcen, Algeria. 2 University Centre of Relizane, Algeria.
<strong>Abstract</strong>: In this work, we performed an in vitro crystallization study enabling the specification of
kinetic and thermodynamic conditions of formation and growth of crystalline calcic phosphates
species by . We used inhibitors, which are medicinal plants which prevent, slow down or reduce
crystallization phases. We chose the classical model for the study of phosphate crystallization
without inhibitor and with it, in order to assess the inhibiting capacity of any chemical species used.
The precipitation of the solid phase of phosphates from artificial urine. The crystal size
development was monitored by polarized microscopy at different time intervals. After crystallization
time, the mixture was filtered, the recovered dried precipitates were analysed by FTIR
spectroscopy and X-rays diffraction technique and chemical analysis. In the absence of inhibitor,
the crystallization of phosphates led to the formation of struvite and amorphous carbonated
calcium phosphates (ACCP), after 6 hours. In presence of inhibitor, at lower concentrations of
plant inhibition was partial. The addition of 1 ml of extract plant to the mixture decreases the size
of crystal, after 4 hours the size of crystals stabilized at 20.67 μm. The complete disappearance of
struvite crystals was obtained after addition of 10mL of inhibitor plant, only Pentahydrated
octocalcic phosphates (POP) and ACCP were formed. In the presence of other inhibitor extract
plant, the inhibition of struvite growth and aggregation increased. The addition of up to a volume of
20 mL of the second inhibitor resulted in total inhibition and crystalline transformation of the ACCP
into carbapatite (CA). Phosphate compounds encountered in urine can be dangerous and the use
of inhibitors to prevent, slow down or reduce crystallization phases might be very helpful. In this
investigation, extracts plants proved to be good inhibitors. Their effect increases with solution pH
but they are more efficient in less acidic or neutral urine than in alkaline one.
Key words: Crystallization, inhibition; IR, medicinal plants, struvite.
1.54 The Anti-Psoriatic Effects of Hypericum triquetrifolium and Peganum
harmale -Derived Factors are Mediated by Down Regulation of Proinflammatory
Cytokines and up Regulation of Apoptosis
Mahajna Shihnaz 1 , Hadieh Bahaa 1 , Zaid Hilal 1 , Abo Farich Basheer 1 , Soroka
Yoram 3 , Said Omar 2 and Saad Bashar 1,4
1 Al-Qasemi Research Center- Al-Qasemi Academic College. 2 Antaki Center for Herbal Medicine
Ltd, Kufur Kanna. 3 The Dead Sea Laboratory for Skin Biochemistry and Biotechnology in Ein-Gedi.
4 Faculty of Art and Sciences, Arab American University Jenin, Palestine.
<strong>Abstract</strong>: Psoriasis is a common cutaneous disorder characterized by inflammation and
abnormal epidermal proliferation. Several observations indicate that cytokines produced within the
skin are of major importance in the pathogenesis of psoriasis. Overexpression of proinflammatory,
type 1 cytokines has been demonstrated in psoriasis and is believed to be of pathophysiological
28