Abstract Book - 3rd International Symposium on Medicinal Plants ...

concentrate; n = 111 versus 10 %; n = 104). The study duration was 8-10 days, with the primary
parameter (pain on active motion) tested on day 4-5. The application of the higher concentration
resulted in a significantly better and clinically highly important reduction of pain, as compared with
the reference preparation.The origin of pain was more closely inspected in a subgroup analysis,
differentiating pain caused by muscle overload (n = 135; of which n = 56 using verum) from muscle
pain caused by blunt macrotraumas (n = 75; of which n = 47 using verum). Five patients with pain
of other aetiology were not included. As in the original study, the primary statistical parameter was
the assessment of pain on active motion on study day 4-5, measured through a visual analogue
scale. A statistically highly significant advantage for verum over reference was found for both types
of pain on study day 4-5 (t-Test, p ≤ 2 10 -5 ). Pain on motion was reduced for verum versus
reference by 62, 5 % versus 19,9 % in cases of muscle overload, and by 61,8 % versus 27,7 % in
cases of blunt macrotraumas. At the same time a significantly faster onset of pain relief was
registered with verum (75.0 % versus 40.5 % quick and very quick onset in cases of muscle
overload, and 76.6 % versus 42.8 % in cases of blunt macrotraumas; Mann-Whitney-Test, p ≤ 4
10 -3 ). Topical Symphytum herb concentrate is efficacious against muscle pain originating from
muscle overload as well as from blunt macrotraumas, with a quick onset of pain relief. The effect
size must be considered clinically highly important.
Key words: Concentrate cream, muscle pain, symphytum herb, treatment.
1.49 Antioxidant Activity of Extracts of Fraxinus angustifolia
Laib Yasmina, Sebaihi S., Remila S., M., Chaher N., Debache N., Ayouni K.,
Berboucha M., Boudaoud, H., Atmani, D. and Atmani, D.
Laboratory of Applied Biochemistry; University of Bejaia, Algeria.
<strong>Abstract</strong>: In the present work, we studied the anti-radical effects of extracts of Fraxinus
angustifolia, a plant used locally to treat numerous inflammatory-related pathologies. Results
concerning phenol analysis showed different rates, the richest in total phenols and Flavonoids with
respective values of 249.01 mg CatEq/g of extract and 169.53 mg RtEq /g of extract is the ethyl
acetate extract of leaves and the richest in tannins is the ethanol extract of bark (990.4 mg TAEq
/g of extract). At a concentration of 100 µg/ml, the ethanolic leaf extract exhibited the best activity
against OH (65.68%, IC50 = 79.76µg/ml), higher than that of the standards of which the best
(catechin) showed a rate of 37.85%. Evaluation of anti-DPPH activity at a concentration of
100µg/ml proved that bark extracts showed very important activity which is higher than 94%.
Results of anti-H 2 O 2 indicate that all extract showed a high activity around 70% at 100 µg/ml; the
ethyl acetate extract of leaves showed the most important scavenging effect against this radical,
with an IC50 of 51.79µg/ml. Analysis using column chromatography revealed two active fractions:
polar F2 eluted with a mixture of ethyl acetate/methanol and F1 less polar eluted with chloroform.
Analysis of these fractions using thin layer chromatography showed that the anti-radical effect
could be probably due to the presence of rutin and tannic acid derivatives in the case of F2 of leaf
extracts and to rutin and quercetin for F2 and F1 of bark extracts. Bark F1 revealed the presence
of complex molecules with high molecular weight having an important anti-radical effect which
remains to be identified and exploited in therapy.
Key words: Antioxidant, anti-radical, chromatography, Fraxinus angustifolia.
1.50 Chemical and Biological Study of Essential Oils of Two Populations of
Algerian Daucus setifolius
Laouer Hocine 1 , Bouheda Amina 1 , Bouchekrit Moufida 1 , Boulachaab
Nacira, Sahli Farida and Akkal S 2 .
1 Laboratory of natural resources valorization, University of Setif, Algeria. 2 Department of
chemistry, University of Mentouri, Constantine, Algeria.
<strong>Abstract</strong>: The composition of the essential oils of the aerial parts of Daucus setifolius
(Apiaceae), obtained by hydrodistillation, collected from two site in Algeria (Bejaia and Skikda),
was analyzed by GC and GC/MS. The yields of essential oils were varying from 0.3 % (Skikda) to
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