Abstract Book - 3rd International Symposium on Medicinal Plants ...

HMQC, HMBC, DEPT and MS studies. The compound has never been reported previously from
this plant.
Key words: Flavone glucoside, rutaceae, Salvadora persica, spectroscopic data.
3.23 Chemical Constituents of Cachrys libanotis L.
Kamel Medjroubi 1 , Nabila Bouderdra 1 , Abdelhakim Elomri 2 , Philippe Vérité 3 ,
and Elisabeth Seguin 2
1 Laboratoire de Phytochimie et Analyses Physico-chimiques et Biologique, Département de
Chimie, Faculté des Sciences Exactes, Université Mentouri de Constantine, Route d’Aïn El Bey
25000 Constantine Algérie. 2 Université de Rouen, CNRS UMR 6014,C.O.B.R.A. - I.R.C.O.F., UFR
Médecine-Pharmacie, 22 Boulevard Gambetta, 76183 Rouen cedex 1, France. 3 Université de
Rouen, ADEN EA 4311, UFR Médicine-Pharmacie, Laboratoire de Chimie Analytique, 22
Boulevard Gambetta, 76183 Rouen cedex 1, France.
<strong>Abstract</strong>: Seven known coumarins and flavonoid glycoside, osthole, Meranzin hydrate, 8-
methoxy marmesin, 3‟-methoxy dihydroseseletin, nodakenin, 8-hydroxy nodakenin, Meranzin
hydrate- 3‟-O-ı-glucopyranoside, hesperidin, were isolated from aerial parts of Cachrys libanotis.
Their structures were determined by 1 and 2-D NMR techniques.
Key words: Apiaceae, Cachrys libanotis, coumarins, flavonoid glycoside.
3.24 Determination of Flavonoids and Tannins in some Serpentine
Hypericum Populations fom Bulgaria
Krasteva Ilina 1 , Pavlova Dolja 2 , Pencheva Ivanka 3 , Nedelcheva Anely 2 ,
Zdraveva Petranka 1 , and Nikolov Stefan 1
1 Department of Pharmacognosy, Faculty of Pharmacy, Medical University, 2 Department of Botany,
Faculty of Biology, University of Sofia,. 3 Department of Pharmaceutical Chemistry, Faculty of
Pharmacy, Medical University, Sofia, Bulgaria.
<strong>Abstract</strong>: Serpentine habitats are naturally rich in potentially cytotoxic elements like Ni, Fe, Mg,
Co which exert strong selective pressures on the native vegetation. The most species growing on
serpentine are adapted to these special conditions in different ways. In this study we investigate 7
populations of three Hypericum species (H. cerastoides, H. aucheri and H. montbretii) growing on
serpentines in Bulgaria to evaluate: 1) tannins and flavonoids content in the aboveground plant
parts; 2) differences between species and their populations growing on and off serpentine. Aerial
parts of Hypericum species were collected during the flowering season in June 2010 from
serpentine, distributed in Rhodope Mts. The total quantity of the tannins and flavonoids are
determined by spectroscopic method (European Pharmacopoeia). The presence of rutin in the
samples was established by HPLC analysis. The results of the quantitative analysis of flavonoids
and tannins in serpentinite populations were compared with literature data for non-serpentinite
populations. Total amount of flavonoids in the serpentinite samples varies from 0.82% to 1.54%
and in non-serpentinite – 0.74-1.22%. The results show that the content of flavonoids in
serpentinite populations was higher than non-serpentine. In the most of the studied serpentinite
samples were found higher levels of tannins than non-serpentinite. Only in H. cerastoides the
content of tannins is lower than non-serpentinite. HPLC analysis detected the presence of rutin in
the serpentinite populations from H. montbrıtii and H. cerastoides. In populations of H. aucheri the
content of rutin is in trace. The high amount of rutin was found in populations of H. cerastoides. In
populations of H. montbretii the content of rutin is lower than in H. cerastoides. The study is still in
progress. Comparisons between rutin content in serpentine and non-serpentine populations are
impending. The serpentine populations show constantly high levels of flavonoids and tannins than
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