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Abstract Book - 3rd International Symposium on Medicinal Plants ...

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increase in RBC, Hb, Thr, WBC, PCV, and plasma Uri, Glu, AST, ALP, Ca and Pho and significant<br />

(p < 0.05) decrease in H / L ratio, and plasma Cho and Pro compared with (T1). However, T4<br />

recorded the best results with relati<strong>on</strong> to all blood characteristics included in this experiment. It<br />

was c<strong>on</strong>cluded from this study that supplementati<strong>on</strong> of LE particularly at the level of 450 mg / liter<br />

to the drinking water of broiler chickens exposed to heat stress can depress the adverse effects of<br />

heat stress <strong>on</strong> blood characteristics of broiler chickens. Therefore, LE can be used as an effective<br />

feed additive for enhance general physiological status of birds during heat stress.<br />

Key words: Broiler, erythrocyte, heat stress, licorice extract, plasma, thrombocyte, water.<br />

10.3 Effect of Garlic Oil Supplementati<strong>on</strong> <strong>on</strong> Laying Hens Fed with T-2 Toxin<br />

C<strong>on</strong>taminated Diet<br />

Ancsin Zsolt, Erdélyi Márta, Balogh Krisztián, Bócsai Andrea and Mézes<br />

Miklós<br />

Department of Nutriti<strong>on</strong>, Faculty of Agricultural and Envir<strong>on</strong>mental Sciences, Szent István<br />

University, H-2103 Gödöllõ, Hungary.<br />

<str<strong>on</strong>g>Abstract</str<strong>on</strong>g>: The aim of present study was to investigate whether garlic essential oil, with natural<br />

organosulfur compound c<strong>on</strong>tent, that possesses free radical scavenging activity, is able to<br />

eliminate the adverse effects of T-2 mycotoxin c<strong>on</strong>taminati<strong>on</strong> in laying hens‟ diet. Hens were<br />

divided randomly into three treatment groups: C<strong>on</strong>trol (C), C<strong>on</strong>trol + T-2 toxin (T) and C<strong>on</strong>trol + T-<br />

2 toxin + Garlic essential oil (GT) (n=15 in each group). The C<strong>on</strong>trol group (C) was fed with<br />

commercial broiler diet without added antioxidants (basal diet). In the case of group T the basal<br />

diet was experimentally c<strong>on</strong>taminated with T-2 toxin with a dosage of 2.05 mg/kg feed. In group<br />

GT the feed of T group was complemented with 0.30 g/kg feed garlic essential oil. Experimental<br />

diets were fed for 21 days. During this period, the egg producti<strong>on</strong> was recorded daily, and egg<br />

samples for quality measurements were taken <strong>on</strong> weekly basis. On day 21 five randomly selected<br />

birds from each group were exterminated, and blood, liver, muscle, kidney and spleen samples<br />

were taken to determine the c<strong>on</strong>centrati<strong>on</strong> of mal<strong>on</strong>dialdehyde (MDA), as meta-stable end product<br />

of free radical initiated lipid peroxidati<strong>on</strong>, and that of the reduced glutathi<strong>on</strong>e (GSH), the most<br />

important sulfhydryl c<strong>on</strong>taining low molecular weight antioxidant, as well as the activity of<br />

glutathi<strong>on</strong>e peroxidase (GPx). There were no significant changes in egg producti<strong>on</strong> and in the<br />

biochemical parameters of blood samples. However, significant changes were found in egg quality<br />

parameters as we measured, such as significantly lower egg-length (p

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