Abstract Book - 3rd International Symposium on Medicinal Plants ...

everse MDR of cancer. This lecture will highlight our laboratory‟s recent work on the bioassayguided
isolation of antitumor natural products from Hungarian plants, and a discussion about
future perspectives of antitumor natural product research will also be included.
Key words: Asteraceae, cancer, euphorbiaceae, Lamiaceae, natural compounds, polygonaceae,
rutaceae.
1.40 Evaluation of the Ex Vivo Antimalarial Activity of Guava Leaf Extract
(Psidium guajava) on Erythrocytes Infected-Plasmodium berghei NK 65
Ishak Shafariatul Akmar and Ariffin Noraniza
Program Of Biomedical Science, Academic Centre Of Applied Science And Diagnostic Science,
Faculty Of Health Sciences , National University Of Malaysia , Kuala Lumpur
<strong>Abstract</strong>: Malaria is the most destructive and dangerous parasitic disease. The burden of this
disease is getting worse, mainly due to the resistance of Plasmodium falciparum against most of
the current antimalarial drugs. Therefore, the search for new antimalarial drug is urgently needed.
Thus, in this studies extract of Psidium guava leaves seems to be promising for treatment of
malaria infections. Crude extracts of guava leaves were prepared according to method known as
cold extraction using different solvent polarity which are hexane, etil asetat, aseton and methanol.
These extracts were evaluated against infected erythrocytes with Plasmodium berghei NK65. The
Plasmodium Lactate Dehydrogenase (pLDH) assay was employed for determination of the
antimalarial activity (IC 50 ) after 24 hours treatment at concentration range of 10-0.0000001 mg/ml.
Plasmodium berghei NK65 was also cultured in synchronization process to produce a specific
stage of morphology of trophozoite and schizont. Initially the study was done for screening most
effective solvent at parasitemia of 10%, the result showed that methanol extract was the most
effective which IC 50 is 4.270 mg/ml approximately to IC 50 values of the gold standard chloroquine
drug which is 3.080 mg/ml. To prove it, one way ANOVA statistical test are used and results
showed no significant differences between them as such F (1, 18) = 1091, p> 0.05 (0.310). The
methanol extract of guava leaves also were treated on erythrocytes infected Plasmodium berghei
NK65 at different level of parasitemia which are 5%, 10% and 30% . The degrees of parasitemia
represents the severity parasite infection inside the body of an individual as well. The study found
that malaria inhibitory activities effectively in parasitemia of 10% and it is not significantly different
to the chloroquine F (1, 18) = 0797, p> 0.05 (0.384). Finally, the synchronization process was
carried out by using the sorbitol lysis method where it lysed most of the malarial stage except the
ring form (early trofozoit) and then the ring form were cultured in complete media for 24 hours in
order to obtained the trofozoites and schizont form . Results demonstarated that in the ring form
(early trofozoit), IC 50 is 0.0001 mg/ml whereas late trofozoit and skizon showed similar IC 50 value
which is 4.276 mg/ml 4.295 mg/ml. In conclusion, guava leaf extract methanol showed the
potential of anti-malarial agent.
Key words: Malaria, Plasmodium berghei, Psidium guajava, synchronization.
1.41 Antioxidant Activity of Aqueous Tea Extracts
KAABOUR Faiza, BELAMBRI Sahra Amel, BOURICHE Hamama, and
SENATOR Abderrahmene.
Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Nature and Life
Sciences, Ferhat Abbas University, Setif, 19000, Algeria.
<strong>Abstract</strong>: Overproduction of reactive oxygen species (ROS) during oxidative stress is involved
in development and progression of many diseases such as inflammation, cardiovascular diseases
and aging. The most widely used synthetic antioxidants in food industry such as BHT (butylated
hydroxytoluene), BHA (butylated hydroxyanisole) and PG (propyl galate) have been suspected to
cause or promote negative health effects. So, there is a growing interest to study natural products,
since they contain a wide variety of compounds which provide protection against harmful free
radicals. Tea derived from leaves of Camellia sinensis is the most popular drink over the world.
Traditional medicine has recommended this plant for headaches, body aches, digestion, and
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